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Modification of the Hepatic Mitochondrial Proteome in Response to Ischemic Preconditioning Following Ischemia-Reperfusion Injury of the Rat Steatotic Liver.

Research Project

Project/Area Number 16591367
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionSt.Marianna University School of Medicine

Principal Investigator

NAKANO Hiroshi  St.Marianna University School of Medicine, Department of Gastroenterological Surgery, Lecturer, 医学部, 講師 (10241035)

Co-Investigator(Kenkyū-buntansha) KOBAYASHI Shinichi  St.Marianna University School of Medicine, Department of Pharmacology, Professor, 医学部, 教授 (20129836)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2004: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsSteatotic liver / Zucker rat / Ischemic preconditioning / Ischemia reperfusion / 2D-DIGE / Proteomics / Mitochoindria / 脂肪肝Zuckerラット / ミトコンドリア蛋白 / JC-1 / 70%肝切除術 / ubiquinol cytochrome C reductase core protein I / superoxide dismutase 2 / 2-cystine peroxidase
Research Abstract

Background/Aim : Ischemic preconditioning (IPC) has been shown to reduce hepatic ischemia-reperfusion (IR) injury of steatotic liver, but the effect of IPC on mitochondrial proteome has not been fully demonstrated. The present study investigated how IPC modifies the mitochondrial proteome after IR injury of the rat steatotic liver.
Methods : Zucker steatotic rats were subjected to 25-min portal triad cross-clamping (IR group). In the IPC group, 10-min temporal clamping followed by 10-min reperfusion was performed before the 25-min clamping. The other rats were sham-operated as a control group. Mitochondrial proteome was analyzed 24 hours after IR using two-dimensional differential in-gel electrophoresis and mass spectrometry.
Results : Mitochondrial inner-membrane potential and glutathione were lower and serum transaminase was higher in the IPC group than in the IR group. There was a greater degree of up-regulation of the mitochondrial precursor of aldehyde dehydrogenase 2 and alpha-methylacyl-CoA racemase in the IPC group in comparison to the IR group. In contrast, 60S acid ribosomal protein P0, carbonic anhydrase 3, arginase 1, and superoxide dismutase were significantly more down-regulated in the IPC group than in the IR group.
Conclusions : A hepato-protective effect was not shown by IPC ; however, IPC caused significant up-or down-regulation of several mitochondrial proteins 24 hours after IR of steatotic liver.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (9 results)

All 2006 2005 2004 Other

All Journal Article (9 results)

  • [Journal Article] Protein pattern difference in the colon cancer cell lines examined by two-dimensional differential in-gel electrophoresis and mass spectrometry.2006

    • Author(s)
      Katayama F, et al.
    • Journal Title

      Surg Today 36(12)

      Pages: 1085-93

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A patient undergoing pancreaticoduodenectomy in whom involved common hepatic artery anomalously arising from superior mesenteric artery was removed and reconstructed2005

    • Author(s)
      Nakano H, et al.
    • Journal Title

      Hepatogastroenterology 52

      Pages: 1883-1885

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A patient undergoing pancreatico-duodenectomy in whom involved common hepatic artery anomalously arising from superior mesenteric artery was removed and reconstructed.2005

    • Author(s)
      Nakano H, et al.
    • Journal Title

      Hepatogastroenterology 52(62)

      Pages: 1883-1885

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Endocutter no-knife and extraparenchymal control of the main hepatic veins for major hepatectomy2005

    • Author(s)
      中野 浩, 他
    • Journal Title

      Hepatogastroenterology 52(66)

      Pages: 1836-1839

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Pancreaticoduodenectomy in common hepatic artery anomalously arising from the superior mesenteric artery.2005

    • Author(s)
      中野 浩, ほか
    • Journal Title

      Hepatogastroenterology 52(66)

      Pages: 1883-1885

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Utilization of human liver microsomes to explain individual differences in naclitaxel metabolism by CYP2C8 and CYP3A4.2005

    • Author(s)
      小林 真一, 他
    • Journal Title

      J Pharmacol Sci. 97(1)

      Pages: 83-90

    • Related Report
      2005 Annual Research Report
  • [Journal Article] ミトコンドリアのプロテオミクスによる脂肪肝外科侵襲時における脆弱性についての検討2004

    • Author(s)
      中野 浩, 他
    • Journal Title

      日本外科学会雑誌 105・1

      Pages: 333-333

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Modification of the Hepatic Mitochondrial Proteome in Response to Ischemic Preconditioning Following Ischemia-Reperfusion Injury of the Rat Steatotic Liver. Eur Surg Res in press

    • Author(s)
      Oshima R, et al.
    • Journal Title

      Eur Surg Res (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Modification of the Hepatic Mitochondrial Proteome in Response to Ischemic Preconditioning Following Ischemia-Reperfusion Injury of the Rat Steatotic Liver.

    • Author(s)
      Oshima R, et al.
    • Journal Title

      Eur Surg Res (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary

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Published: 2004-04-01   Modified: 2016-04-21  

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