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Analysis of the mechanisms of cell proliferation by T-caclherin in skin prolit~rative diseases

Research Project

Project/Area Number 16591097
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionKyoto University

Principal Investigator

MATSUYISHI Norihisa  Kyoto University, Graduate School of Medicine, Lecturer, 医学研究科, 講師 (10263071)

Co-Investigator(Kenkyū-buntansha) TODA Ken-ichi  Kitano Hospital, Department of Dermatology, Director, 北野病院・皮膚科, 部長 (80159045)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥2,600,000 (Direct Cost: ¥2,600,000)
KeywordsT-cadherin / inhibition of cell proliferation / suqamous cell carcinoma / transfection / RNA interference / cell cycle / 増殖抑制 / 扁平上皮癌細胞株
Research Abstract

T-cadherin is a unique member of cadherin superfamily that lacks transmembrane daomain and cytoplasmic domain. And instead of those domains, it linked to the plasma membrane via a glycosyl-phoshatidylinositol anchor. Cytoplasmic domain of classical cadherin is crucial for its cell-cell adhesion, and the lack in T-cadherin means the existence of another function except for adhesion. The fact that T-cadherin expression is downregulated in cell proliferative disorders including squamous carcinoma or psoriasis vulgaris suggests that T-cadherin is associated with cell proliferation. In this study, we established two kinds of cell lines. One is T-cadherin overexpressed human squamous carcinoma cell line (HSC-1) by transfection method and another is T-cadherin knock down HSC-1 by RNAi method. We anlysed the effect of T-cadherin on proliferation using previous cell lines and intact HSC-1 cells, and found that T-cadherin was negative regulator of cell proliferation. We also confirmed the result using tetracycline inducible trasfectant. This function was cased by the dely of cell proliferation cycle at G2/M phase. These results suggets that T-cadherin is a key molecule of cell proliferation mechanisms in human squamous carcinoma cells.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (4 results)

All 2005

All Journal Article (4 results)

  • [Journal Article] T-cadherin negatively regulates the proliferation of cutaneous squamous carcinoma cells.2005

    • Author(s)
      Yohei Mukoyama
    • Journal Title

      The Journal of Investigative Dermatology 124

      Pages: 833-838

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] T-cadherin cegatively regulates the proliferation of cutaneous squamous caricinoma cells2005

    • Author(s)
      Yohei Mukoyama
    • Journal Title

      The Journal of Investigative Dermatology Vol 124

      Pages: 833-838

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] T-Cadherin Negatively Regulates the Proliferation of Cutaneous Squamous Carcinoma Cells2005

    • Author(s)
      Yohei Mukoyama
    • Journal Title

      The Journal of Investigative Dermatology 124

      Pages: 833-838

    • Related Report
      2005 Annual Research Report
  • [Journal Article] T-cadherin negatively regulates the proliferation of cutaneous squamous carcinoma cells2005

    • Author(s)
      Mukoyama Y, Zhou S, Miyachi Y, Matsuyoshi N
    • Journal Title

      The Journal of Investigative Dermatology 124・4

      Pages: 833-838

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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