| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Research Abstract |
(1) About 40-50% adult CD8+ T cell were CD244 positive and CD244 positive fraction increased with aging whereas almost of newborn infant CD8+ T cell was CD 244 negative. CD28/CD62L positive, or negative two subgroup were present in CD244 positive cells when they analyzed three-color flow cytometry, whereas all CD244 negative CD8+ T cells were CD28 positive CD62L positive. Increase of CD244+ cells have been found in patients with infectious mononucleosis (IM) and measles., compared it with a normal subject, and in particular, and increase of CD28,CD62L positive CD244 positive CD8+ T cell were prominent.
(2) We analyzed the difference of expression of surface antigen and intracellular cytotoxic granular proteins in CD244-positive, and CD244-negative CD8+ T cell subgroup. CD244-negative cells showed surface phenotype almost same as naive cell from all surface molecule expression and did not show perform, granzyme B, TIA-1 expression either. On the other hand, CD244-positive cells showed me
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mory cell surface phenotype.
(3) Naive T cell have TCR repertoire with full of diversity, but T cell repertoire diversity was expected to be limited in memory-T cells, and TCR CDR spectrum were analyzed in each CD244-positive, CD244-negative cell subgroup. CD244 negative cells presented Gaussian distribution in all TCRVβ, but CD244-positive demarcation showed a skewed pattern in many TCRVβ. In addition, complexity index of CDR3 spectra in CD28-negative cell or CD62L negative cell decreased more than that in CD28 positive cells, or CD62L positive cell.
(4) CD244-negative CD8+ T cell separated from normal adult CD8+T cells were stimulated and incubated, in vitro. We confirmed conversion from CD244-negative to CD244-positive phenotypte in CD8+ T cells. CD244 expression were increased with CD3 stimulation at early stage of culture. On the other hand, switch to CD28,CD62L- phenotype took two or three weeks and progressed in slowly.
(5) We measured TREC content in each cell fractionation to quantify degree of mitosis in functional differentiation phase.
CD 244 negative cells showed abundant TREC content, and the TREC content decreased in order of CD28CD62L positive CD244-positive cells, CD28/CD62L negative CD244-positive cell populations. Less
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