Development of small caliper artificial graft using bioreactor
| Project/Area Number |
16591414
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
MORISHIMA Katsumasa Tokyo Women's Medical University, School of Medicine, Assistant, 医学部, 助教授 (40256566)
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| Co-Investigator(Kenkyū-buntansha) |
SHIN'OKA Toshiharu Tokyo Women's Medical University, School of Medicine, Assistant Professor, 医学部, 助教授 (20192122)
NAITO Yuji Tokyo Women's Medical University, School of Medicine, Assistant, 医学部, 助手 (60328466)
KUROSAWA Hiromi Tokyo Women's Medical University, School of Medicine, Professor, 医学部, 教授 (50075511)
MATSUMURA Goki Tokyo Women's Medical University, School of Medicine, Assistant, 医学部, 助手 (20297469)
LIONG Oei Kwan Tokyo Women's Medical University, School of Medicine, Assistant, 医学部, 助手 (70372985)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | Regenerative Medicine / Tissue Engineering / Bone Marrow / Biodegradable Scaffold / Tissue-Engineered vessel / Mononuclear Bone Marrow / Bioreactor |
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| Research Abstract |
Two to 3 / kg of bone marrow cells were obtained from iliac bone of the Beagle. Then, using Ficoll technique, mononuclear bone marrow cells were collected. These cells were cultured for approximately 4 weeks in an incubator using culture flasks consist of RPMI1640, 10% FBS and antibiotics. These cells differentiated into endothelial cells, fibroblasts, and smooth muscle cells. We fabricated small caliper biodegradable scaffold using co-polymer of PLA and PCL (P(CL/LA)) to create sponge-shaped tubular graft. PGA fibers, located at inner part of the scaffold were used for reinforcement of the scaffold. Diameter of the graft was 3.5 mm, and 5 cm in length. After 4 weeks of cultivation, culture cells were trypsinized and seeded onto the scaffold following by settle up in to the bioreactor consist of pulsatile pump, tube and oxygenator. After a few weeks cultivation in the bioreactor, the cells seeded scaffold was implanted to the same dog which the bone marrow cells were obtained. The dogs were observed for 1 and 3 months after implantation. Angiograms were performed for the evaluation. One month's graft were patent ; however, they occluded with fibrotic changes within 3 months after implantation. To overcome these phenomena, we are struggling to find better material for the small caliper tissue engineering graft.
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Report
(3 results)
Research Products
(25 results)
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[Journal Article] Midterm clinical result of tissue-engineered vascular autografts seeded with autologous bone marrow cells2005
Author(s)
Shin'oka, T., Matsumura, G., Hibino, N., Naito, Y., Watanabe, M., Konuma, T., Sakamoto, T., Nagatsu, M., Kurosawa, H.
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Journal Title
Journal of Thoracic and Cardiovascular Surgery 129,6
Pages: 1330-1330
Description
「研究成果報告書概要(和文)」より
Related Report
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[Journal Article] The tissue-engineered vascular graft using bone marrow without culture2005
Author(s)
Hibino, N., Shin'oka, T., Matsumura, G., Ikada, Y., Kurosawa, H.
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Journal Title
Journal of Thoracic and Cardiovascular Surgery 129,5
Pages: 1064-1064
Description
「研究成果報告書概要(和文)」より
Related Report
-
[Journal Article] Midterm clinical result of tissue-engineered vascular autografts seeded with autologous bone marrow cells2005
Author(s)
Shin'oka, T., Matsumura, G., Hibino, N., Naito, Y., Watanabe, M., Konuma, T., Sakamoto, T., Nagatsu, M., Kurosawa, H.
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Journal Title
J Thorac Cardiovasc Surg 129,6
Pages: 1330-1330
Description
「研究成果報告書概要(欧文)」より
Related Report
-
[Journal Article] The tissue-engineered vascular graft using bone marrow without culture2005
Author(s)
Hibino, N., Shin'oka, T., Matsumura, G., Ikada, Y., Kurosawa, H.
-
Journal Title
J Thorac Cardiovasc Surg 129,5
Pages: 1064-1064
Description
「研究成果報告書概要(欧文)」より
Related Report
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