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Analysis of candidate genes of atopic dermatitis based on microarray analysis of atopic skin lesions : polymorphism of Serpin peptidase inhibitor, clade B, member 8 in Japanese atopic dermatitis patients.

Research Project

Project/Area Number 16591096
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionShiga University of Medical Science

Principal Investigator

SUGIURA Hisashi  Shiga University of Medical Science, Department of dermatology, assistant professor in part time job, 医学部, 非常勤講師 (00162868)

Co-Investigator(Kenkyū-buntansha) SATOU Hiroshi  Shiga University of Medical Science, Department of Biology, professor, 医学部, 教授 (90090430)
YAMAMOTO Kazuo  Shiga University of Medical Science, Department of Internal Medicine, assistant professor in part time job, 医学部, 非常勤講師 (10314163)
UENISHI Toshiaki  Shiga University of Medical Science, Department of Dermatology, assistant professor, 医学部, 講師 (80242981)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2005: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2004: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsatopic dermatitis / gene analysis / microarray / keratinocyres / SNPs / SERPINB8
Research Abstract

we examined polymorphism of some genes associates with keratinocytes protein expression in patients with atopic dermatitis. (Methods) One hundreds and twenty eight patients with atopic dermatitis and 86 healthy controls were included. We extract DNA from peripheral blood lymphocytes from these individuals. According to the data base, some SNPs in the Serpin peptidase inhibitor, clade B, member 8 (SERPINB8), CP-2,NDST-1,Gli-1,TRK-A, MGLL, MCM2 were examined by direct sequence methods. After then, we also perform RFLP methods in SERPINB8. (Results) No significant difference was found in SNPs of CP-2,NDST-1,Gli-1,TRK-A, MGLL, MCM2 genes by direct sequence screening methods between 20 cases of atopic patients and 20 control subjects. In SERPINB8, we found significant difference at G203A and R68Q. The Allele frequency for SERPINB8 G203A variants SERPINB8 was 0.177 in control population (n=86) and 0.285 in atopic dermatitis patients (n=128). Homozygotes for SERPINB8 G203A G/G were observed in 0.053% of control population and 0.093% of atopic dermatitis patients. (Discussion) SERPINB8 locates in 18q21 which is one of the candidate loci for atopic dermatitis. SERPINB8 is one of potent serine proteinase inhibitor and strongly expressed in differentiated keratinocytes. Polymorphism of SERPINB8 G2O3A effect amino acid translation and might be concern with terminal differentiation of keratinocytes in patients with atopic dermatitis.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (13 results)

All 2006 2005 2004

All Journal Article (13 results)

  • [Journal Article] Combined effect 25-hydroxycholesterol and IL-1 β on IL-18 production in human colon carcinoma cell line (Caco-2)2006

    • Author(s)
      Bingxue Bai
    • Journal Title

      Inflammation (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Combined effect 25-hydroxycholesterol and IL- 1 □ on IL-18 production in human colon carcinoma cell line (Caco-2)2006

    • Author(s)
      Bingxue Bai
    • Journal Title

      Inflammation (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Combined effect 25-hydroxycholesterol and IL-1β on IL-18 production in human colon carcinoma cell line (Caco-2)2006

    • Author(s)
      Bingxue Bai, Kazuo Yamamoto, Hiroshi Sato, Hisashi Sugiura, Toshihiro Tanaka
    • Journal Title

      Inflammation (In press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Large-scale DNA microarray analysis of atopic skin lesions shows overexpression of an epidermal differentiation gene cluster in the alternative pathway and lack of protective gene expression in the cornified envelope.2005

    • Author(s)
      Sugiura Hisashi
    • Journal Title

      Br J Dermatol. 152-1

      Pages: 146-9

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Association between RANTES promoter polymorphism -401A and enhanced RANTES production in atopic dermatitis patients.2005

    • Author(s)
      Bingxue Bai
    • Journal Title

      J Dermatol Sci 39

      Pages: 189-91

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] アトピー性皮膚炎の治療マーカー : 皮膚乾燥とフィラグリン2005

    • Author(s)
      杉浦久嗣
    • Journal Title

      皮膚の科学 4巻増刊5号

      Pages: 12-16

    • NAID

      130004934424

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Large-scale DNA microarray analysis of atopic skin lesions shows overexpression of an epidermal differentiation gene cluster in the alternative pathway and lack of protective gene expression in the cornified envelope.2005

    • Author(s)
      Sugiura Hisashi
    • Journal Title

      Br J Dermatol Jan ; 152(1)

      Pages: 146-149

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Association between RANTES promoter polymorphism -401A and enhanced RANTES production in atopic dermatitis patients.2005

    • Author(s)
      Bingxue Bai
    • Journal Title

      J Dermatol Sci. Sep Sep ; 39(3)

      Pages: 189-191

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Association between RANTES promoter polymorphism -401A and enhanced RANTES production in atopic dermatitis patients.2005

    • Author(s)
      Bingxue Bai, Keiko Tanaka, Takahiro Tazawa, Naoko Yamamoto, Hisashi Sugiura
    • Journal Title

      J Dermatol Sci 39

      Pages: 189-191

    • NAID

      10018031953

    • Related Report
      2005 Annual Research Report
  • [Journal Article] アトピー性皮膚炎の治療マーカー:皮膚乾燥とフィラグリン2005

    • Author(s)
      杉浦久嗣, 中野雅子
    • Journal Title

      皮膚の科学 4巻増刊5号

      Pages: 12-16

    • NAID

      130004934424

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Large-scale DNA microarray analysis of atopic skin lesions shows over-expression of an epidermal differentiation gene cluster in the alternative pathway and lack of protective gene expression in the cornified envelope2005

    • Author(s)
      H Sugiura, H Ebise*, T Tazawa, K Tanaka, Y Sugiura§, M Uehara, K Kikuchi*, T Kimura*
    • Journal Title

      British Journal of Dermatology 152

      Pages: 146-149

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Relative importance of IL-4 and IL-13 in lesional skin of atopic dermatitis2004

    • Author(s)
      Tazawa Takahiro
    • Journal Title

      Arch Dermatol Res 295

      Pages: 459-64

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Relative importance of IL-4 and IL-13 in lesional skin of atopic dermatitis.2004

    • Author(s)
      Tazawa Takahiro
    • Journal Title

      Arch Dermatol Res. Apr ; 295(11)

      Pages: 459-464

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary

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Published: 2004-04-01   Modified: 2016-04-21  

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