| Project/Area Number |
16591081
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Embryonic/Neonatal medicine
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
IMAMURA Shin-ichiro Tokyo Women's Medical University, School of Medicine, Assistant, 医学部, 助手 (00176497)
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| Co-Investigator(Kenkyū-buntansha) |
HAYAMA Emiko Tokyo Women's Medical University, School of Medicine, Assistant, 医学部, 助手 (00349698)
MATSUOKA Rumiko Tokyo Women's Medical University, School of Medicine, Assistant Professor, 医学部, 講師 (50120051)
NAKANISHI Toshio Tokyo Women's Medical University, School of Medicine, Associate Professor, 医学部, 助教授 (90120013)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2004: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | Ductus Arteriosus / Heart / DSCAM / K Channel / PCR / Porcine / Rat / Rabbit |
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| Research Abstract |
DSCAM : We tried to obtain the DSCAM (Down Syndrome Cell Adhesion Molecule) gene from the cDNA library that originated from newborn porcine ductus arteriosus. In addition, using newborn and fetal rats and rabbits, we tried to obtain the DSCAM gene not only from the ductus arteriosus but also from the aorta, pulmonary artery and heart muscle. The results showed that there might be a difference in expression time (developmental stage) and species specificity of the DSCAM gene. Furthermore, the possibility that there is a different protein structure in the DSCAM in each tissue was suggested. It is important to examine in detail how this gene is related to the development of the cardiovascular system, since it is a gene that undergoes various alternative splicings in different situations.
Kv channel : Kv1.5 expression was high in the main pulmonary artery (PA) and third and fourth branches of the PA (b-PA) but low in the ductus arteriosus (DA). Kv9.3 expression was also high in the b-PA but low in the DA. Kvβ1.2 expression was high in the DA but low in the main PA and b-PA. Expression levels of Kv1.2, Kv2.1, Kvβ1.3, and Kvβ1.4 were low in these blood vessels. Kvβ1.2 strongly inactivated the Kv1.5 currents expressed in Xenopus oocytes. These findings indicate that Kvβ1.2 in the DA might inactivate the Kv1.5 current effectively because of the higher expression of Kvβ1.2. This inactivation might lead to smooth muscle cell depolarization, opening of calcium channels, an increase in intracellular calcium, and vasoconstriction. The DA is known to constrict with an increase in oxygen at birth but the PA does not. This opposite response to oxygen might result from the difference in the relative expression levels of the Kv1.5 and Kvβ1.2.
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