| Project/Area Number |
16591258
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
MURAKAMI Yoshiaki Hiroshima University, Hospital, Lecturer, 病院, 講師 (10263683)
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| Co-Investigator(Kenkyū-buntansha) |
HIYAMA Eiso Hiroshima University, Natural Science Center for Basic Research and Development, Professor, 自然科学研究支援開発センター, 教授 (00218744)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2005: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2004: ¥3,300,000 (Direct Cost: ¥3,300,000)
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| Keywords | islet transplantation / hepatic regeneration / promotion of engraftment / growth factor / islet cell hypertrophy |
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| Research Abstract |
PURPOSE. With the current donor shortage, future success with islet transplantation is incumbent on the ability to use single donor islets. Manipulating the recipient graft environment may allow the use of fewer islets to achieve insulin-independence. We examined the impact of hepatic regeneration on engraftment of intraportally transplanted islet cells in the immediate post-operative period.
METHODS. Isolated pancreatic islets from a single-donor were intraportally transplanted to streptozotocin-diabetic rats immediately after 70 % partial hepatectomy (HxPIT1). Plasma glucose levels and body weight were monitored for 30 days ; intravenous glucose tolerance tests and serum insulin levels were obtained at the second and fourth weeks after transplantation ; and morphological studies were performed on insulin immunostained sections of the liver. Results from HxPIT1 rats were compared to those from rats transplanted with single-donor islet grafts (PIT1) and those from rats transplanted with double-donor islet grafts (PIT2).
RESULTS. Hyperglycemia was ameliorated in HxPIT1 and PIT2 rats after transplantation but not in PITT rats. Body weight gain of the HxPIT1 rats was more than that of the PIT1 rats. Glucose tolerance and insulin secretion in the HxPIT1 group were superior to the PIT1 group and equivalent to those in the PIT2 group. There were no significant differences comparing the glucose tolerance and insulin levels at the second week to those at the fourth week after transplantation. Morphologically, grafted islets from HxPIT1 rats were large and granulated by hypertrophic beta cells. The sizes of the grafted islets and individual insulin-stained islet cells were larger than those from PIT1 rats.
CONCLUSIONS. In streptozotocin-diabetic rats, islet transplantation performed in regenerating liver results in improved insulin secretion and serum glucose control, even in the face of a limited number of islets.
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