| Project/Area Number |
16591375
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Kawasaki Medical School |
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Principal Investigator |
HIRAI Toshihiro Kawasaki Medical School, Medicine, associate professor, 医学部, 助教授 (60165149)
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| Co-Investigator(Kenkyū-buntansha) |
TSUNODA Tsukasa Kawasaki Medical School, Medicine, professor, 医学部, 教授 (00110841)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2005: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2004: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | sentinel lymph node / fluorescent beads / gastric carcinoma / 微小転移 |
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| Research Abstract |
This study examined that availability of FBM for detecting of sentinel lymph node(SN) for patients with GC. Kawasaki Medical College Institutional Review Board approved the fluorescent beads methods(FBM) for the cases of gastric carcinoma(GC) under the patient's consent.
On the day before surgery, 1ml of fluorescent beads(FB) was injected by an endoscope to four points of submucosal layer around the lesion. Ultraviolet (UV) irradiation was performed during the operation, and extracted lymph nodes were irradiated to detect SN. Light-emitting lymph nodes(LN) were assumed to be SN. At the same time, SN was detected by dye (ICG) methods injected into the subserosal layear from the serosal side surrounding a lesion at the operation time. During 15 minutes after the injection of ICG, green nodes could beb assumed to be SN. After the sampling of SN, a lymphadenectomy was done for compartments I and II. SN detection was performed for 24 patients with early GC. The SN detection rate was 75% (18/24 cases) in FBM. The average number of SN was 2.3. The SN detection rate using FB combined with ICG methods was 91.6%. The SN detection rate of FB alone was low. The FBM should be combined with ICG methods at the same time. Moreover, it was difficult to detect a fluorescent LN hidden in the adipose tissue during surgery because of autofluorescence of the adipose tissue. However, it was able to detected SN by UV irradiation to excision extraction tissue. For the purpose of application FB to clinical use in the future, a new UV irradiation instrumental should be devised to detect SN during the operation.
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