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Analysis of Immunoregulation mechanism in recalcitrant allergic skin lesions and the development of new immunothrapy

Research Project

Project/Area Number 16591108
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionYokohama City University

Principal Investigator

IKEAZAWA Zenro  Yokohama City University, Graduate School of Medicine, Professor, 大学院・医学研究科, 教授 (90046128)

Co-Investigator(Kenkyū-buntansha) TAKESHITA Yoshihiro  Yokohama City University, School of Medicine, Assisiant, 医学部, 助手 (80381507)
NAKAZAWA Masatoshi  Yokohama City University, School of Medicine, Assceiale_Professor, 医学部, 准教授 (20217699)
蒲原 毅  横浜市立大学, 医学部, 助手 (70347293)
猪又 直子  横浜市立大学, 医学部, 助手 (20347313)
相原 道子  横浜市立大学, 医学部, 助教授 (90231753)
高橋 一夫  横浜市立大学, 医学部, 講師 (40264618)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2005: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2004: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsAtopic dermatitis(AD) / Model mouse of AD / Endocrine disruptor, Tributyltin / Zinc deficiency food / Nerve growth factor / Semaphorin / Regulatory T cells / Supplement therapy / アトピー性皮膚炎の動物モデル / Cyclophosphamide / 環境ホルモントリブチルスズ / 免疫療法 / アトピー性皮膚炎 / ADの自然発症モデルマウスNC / Nga / ADの自然発症モデルマウスDs-Nh / CpGモチーフ / Cyclophosphamide(Cy) / IFN-γ / IL-13 / CD25+CD4+調節T細胞
Research Abstract

1) Nerve growth factor (NGF) in the horny layer proteins taken fron the skin surface by stripping with sticky tape, correlated with skin symptoms of atopic dermatitis (AD), number of peripheral blood eosinophils and grade of itching, and also estimated as indicator of the efficacy of antiallergic drugs against itching of AD.
2) Several biomarkers reflecting symptoms of AD were detected by analysis of horny layer proteins, and the basis of development of new test system, which can detect rapidly and easily the skin function of AD, was established.
3) The effect of semaphorin, which is supposed to suppress the extension of peripheral nerve was examined in the experimental AD-like dermatitis.
4) The induction of oral tolerance were linked with the increase of FoxP3+CD25+T cells and its breakdown linked with the decrease of CD25+CD4+ regulatory T cells. Then, their clinical application has been studied.
5) The zinc deficiency induced and enhanced AD-like dermatitis in the experimental animal model mouse of AD. Then, the supplement effect of zinc on development of AD-like dermatitis has been studied.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (34 results)

All 2007 2006 2005

All Journal Article (34 results)

  • [Journal Article] アトピー性皮膚炎の経過における角層内神経成長因子の定量的検討と治療の影響2007

    • Author(s)
      山口絢子, 相原道子, 小林雄輔, 池澤善郎
    • Journal Title

      The Proceeding of 32^<nd> JSID (投稿中)

      Pages: 141-141

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 亜鉛欠乏食におけるアトピー自熱発症モデルマウスDS-Nhの皮疹悪化の免疫学的解析2007

    • Author(s)
      高橋日奈子, 中澤正年, 高橋一夫, 相原道子, 平沢勉, 南睦彦, 池澤善郎
    • Journal Title

      The Proceeding of 32^<nd> JSID (投稿中)

      Pages: 142-142

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of tributyltin on the emotional behavior of C57BL/6 mice and the development of atopic dermatitis-like lesions in DS-Nh mice2007

    • Author(s)
      Aihara M, Ohtaki K, Takahashi H, Fujita H, Takahashi K, Funabashi T, Hirasawa T, Ikezawa Z
    • Journal Title

      Journal of Dermatological Science Vol.46(3)(In press)

    • NAID

      10024116982

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] トリブチルスズ経口摂取による腸管における経口免疫寛容への影響2007

    • Author(s)
      内田敬久, 中澤正年, 藤巻春香, ほか
    • Journal Title

      Journal of Environmental Dermatology and Cutaneous Allergology(JEDCA) Vol.1(2)(In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 2,4-dinitrofluorobenzene(DNFB)の経口投与により誘導されるハプテン特異的経口免疫寛容に対するcyelophosphamideの効果2007

    • Author(s)
      立脇聡子, 中澤正年, 相原道子, ほか
    • Journal Title

      JEDCA Vol.1(1)

      Pages: 12-21

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] ハプテンによる経鼻免疫寛容の誘導とインフルエンザウイルス感染による経鼻感作の増強2007

    • Author(s)
      中村和子, 中澤正年, 相原道子, 佐藤文孝, 高橋一夫, 南 陸彦, 池澤善郎
    • Journal Title

      JEDCA Vol. 1(1)

      Pages: 22-30

    • NAID

      10024293101

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of cyclophosphamide on 2, 4-dinitrofluorobenzene-specific oral tolerance2007

    • Author(s)
      Tatewaki S, Nakazawa M, Aihara M, Hongo N, Hotta Ch, Ikezawa Y, Takahashi K. Minami M, Ikezawa Z
    • Journal Title

