• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of hepatic reserve function after extended hepatectomy and treatment of acute liver failure induced by extended hepatectomy

Research Project

Project/Area Number 16591332
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionUniversity of Miyazaki

Principal Investigator

KAI Masahiro  University of Miyazaki, Dept. Surgery 1, Assistant Professor, 医学部, 講師 (40264379)

Co-Investigator(Kenkyū-buntansha) CHIJIIWA Kazuo  University of Miyazaki, Dept. Surgery 1, Professor, 医学部, 教授 (90179945)
KONDO Kazuhiro  University of Miyazaki, Dept. Surgery 1, Assistant Professor, 医学部, 講師 (10244196)
OHUCHIDA Jiro  University of Miyazaki, Dept. Surgery 1, Assistant Professor, 医学部, 助手 (10363590)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥2,300,000 (Direct Cost: ¥2,300,000)
Keywordsextended hepatectomy / liver failure / organic anion transporter / obstractive jaundice / bile acid
Research Abstract

Extended hepatectomy for advanced biliary cancer may result in acute liver failure even if in patients with normal liver function. Preoperative and postoperative evaluation of hepatic reserve function especially in patients with liver cirrhosis or obstructive jaundice, to prevent postoperative liver failure, is very important.
1.The changes of mRNA expression of the organic anion transporters (mrp2, mrp3, bsep, oatp and ntcp) depending on the duration of bile duct ligation have been examined in rats. Blood and liver tisues were obtained during the period from 3 to 28 days after the ligation. The excretion transporters, the mRNA levels of mrp2 and bsep decreased, while those of mrp3 increased from 3 days until 28 days after the ligation. The uptake transporters, ntcp and oatp decreased until 28 days after the ligation. After 7 days ligation of bile duct, external and internal biliary drainage were performed. The expression of mrp2 mRNA levels increased, while those of mrp3 decreased. The … More se changes of mrp2 and mrp3 mRNA expressions were significantly stronger in the internal biliary drainage than the external drainage. These suggest that the internal biliary drainage is useful for hepatic function reserve. We are now examining the expression of these protein levels.
2.We evaluated the differences in hepatic reserve function and parameters to predict the liver failure in the early stage after extended hepatectomy in rats. The survival rates of 48 hours after hepatectomy were 50% in 90% hepatectomy and 100% in 70% hepatectomy. 90% hepatectomy is a model for acute liver failure. We determined the bile flow, biliary bile acid excretion and the mRNA levels of organic anion transporters (mrp2, mrp3 and bsep) after 70% or 90% hepatectomy in rats. The bile flow and bile acid excretion both decreased significantly after the 90% hepatectomy. Biliary bile acid excretion and the expression of mrp2 decreased, while the expressions of mrp3 and bsep increased after 90% hepatectomy. In contrast, the expression of mrp2 increased, while the expressions of mrp3 and bsep decreased after 70% hepatectomy. The expressions of mrp2 and mrp3 were differently regulated after 70% and 90% hepatectomy. We suspected that expressions of mrp2 and mrp3 and biliary bile acid excretion and bile flow might be able to predict the liver failure after extended hepatectomy. Less

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (6 results)

All 2006 2005

All Journal Article (6 results)

  • [Journal Article] Biliary lipids output in the early stage of acute liver failure induced by 90% hepatectomy in the rat2006

    • Author(s)
      Tanaka, S.-I., Chijiiwa, K., Maeda, Y.
    • Journal Title

      Journal of Surgical Research (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Biliary Lipid Output in the Early Stage of Acute Liver Failure Induced by 90% Hepatectomy in the Rat2006

    • Author(s)
      Tanaka, S.-I., Chijiiwa, K, Maeda, Y.
    • Journal Title

      Journal of Surgical Research (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Risk factors for early death after liver resection in patients with solitary hepatocellular carcinoma2005

    • Author(s)
      Kondo, K., Chijiiwa, K., Makino, I., Kai, M., Maegara, N., Ohuchida, J., Naganuma,S.
    • Journal Title

      Journal of Hepatobilliary Pancreatic Surgery 12

      Pages: 399-404

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Prognostic benefit of selective portal vein Occlusion during hepatic resection for hepatocellular carcinoma.2005

    • Author(s)
      Makino, I., Chijiiwa, K., Kondo, K., Ohuchida, J., Kai,M.
    • Journal Title

      Surgery 137

      Pages: 626-631

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Risk factors for early death after liver resection in patients with solitary hepatocellular carcinoma.2005

    • Author(s)
      Kondo, K., Chijiiwa, K., Makino, I., Kai, M., Maehara, N., Ohuchida, J., Naganuma, S.
    • Journal Title

      Journal of Hepatobilliary Pancreatic Surgery 12

      Pages: 399-404

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Prognostic benefit of selective portal vein Occlusion during hepatic resection for hepatocellular carcinoma.2005

    • Author(s)
      Makino, I., Chijiiwa, K., Kondo, K., Ohuchida, J., Kai, M.
    • Journal Title

      Surgery 137

      Pages: 626-631

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary

URL: 

Published: 2004-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi