| Project/Area Number |
16591096
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
SUGIURA Hisashi Shiga University of Medical Science, Department of dermatology, assistant professor in part time job, 医学部, 非常勤講師 (00162868)
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| Co-Investigator(Kenkyū-buntansha) |
SATOU Hiroshi Shiga University of Medical Science, Department of Biology, professor, 医学部, 教授 (90090430)
YAMAMOTO Kazuo Shiga University of Medical Science, Department of Internal Medicine, assistant professor in part time job, 医学部, 非常勤講師 (10314163)
UENISHI Toshiaki Shiga University of Medical Science, Department of Dermatology, assistant professor, 医学部, 講師 (80242981)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2005: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2004: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | atopic dermatitis / gene analysis / microarray / keratinocyres / SNPs / SERPINB8 |
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| Research Abstract |
we examined polymorphism of some genes associates with keratinocytes protein expression in patients with atopic dermatitis. (Methods) One hundreds and twenty eight patients with atopic dermatitis and 86 healthy controls were included. We extract DNA from peripheral blood lymphocytes from these individuals. According to the data base, some SNPs in the Serpin peptidase inhibitor, clade B, member 8 (SERPINB8), CP-2,NDST-1,Gli-1,TRK-A, MGLL, MCM2 were examined by direct sequence methods. After then, we also perform RFLP methods in SERPINB8. (Results) No significant difference was found in SNPs of CP-2,NDST-1,Gli-1,TRK-A, MGLL, MCM2 genes by direct sequence screening methods between 20 cases of atopic patients and 20 control subjects. In SERPINB8, we found significant difference at G203A and R68Q. The Allele frequency for SERPINB8 G203A variants SERPINB8 was 0.177 in control population (n=86) and 0.285 in atopic dermatitis patients (n=128). Homozygotes for SERPINB8 G203A G/G were observed in 0.053% of control population and 0.093% of atopic dermatitis patients. (Discussion) SERPINB8 locates in 18q21 which is one of the candidate loci for atopic dermatitis. SERPINB8 is one of potent serine proteinase inhibitor and strongly expressed in differentiated keratinocytes. Polymorphism of SERPINB8 G2O3A effect amino acid translation and might be concern with terminal differentiation of keratinocytes in patients with atopic dermatitis.
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