      J Environ Dermatol Cutan Allergol 1(1)

      Pages: 12-21

    • NAID

      10024293081

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Intranasal tolerance was induced by nasal administration of hapten and influenza A virus infection enhanced the sensitization to nasal administration of hapten2007

    • Author(s)
      Nakamura K, Nakazawa M, Aihara M, Sato F, Takahashi K. Minami M, Ikezawa Z
    • Journal Title

      J Environ Dermatol Cutan Allergol 1(1)

      Pages: 22-30

    • NAID

      10024293101

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Ingestion of tributyltin induced atorophy of Pyer patch, decreased production of IL-10 by Pyer patch cells, and, might induce breakdown of oral tolerance2007

    • Author(s)
      Uchida T, Nakazawa M, Fujimaki H, Takahashi K. Minami Ikezawa Z
    • Journal Title

      J Environ Dermatol Cutan Allergol 1(2), (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of tributyltin on the emotional behavior of C5711/6 mice and the development of atopic dermatitis-like lesions in DS-Nh mice,2007

    • Author(s)
      Aihara M, Ohtaki K, Takahashi H, Fujita I-1, Takahashi K, Funabashi T, Hirasawa T, Ikezawa Z
    • Journal Title

      J Dermatol Sci (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] アトピー性皮膚炎の経過における角層内神経成長因子の定量的検討と治療の影響2007

    • Author(s)
      山口絢子, 相原道子, 小林雄輔, 池澤善郎
    • Journal Title

      The Proceeding of 32^nd JSID (投稿中)

      Pages: 141-141

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 亜鉛欠乏食におけるアトピー自然発症モデルマウスDS-Nhの皮疹悪化の免疫学的解析2007

    • Author(s)
      高橋日奈子, 中澤正年, 高橋一夫, 相原道子, 平沢勉, 南睦彦, 池澤善郎
    • Journal Title

      The Proceeding of 32^nd JSID (投稿中)

      Pages: 142-142

    • Related Report
      2006 Annual Research Report
  • [Journal Article] トリブチルスズ経口摂取による腸管における経口免疫寛容への影響2007

    • Author(s)
      内田敬久, 中澤正年, 藤巻春香ほか
    • Journal Title

      Journal of Environmental Dermatology and Cutaneous Allergology(JEDCA) Vol.1(2)(In press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 2,4-dinitrofluorobenzene(DNFB)の経口投与により誘導されるハプテン特異的経口免疫寛容に対するcyclophosphamideの効果2007

    • Author(s)
      立脇聡子, 中澤正年, 相原道子ほか
    • Journal Title

      JEDCA Vol.1(1)(In press)

    • NAID

      10024293081

    • Related Report
      2006 Annual Research Report
  • [Journal Article] ハプテンによる経鼻免疫寛容の誘導とインフルエンザウイルス感染による経鼻感作の増強2007

    • Author(s)
      中村和子, 中澤正年, 相原道子, 佐藤文孝, 高橋一夫, 南 睦彦, 池澤善郎
    • Journal Title

      JEDCA Vol.1(1)(In press)

    • NAID

      10024293101

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Effects of photocatalytic agent on DS-Nh mice, developing atopic dermatitis-like eruption with an increase of Staphylococcus aureus.2006

    • Author(s)
      Kambara T, Aihara M, Matsukura S, Sato I, Kubota Y, Hirasawa T, Ikezawa Z
    • Journal Title

      Int Arch Allergy Immunol 141

      Pages: 151-157

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of tributyltin on development of atopic dermatitis-like eruptions in DS-Nh mice.2006

    • Author(s)
      Nomura C, Aihara M, Nakazawa M, Takahashi K, Yoshioka N, Matstukura S, Hirasawa T, Minami M, Ikezawa Z
    • Journal Title

      Int Arch Allergy Immunol 141

      Pages: 337-345

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of bamboo leaf extract on atopic dermatitis model mice2006

    • Author(s)
      Fujita H, Takahashi H, Aihara M, Hirasawa T, Ikezawa Z
    • Journal Title

      J Environ Dermatol 13

      Pages: 87-94

    • NAID

      10018174051

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A novel method to control the balance of skin microflora. Part 1. Attack on biofilm of Staphylococcus aureus without antibiotics.2005

    • Author(s)
      Katsuyama M, Ichikawa H, Ogawa S, Ikezawa Z.
    • Journal Title

      J Dermatol Sci. 38 (3)

    • NAID

      10018031120

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Cyclophosphamide decreases the number, percentage and the function of CIO+ 04+ regulatory T cells, which suppress induction of contact hypersensitivity2005

    • Author(s)
      Ikezawa Y, Nakazawa M, Tamura C, Takahashi K, Minami M, Ikezawa Z.
    • Journal Title

      J Dermatol Sci. 39 (2)

      Pages: 105-12

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of TNCB sensitization in DS-Nh mice, serving as a model of atopic dermatitis, in comparison with NC/Nga mice, Int Arch Allergy Immunol, 136(2) : 173-180, 2005.2005

    • Author(s)
      Matsukura S, Aihara M, Hirasawa T, Ikezawa Z
    • Journal Title

