| Project/Area Number |
16591022
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Nagoya University |
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Principal Investigator |
KIMURA Hiroshi Nagoya University, Graduate School of Medicine, Associate, 大学院・医学系研究科, 助教授 (30303621)
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| Co-Investigator(Kenkyū-buntansha) |
HAYAKAWA Masahiro Nagoya University, Hospital, Assistant Professor, 医学部附属病院, 講師 (40343206)
MORISHIMA Tsuneo Okayama University, Graduate School of Medicine, Professor, 大学院・医歯薬学総合研究科, 教授 (90157892)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2005: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2004: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | Cytomegalovirus / preterm infant / breast milk / real-time PCR assay |
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| Research Abstract |
Objective : Recently, it is reported that transmission of CMV via breast milk frequently occurred in preterminfants. However, our previous study indicated that the transmission rate was only 10% and none had symptomatic infection. The cause of contradictable result might be that our study population was relatively high gestational age. Therefore, we evaluated the rate of postnatal transmission via breast milk from mothers to extremely preterm infants.
Method : This study population comprised breast-fed preterm infants with birth weight of less than 1000g and/or gestational age of less than 28 weeks. Breast milk, donor milk, serum, and urine samples were collected every 2 weeks until discharge, and screened for CMV infection using real-time PCR assay. Most of breast milk had been preserved at -20 degrees centigrade before feeding to the infants.
Result : Twenty-five mothers and 27 infants enrolled. CMV IgG was positive in 21 mothers. All of the 21 seropositive mothers had detectable CMV-DNA in their breast milk during the study period. Of the 27 infants who were fed CMV-DNA positive breast milk, CMV infection was confirmed in only one infant. The infant had no clinical symptom except for mild elevation of transaminase.
Conclusion : Despite the high rate of CMV-DNA in breast milk, infection rate in the extremely preterm was very low. The finding of this study might be associated with the method of breast milk preservation. CMV infections transmitted via breast milk did not much impact on extremely preterm infants in our institute.
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