Establishment of an hepatocyte cell line derived from human amnio-blast cells and the evaluation of its functions when transplanted and orientation to artificial bio-liver apparatus.
| Project/Area Number |
16591274
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Jikei University School of Medicine |
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Principal Investigator |
TABEI ISAO Jikei University School of Medicine, SCHOOL of MEDICINE, CLINICAL ASSOCIATE (50266649)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIKAWA Hiroshi Nippon Dental University, School of Life Dentistry at Tokyo, Section of Developmental and Regenerative Medical Science, PROFESSOR (30089784)
HASHIMITO Hisashi THE JIKEI UNIVERSITY, SCHOOL of MEDICINE, ASSOCIATE PROFESSOR (80189498)
ISHIDA Yuichi THE JIKEI UNIVERSITY, SCHOOL of MEDICINE, ASSISTANT PROFESSOR (30260946)
大久保 辰雄 東京慈恵会医科大学, 医学部, 助手 (20277007)
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| Project Period (FY) |
2004 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,210,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2004: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | HEPATOCYTE TRANSPLANTATION / AMNION CELL / CELL CULTURE / ARTIFICIAL ORGAN / 神経原基 |
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| Research Abstract |
[Object] Although liver transplantation has become the ultimate therapy for hepatic failure patients, the lack of absolute donor organ has opened the path of development to adjuvant and/or alternative methods such as cell transplantation and artificial liver apparatus. The research to develop the material for these methods has involved fetal, embryonic stem cells and transfection, transductions of hepatocyte to become immortalized cells. But most trials are stalled due to unanswered problems involving function, tumorigensis, immunogenetic and ethical questions. The placenta is usually disposed after birth and amnion obtained from this waste expresses little MHC class I and no class II. We focused on this material that also produces other immuno-regulartory factors, hypothesizing to find immature cells that would mature into functional hepatocytes. [Methods] Human-early embryo amnion was dissected in 0.1%trypsin-0.02%EDTA/PBS (-) and cultured in growth medium (GM) containing DMEMF12+
… More
20%FBS+0.1%NEA. Small circular cells developed within the single-layered cells were cloned colonially. The cells were named HEAC cells and were used in the following experiments. HEAC cells were maintained in GM with addition of 1ng/ml of LIF. Liver failure rats were created chemically using carbon tetrachloride with the survival rate of 33%. Cells were transplanted into these rats to investigate on their protrombin time, serum albumin levels and also survival rate. Also they were cultured in collagen type 1 to characterize the differentiated cells in 3-dimension culture. [Results] Amnion cells are fairly small cells with immature cytoplasm and active differentiation potency. After several regulations the cytoplasm becomes rich with various organelle, evolving the cells to differentiate. Within the collagen sponge 3-dimention culture the cells produced Albumin, a typical characteristic of a hepatocyte. When transplanted into chemically induced liver failure rats, it improved protrombin time, serum albumin levels and also survival rate up to 90%. Less
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Report
(5 results)
Research Products
(28 results)
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[Journal Article] Treatment Of Diabetic Rats With Islet-Like Cells Derived From Early Embrionic Stem Cell And Establishing Insulin Secreating Cell Line2006
Author(s)
Isao, Tabei, Satoshi, Ohi, Yuichi Ishida, Toshiaki. Tachibana, Hisashi.Hashimoto, Kahei.Sato, Isamu.Ishiwata, Hirotaka.Kubo, Tosiaki Morikawai, Katsuhiko.Yanaga, Hiroshi. Ishikawa
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Journal Title
World Transplant Congress
Pages: 576-576
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Response to GH of Hepatocytes derived from Rat early Embrionic Stem Cell2006
Author(s)
Isao, Tabei, Satoshi, Ohi, Shigeya Kyouda,Yuichi Ishida, Toshiaki. Tachibana, Hisashi.Hashimoto, Isamu.Ishiwata, Hirotaka.Kubo, Katsuhiko.Yanaga, Hiroshi.Ishikawa
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Journal Title
Journal Of Japanese Society For Surgical Metabolism And Nutrition 40(3)
Pages: 30-30
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Characteristics of hepatocytes derived from early es cells and treatment of surgically induced liver failure rats by transplantation2005
Author(s)
I., Tabei, H., Hashimoto, I., Ishiwata, T., Tachibana, M., Akahori, S., Ohi, H., Kubo, K., Satou, Y., Yamazaki, K., Yanaga, H., Ishikawa
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Journal Title
Transplant Proceeding 37
Pages: 262-264
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Differentiation of nervous cells derived from human amniotic cells cultured with retinoic acid2005
Author(s)
I., Tabei, I., Ishiwata, K., Ninomiya, T., Hirabayashi, S. Ohi, Y., Ishida, T., Tachibana, K., Yanaga, H., Ishikawa
Organizer
23rd Annual Meeting of Japan Human Cell Society
Place of Presentation
Tokyo
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Establishment of a cell line developed from human amnion to treat hepatic failure to serve as an ideal material for artificial liver apparatus and cell transplantation2005
Author(s)
Tabei, I., Ito, R., Kyouda, S., Ohkubo, T., Ishida, Y., Tachibana, T., Hashimoto, H., Ishiwata, I., Satou, K., Kubo, H., Yamazaki, Y., Yanaga, K., Ishikawa, H
Organizer
21st Annual Meeting of Japan Human Cell Society
Place of Presentation
Tokyo
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Characteristics of hepatocytes derived from early ES cell and treatment of surgically induced liver failure rats by transplantation2004
Author(s)
I., Tabei, H., Hashimoto, I., Ishiwata, T., Tachibana, S., Ohi, H., Kubo, K., Satou, Y., Yamazaki, K., Yanaga, H., Ishikawa
Organizer
XX International Congress of The Transplantation Society
Place of Presentation
Vienna
Description
「研究成果報告書概要(欧文)」より
Related Report
