• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Relationship between the membrane localization of D4GDI and the cancer progression in human colon cancer cells.

Research Project

Project/Area Number 16591368
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionKanazawa Medical University

Principal Investigator

MAEDA Masayo  Kanazawa Med.Univ., School of Medicine, Assistant, 医学部, 助手 (30199632)

Co-Investigator(Kenkyū-buntansha) OTA Takahide  Kanazawa Med.Univ., Med.Res.Inst., Associate Prof., 総合医学研究所, 助教授 (10152141)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2005: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2004: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsRhoGDI / LyGDI / D4GDI / RhoGDIβ / RhoGDI2 / metastasis / colon cancer / centrosome / tight function / cell division / polarity / D4GDI / Centrosome / Tight junction / RhoGDI2 / Rho / 細胞膜 / 細胞間接着
Research Abstract

D4GDI (LyGDI/RhoGDIβ/RhoGDI2), which regulates the signaling pathways of Rho GTPases, localized at the tight junction of apical side of cell-cell interphase in a monolayer of MDCK cells and colon cancer cells which retained the epithelial morphology, but not in colon cancer cells which lost the epithelial morphology. Furthermore, D4GDI transiently localized to centrosomes in metaphase cells. These observations suggested that D4GDI played roles in the formation and/or maintenance of tight junction and in the cell division. To confirm such a possibility, we examined the phenotype of HeLa cells in which the expression of D4GDI was suppressed by siRNA more than 80%. The suppression of the expression of D4GDI increased the frequency of abnormally lobulated nuclei and the 8n and 16n cells, which were probably due to the aberrant cell division. These results confirmed that D4GDI participated in the regulation of cell division and cell polarity. D4GDI has been reported as metastasis suppressor. Our observations suggest that the decrease of the expression of D4GDI or the dysfunction of D4GDI give rise to the abnormal intercellular adhesion or the failure of cell division, thereby promote the progression of colon cancer.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (8 results)

All 2005 2004

All Journal Article (8 results)

  • [Journal Article] Overexpression of Aurora-A potentiates HRAS-mediated oncogenic transformation and is implicated in oral carcinogenesis.2005

    • Author(s)
      Tatsuka, M.
    • Journal Title

      Oncogene 24・6

      Pages: 1122-1127

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Inhibitory effect of RNAi on Japanese encephalitis virus replication in vitro and in vivo.2005

    • Author(s)
      Murakami, M.
    • Journal Title

      Microbiol. Immunol. 49・12

      Pages: 1047-1056

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Overexpression of Aurora-A potentiates HRAS-mediated oncogenic transformation and is implicated in oral carcinogenesis.2005

    • Author(s)
      Tatsuka, M., et al.
    • Journal Title

      Oncogene 24(6)

      Pages: 1122-1127

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Inhibitory effect of RNAi on Japanese encephalitis virus replication in vitro and in vivo.2005

    • Author(s)
      Murakami, M., et al.
    • Journal Title

      Microbiol.Immunol. 49(12)

      Pages: 1047-1056

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] RNAiによる日本脳炎ウイルス増殖の阻害2004

    • Author(s)
      村上 学
    • Journal Title

      金沢医科大学雑誌 29・2

      Pages: 103-108

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Nuclear translocation of cleaved LyGDI dissociated from Rho and Rac during TP53-dependent ionizing radiation-induced thymic apoptosis in vitro.2004

    • Author(s)
      Zhou, X.
    • Journal Title

      Radiation Res. 162・3

      Pages: 287-295

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Nuclear translocation of cleaved LyGDI dissociated from Rho and Rac during TP53-dependent ionizing radiation-induced thymic apoptosis in vitro.2004

    • Author(s)
      Zhou, X., et al.
    • Journal Title

      Radiation Res. 162(3)

      Pages: 287-295

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Inhibitory effect of RNAi on Japanese encephalitis virus replication.2004

    • Author(s)
      Murakami, M., et al.
    • Journal Title

      J.Kanazawa Med.Unv. 29(2)

      Pages: 103-108

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary

URL: 

Published: 2004-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi