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The examination of mucin inducing COX-2 as a pathological molecule of rheumatoid arthritis.

Research Project

Project/Area Number 16590992
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 膠原病・アレルギー・感染症内科学
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

KAWAHITO Yutaka  Kyoto Prefectural University of Medicine, Graduate School of Medicine, Associate professor, 医学研究科, 講師 (50336731)

Co-Investigator(Kenkyū-buntansha) YOSHIKAWA Toshikazu  Kyoto Prefectural University of Medicine, Graduate School of Medicine, Professor, 医学研究科, 教授 (90158410)
TOKUNAGA Daisaku  Kyoto Prefectural University of Medicine, School of Medicine, Associate professor, 医学部, 准教授 (90343409)
NAKADA Hiroshi  Kyoto Sangyo University, Department of Biotechnology, Faculty of Engineering, Professor, 工学部, 教授 (90113141)
SANO Hajime  Hyogo college of medicine, School of Medicine, Professor, 医学部, 教授 (00196304)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2006: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2005: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2004: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsmucin inducing COX-2 / rheumatoid arthritis / abnormality of carbohydrate chain / COX-2 / ムチン
Research Abstract

Purpose
Abnormality of carbohydrate chain of mucin, which is enriched in synovial fluid (SF), has a possibility to relate with pathological conditions of rheumatoid arthritis (RA) such as the production of IL-1, TNF-a and PGE 2 in joints. In this study, we examine to detect a new mucin as a therapeutic target of RA and analyze its biological activity and structure.
Results
1) Immunohistochemical analysis showed that superficial synovial cells, and infiltrating inflammatory mononuclear cells in RA synovial tissues express MUC-1, Tn and sialyl Tn which are typical malignancy-associated mucins.
2) Western blot analysis revealed that SF faractions purified by gel filtration and further purified preparation by desalination and density-gradient centrifugation include MUC-1, Tn and sialyl Tn.
3) The newly discovered mucin from SF in patients with RA can stimulate the production of IL-1, TNF-a and PGE2 in human peripheral blood monocytes (PBMC).
Perspective
We revealed that MUC-1, Tn and sialyl Tn expressed in synovial tissues. We also found a new mucin purified from SF in patients with RA, which can produce cytokines and PGE2 on PBMC. This mucin may be mainly MUC-1. We are going to analyze its structure as a therapeutic target of RA and also examine the pathological mechanism including the proliferation of synovial cells and the induction of osteoclast.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (9 results)

All 2007 2006 2005

All Journal Article (9 results)

  • [Journal Article] A critical role for allograft inflammatory factor-1 in the pathogenesis of rheumatoid arthritis.2007

    • Author(s)
      Kimura M, Kawahito Y, et al.
    • Journal Title

      J Immunol. 178(5)

      Pages: 3316-3322

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A critical role for allograft inflammatory factor-1 in the pathogenesis of rheumatoid arthritis2007

    • Author(s)
      Kimura M, Kawahito Y, et al.
    • Journal Title

      J Immunol. 178(5)

      Pages: 3316-22

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Sphingosine 1-phosphate/sphingosine 1-phosphate receptor 1 signaling in rheumatoid synovium : regulation of synovial proliferation and inflammatory gene expression.2006

    • Author(s)
      Kitano M, Hla T, Sekiguchi M, Kawahito Y, et al.
    • Journal Title

      Arthritis Rheum. 54(3)

      Pages: 742-753

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Expression of peroxisome proliferator-activated receptor-gamma in renal ischemia-reperfusion injury.2005

    • Author(s)
      Matsuyama M, Kawahito Y, et al.
    • Journal Title

      Transplant Proc. 37(4)

      Pages: 1684-1685

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Study of cyclooxygenase-2 in renal ischemia-reperfusion injury.2005

    • Author(s)
      Matsuyama M, Kawahito Y, et al.
    • Journal Title

      Transplant Proc. 37(1)

      Pages: 370-372

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Copper chelation with tetrathiomolybdate suppresses adjuvant-induced arthritis and inflammation-associated cachexia in rats.2005

    • Author(s)
      Omoto A, Kawahito Y, et al.
    • Journal Title

      Arthritis Research & Therapy 7(6)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Study ofcydooxygena-2in renal ischemia-reperfusion injury.2005

    • Author(s)
      Matsuyama M, Kawahito Y, et al.
    • Journal Title

      Transplant Proc. 37(1)

      Pages: 370-372

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Expression of pcroxisomc proliferator-activated receptor-gamma in renal ischemia-reperfusion injury.2005

    • Author(s)
      Matsuyama M, Kawahito Y, et al.
    • Journal Title

      Transplant Proc. 37(4)

      Pages: 1684-1685

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Copper chelation with tetrathiomolybdate suppresses adjuvant-induced arthritis and inflammation-associated cachexia in rats.2005

    • Author(s)
      Omoto A, Kawahito Y, et al.
    • Journal Title

      Arthritis Research & Therapy 7(6)

    • Related Report
      2005 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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