| Project/Area Number |
16591244
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | TOHOKU UNIVERSITY |
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Principal Investigator |
KAWAGISHI Naoki Tohoku University, Hospital, Research Associate, 病院, 助手 (00333807)
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| Co-Investigator(Kenkyū-buntansha) |
DOI Hideyuki Tohoku University, Hospital, Associate Professor, 病院・助教授 (90188839)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2004: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | incompatible / liver transplantation / living / ELIZA / antibody titer / organ transplantation / immunosuppression / rejection / 肝臓移植 / 腎臓移植 |
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| Research Abstract |
In Japan, the shortage of grafts has encouraged us to perform living donor liver transplantation (LDLT) across the ABO blood barrier. To decide the treatment for an ABO-I recipient, the amount of anti-A/B antibody (Ab) is very important. At present, the quantity of anti-A/B Ab is represented by the agglutination titer. We developed an enzyme linked immunosorbent assay (ELISA) to measure anti-A/B Ab, and assessed its usefulness in clinical practice in this study.
In the assessment of all sera, the correlation between the O.D. and the titer of anti-A/B Ab were moderate. However, in the assessment of each recipient, the correlation between the O.D. and the titer of anti-A/B Ab was strong or moderate. Moreover, this investigation suggested that with our method it was possible to detect Ab-mediated rejection much earlier.
In conclusion, our ELISA method is useful to detect changes in individually anti-A/B Ab and may have the possibility of using ELISA in clinical cases.
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