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Lymphatic Mapping and Lymphatic Endothelial Cell Isolation in Colorectal Cancer Patients

Research Project

Project/Area Number 16591298
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionSaitama Medical School

Principal Investigator

TAKEDA Akihiko  Saitama Medical University, Associate professor, 医学部, 助教授 (90312959)

Co-Investigator(Kenkyū-buntansha) OZAWA Shutaro  Saitama Medical School, Assistant Professor, 医学部, 講師 (50245221)
KOYAMA Isamu  Saitama Medical School, Professor, 医学部, 教授 (60178390)
KAWAI Yoshiko  Shinshu University, School of Medicine, Assistant Professor, 医学部, 講師 (10362112)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2004: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordssentinel lymph node / colorectal cancer / lymphatic endothelial cell / cell culture / VEGFR-3 / センチネルリンパ節生検 / 消化器癌 / センチネルリンパチャネル
Research Abstract

Background : SLN biopsy has already been established as a common procedure, and its clinical usefulness has been confirmed in patients with malignant lymphoma and breast cancer. In colorectal cancer, however, the application of the SLN theory remains uncommon and its clinical significance is also unclear. In addition, the characteristics of the lymphatic vessels that connect SLNs or the lymphatic endothelial cells have been unclear until now.
Methods : SLN biopsy by the subserous dye injection method (patent blue) was conducted in 36 patients with colorectal cancer for which curative resection was possible (stage 0 : 2 cases, stage I : 18 cases, stage II : 4 cases, stage III : 12 cases), with additional systematic lymph node dissection. Endothelial cells were isolated from lymphatic vessels identified at the time of the SLN biopsy by the collagenase II perfusion method, and we tried to transfer them into a culture system with an endothelial cell-specific medium.
Results : SLNs could be i … More dentified in 34 cases (94%). The total number of resected lymph nodes was 705, and 72 of those nodes were confirmed as SLNs (10.2%). Ten metastasis-positive nodes were found in SLNs (13.9%), and the mean number of identified SLNs per case was 2.0. The sensitivity to detect metastatic lymph nodes and the specificity of the SLN biopsy for all removed lymph nodes was 86.1% and 99.2%, respectively. No complications or toxicity associated with the dye injection were observed. In addition, cells isolated from removed lymphatic vessels formed colonies with endothelial cell-specific properties, and the expression of lymphatic endothelial cell-specific VEGFR-3 was observed.
Conclusion : The SLN biopsy by the dye method for colorectal cancer is a procedure with high sensitivity, accuracy and safety that is applicable to cases with advanced cancer. In addition, a method was established to isolate only lymphatic endothelial cells from resected lymphatic vessels and to culture them. Our results are expected to be another milestone in the determination of the rational margin of resection in colorectal surgery and the future clarification of the mechanism of lymphatic metastasis. Less

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (15 results)

All 2006 2005 2004 2003 Other

All Journal Article (15 results)

  • [Journal Article] Lymphatic Mapping and Lymphatic Endothelial Cell Isolation in Colorectal Cancer Patients2006

    • Author(s)
      Takeda A, Otani Y, Ozawa S, Ogawa N, Miyazawa M, Kawai Y, Ichioka, Shinozuka N, Koyama I
    • Journal Title

      Asian-Pacific J Clin Oncol (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] ラットリンパ内皮細胞培養法の〓取込部位別生物学的特性とリンパ管新生に関する検討2005

    • Author(s)
      河合佳子
    • Journal Title

      リンパ学 28(2)

      Pages: 74-76

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Development of rat lymphatic endothelial cell line and its biological properties2004

    • Author(s)
      Kawai Y et al.
    • Journal Title

      Microcirculation Annual 20

      Pages: 9-10

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] ZD6126 inhibits orthotopic growth and peritoneal carcinomatosis in a model of human gastric cancer2004

    • Author(s)
      MaCarty MF, Takeda A et al.
    • Journal Title

      Br J Cancer 90(3)

      Pages: 705-711

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Thrombocytosis associated with poor prognosis in patients with esophageal cancer2004

    • Author(s)
      Shimada H, Takeda A et al.
    • Journal Title

      J Am Coll Surg 198

      Pages: 737-741

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Development of rat lymphatic endothelial cell line and its biological properties.2004

    • Author(s)
      Kawai Y, Ohhashi T
    • Journal Title

      Microcirculation Annual 20

      Pages: 9-10

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] ZD6126 inhibits orthotopic growth and peritoneal carcinomatosis in a mouse model of human gastric cancer.2004

    • Author(s)
      MaCarty MF, Takeda A, Stoeltzing O, Liu W, Fan F, Reinmuth N, Akagi M, Bucana C, Mansfield, PF, Ryan A, Ellis LM
    • Journal Title

      Br J Cancer 9 ; 90 (3)

      Pages: 705-711

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Thrombocytosis associated with poor prognosis in patients with esophageal cancer.2004

    • Author(s)
      Shimada H, Oohira G, Okazumi S, Matsubara H, Nabeya Y, Hayashi H, Takeda A, Gunji Y, Ochiai T
    • Journal Title

      J Am Coll Surg 198

      Pages: 737-741

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Development of int lympbatic endothelial uo lisa and its biological poopatics2004

    • Author(s)
      Kawai. Y
    • Journal Title

      Microcianlation Ammual 20

      Pages: 9-10

    • Related Report
      2005 Annual Research Report
  • [Journal Article] ラットリンパ管内皮細胞を用いたリンパ管新生に関する検討2004

    • Author(s)
      河合佳子
    • Journal Title

      リンパ学 27(2)

      Pages: 92-96

    • Related Report
      2005 Annual Research Report
  • [Journal Article] ヒトリンパ管からのリンパ内皮細胞の直接的分離および培養法の確立2003

    • Author(s)
      河合 佳子, 竹田明彦 他
    • Journal Title

      医学のあゆみ 206.6

      Pages: 467-468

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 培養ヒトリンパ内皮細胞の増殖能2003

    • Author(s)
      河合 佳子, 竹田明彦 他
    • Journal Title

      医学のあゆみ 207.11

      Pages: 959-960

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Direct isolation and long term culture of human lymphatic endothelial cells2003

    • Author(s)
      Kawai Y, Takeda A, JE Gershenwald
    • Journal Title

      Igaku - no - Ayumi 206.6

      Pages: 467-468

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Proliferation assay for human lymphatic endothelial cells2003

    • Author(s)
      Kawai Y, Takeda A, JE Gershenwald
    • Journal Title

      Igaku - no - Ayumi 207.11

      Pages: 959-960

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Lymphatic mapping and lymphatic endothelial cell isolation in colorectal cancer patients

    • Author(s)
      Takeda A et al.
    • Journal Title

      Asia-Pacific J Clin Oncol (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary

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Published: 2004-04-01   Modified: 2016-04-21  

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