| Project/Area Number |
16591309
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
TANAKA Tatsuo Hamamatsu University Hospital, assistant professor, 医学部附属病院, 講師 (90273185)
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| Co-Investigator(Kenkyū-buntansha) |
KONNO Hiroyuki Hamamatsu Univ. Sch.of Med., professor, 医学部, 教授 (00138033)
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | Photodynamic therapy / 5-aminolevulinic acid / mono-L-aspartyl chlorine e6 (NPe6) / 光線力学療法 / 消化管癌 / 光線力学療法(PDT) / 消化器癌 / 5-アミノレブリン酸(5-ALA) / PDT / 消化器がん |
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| Research Abstract |
Photofrin is the most commonly used photosensitizer for photodynamic therapy (PDT). The major side effect of Photofrin is cutaneous photosensitivity. 5-aminolevulinic acid (5-ALA) is potentially exploitable for PDT and does not cause the side effect of prolonged normal skin photosensitization. We investigated the anti-tumor effects and safety of 5-ALA in patients with gastrointestinal cancer. 5-ALA(30 mg/kg) was administrated orally. At 4 h after administration, laser beam was irradiated to the tumor site endscopically using a directional quartz fiber (energy level : 100 J/cm^2). The antitumor effects of 5-ALA PDT according to patients were one PR and two SD. We investigated antitumor effects of 5-ALA PDT for human gastric cancer by animal model. Nude mice were implanted subcutaneously MT2 tumor (human gastric cancer line) and 5-ALA PDT was done. The rates of tumor shrinkage after PDT were 0.40 (5-ALA) and 0.16 (Photofrin). Judging from these results, anti-tumor effect of 5-ALA PDT is not satisfaction in the clinical and basic research.
Next, we investigated antitumor effects of PDT using a fractionated dosing of mono-L-aspartyl chlorine e6 (NPe6) in a murine tumor. Eight nude mice with MT2 tumor were given 10 mg/kg NPe6 intravenously. Two hours later PDT was performed using a laser diode at a light dose of 100 J/ cm^2 and wavelength of 664 nm. Histological changes in tumors were examined 3 weeks after PDT. The CR rate was 87.5 % and the overall response rate was 100 %. The anti-tumor efficacy of NPe6 for human gastric cancer is very excellent correspond to it of Photofrin.
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