      Int Arch Allergy Immunol 136(2)

      Pages: 173-180

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Hyperproduction of IFN by CpG oligodeoxynucleotide-induced exacerbation of atopic dermatitis-like skin lesion in some NC/Nga mice2005

    • Author(s)
      Takakura M.Takeshita F.Aihara M, }din Ke-Qin, Ichino M, Okuda K, Ikezawa Z
    • Journal Title

      J Invest Dermatol 125 (6)

      Pages: 1156-1162

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of TNCB sensitization in DS-Nh mice, serving as a model of atopic dermatitis, in comparison with NC/Nga2005

    • Author(s)
      Matsukura S, Aihara M, Hirasawa T, Ikezawa Z
    • Journal Title

      Int Arch Allergy Immunol 136(2)

      Pages: 173-180

    • Related Report
      2005 Annual Research Report
  • [Journal Article] A novel method to control the balance of skin microflora. Part 1. Attack on biofilm of Staphylococcus aureus without antibiotics.2005

    • Author(s)
      Katsuyama M, Ichikawa H, Ogawa S, Ikezawa Z.
    • Journal Title

      J Dermatol Science 38(3)

      Pages: 197-205

    • NAID

      10018031120

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Cyclophosphamide decreases the number, percentage and the function of CD25+ CD4+ regulatory T cells, which suppress induction of contact hypersensitivity2005

    • Author(s)
      Ikezawa Y, Nakazawa M, Tamura Ch, Takahashi K, Minami M, Ikezawa Z
    • Journal Title

      J Dermatol Science 39(2)

      Pages: 105-112

    • NAID

      10018031622

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Hyperproduction of IFN-□ by CpG oligodeoxynucleotide-induced exacerbation of atopic dermatitis-like skin lesion in some NC/Nga mice2005

    • Author(s)
      Takakura M, Takeshita F, Aihara Mi, Xin Ke-Q, Ichino M, Okuda K, Ikezawa Z
    • Journal Title

      J Invest Dermatol 125(6)

      Pages: 1156-1162

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Feeding with tributyltin diet strongly promotes the early development of atopic dermatitis (AD)- eruptions with an increase of IL-13 production by TNCB topical application in Ds-Nh, model mice of AD,2005

    • Author(s)
      Iwamura Ch, Nakazawa M, Minami M, Takahashi K, Yoshioka N, Hirasawa T, Ikezawa Z
    • Journal Title

      Int Arch Allergy Immunol (revised)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Feeding with tributyltin diet promotes Th2-polarized allergy in mouse through a decreasing effect on innate immunity and CD25^+CD4^+ regulatory T cells2005

    • Author(s)
      Iwamura Ch, Nakazawa M, Takahashi K, Ikezawa Z, Minami M
    • Journal Title

      Eur J Immunol (revised)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Feeding with tributyltin diet strongly promotes the early development of atopic dermatitis (AD)- eruptions with an increase of IL-13 production by TNCB topical application in Ds-Nh, model mice of AD2005

    • Author(s)
      Iwamura Ch, Nakazawa M, Minami M, Takahashi K, Yoshioka N, Hirasawa T, Ikezawa Z
    • Journal Title

      Int Arch Allergy Immunol (投稿予定)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] CD25+ CD4+ regulatory T cells suppress the cyclophosphamide-induced enhancement of atopic dermatitis (AD)- eruptions with an increase of IL-13 and IFNγ production in Ds-Nh, model mice of AD2005

    • Author(s)
      Ikezawa Y, Nakazawa M, Matsukura S, Yoshioka N, Hirasawa T, Minami M, Ikezawa Z
    • Journal Title

      Int Arch Allergy Immunol (投稿予定)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Hyperproduction of IFN-γ by CpG oligodeoxynucleotide-induced exacerbation of atopic dermatitis-like skin lesion in some NC/Nga mice2005

    • Author(s)
      Takakura M, Takeshita F, Aihara Mi, Xin Ke-Q, Ichino M, Okuda K, Ikezawa Z
    • Journal Title

      J Invest Dermatol (sumitted)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Feeding with tributyltin diet promotes Th2-polarized allergy in mouse through a decreasing effect on innate immunity and CD25^+ CD4^+ regulatory T cells2005

    • Author(s)
      Iwamura Ch, Nakazawa M, Takahashi K, Ikezawa Z, Minami M
    • Journal Title

      Eur J Immunol (sumitted)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Cyclophosphamide decreases the number, percentage and the function of CD25+ CD4+ regulatory T cells, which suppress induction of contact hypersensitivity2005

    • Author(s)
      Ikezawa Y, Nakazawa M, Tamura Ch, Takahashi K, Minami M, Ikezawa Z
    • Journal Title

      J Dermatol Science 37(In press)

    • NAID

      10018031622

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Effects of TNCB sensitization in DS-Nh mice, serving as a model of atopic dermatitis, in comparison with NC/Nga mice2005

    • Author(s)
      Matsukura S, Aihara M, Hirasawa T, Ikezawa Z
    • Journal Title

      Int Arch Allergy Immunol 136(2)

      Pages: 173-180

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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