Research for the medical treatment of organic fibrosis by controlling the protein interacting with connective tissue growth factor
| Project/Area Number |
16591000
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
膠原病・アレルギー・感染症内科学
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| Research Institution | Kinki University |
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Principal Investigator |
YOSHIDA Koji Kinki University, medicine, lecturer, 医学部, 講師 (60230736)
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| Co-Investigator(Kenkyū-buntansha) |
SAITO Akio Kinki University, medicine, lecturer, 医学部, 講師 (40153788)
HAYAKAWA Sumio Kinki University, medicine, assistant, 医学部, 助手 (00368292)
NAKATANI Tatsuya Kinki University, medicine, lecturer, 医学部, 講師 (40319661)
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2006: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | CTGF / fibronectin / fibrin / two-hybrid / beta2-glycoprotein I / transferrin |
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| Research Abstract |
Connective tissue growth factor (CTGF) is a member of the CCN family of the cysteine-rich proteins and involved in wound healing and fibrosis. We have previously shown a biochemical interaction between the CTGF and fibronectin (FN) using the yeast two-hybrid system. In this study, we confirmed the interaction between the CTGF and FN using the surface plasmon resonance (SPR) and solid-phase binding analysis. Our results show that the regions containing the FN type I repeat modules (the N-terminal fibrin, the gelatin-collagen and the C-terminal fibrin binding domains) of FN and the C-terminal domain of CTGF are required for the interaction. We also demonstrated that CTGF enhances the affinity of FN to fibrin. It appears that CTGF contributes to the extracellular matrix accumulation in wound healing and tissue fibrosis by enhancing the affinity of FN to fibrin. Because CTGF is up-regulated during the tissue repair and in coagulation cascade-associated fibrotic disorders, the new function of CTGF found in this study is consistent with its physiological role."
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Report
(4 results)
Research Products
(6 results)
