| Project/Area Number |
16591315
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Kobe University |
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Principal Investigator |
FUJINO Yasuhiro Kobe University, Graduate School of Medicine, Associate Professor, 大学院医学系研究科, 助教授 (30335450)
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| Co-Investigator(Kenkyū-buntansha) |
KURODA Yoshikazu Kobe University, Graduate School of Medicine, Professor, 大学院医学系研究科, 教授 (70178143)
TANIOKA Yasuki Kobe University, Graduate School of medicine, COE Researcher, 大学院医学系研究科, COE研究員 (00372649)
SAKAI Tetsuya Kobe University, University Hospital, Assistant Professor, 医学部附属病院, 特定助手 (80372647)
鈴木 康之 神戸大学, 大学院・医学系研究科, 助教授 (40304092)
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | islet transplantation / two-layer method / I type diabetus mellitus / warm ischemia / 2層法 |
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| Research Abstract |
Exp 1 : Tew layer method (TLM) for pancreas preservation prior to islet isolation resulted in excellent islet function in addition to improved islet yield, which is almost comparable to freshly isolated islets.
Exp 2 : Tissue ATP levels after digestion were well maintained using TLM with adenosine digestion method. Greater numbers of islets could be retrieved. The new method was at least equivalent to islet function isolated by conventional method.
Exp 3 : Reduced-pressure controlled collagenase injection (RPCI) at 80 mmHg could contribute to consistently high islet yield and in vivo function in a rat model.
Exp 4 : intraperitoneal oxygenated PFC improves the outcome of intraportal islet transplantation
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