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Construction of Tissue-Engineered Vascular Autograft using Bone Marrow Derived Stem Cells and Biodegradable Scaffold and It's Clinical Application

Research Project

Project/Area Number 16591415
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Thoracic surgery
Research InstitutionTokyo Women's Medical University

Principal Investigator

MATSUMURA Goki  Tokyo Women's Medical University, School of Medicine, Assistant, 医学部, 助手 (20297469)

Co-Investigator(Kenkyū-buntansha) HIBINO Narutoshi  Tokyo Women's Medical University, School of Medicine, Assistant, 医学部, 助手 (50318094)
KUROSAWA Hiromi  Tokyo Women's Medical University, School of Medicine, Professor, 医学部, 教授 (50075511)
SHIN'OKA Toshiharu  Tokyo Women's Medical University, School of Medicine, Assistant Professor, 医学部, 助教授 (20192122)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2004: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsRegenerative Medicine / Tissue Engineering / Bone Marrow / Biodegradable Scaffold / Tissue-Engineered vessel / Mononuclear Bone Marrow / Endothelial Cells / Mechanical Properties / 生体吸収素材
Research Abstract

This study demonstrates the evaluation of the endothelial function and mechanical properties of tissue-engineered vascular autografts (TEVAs) constructed with autologous mononuclear-bone marrow cells (MN-BMCs) and a biodegradable scaffold using a canine inferior vena cava (IVC) model.
MN-BMCs were obtained from a dog and seeded onto a biodegradable tubular scaffold consisting of polyglycolide fiber and poly(1-lactide-co-ε-caprolactone) sponge. This scaffold was implanted in the IVC of the same dog on the day of surgery. TEVAs were analyzed biochemically, biomechanically and histologically after implantation. When TEVAs were explanted and stimulated with acetylcholine at 1 month, they dose dependently produced nitrates and nitrites. N^G-nitro-L-arginine methylester significantly inhibited these reactions. On the stimulation by acetylcholine, factor VIII-positive cells of TEVAs produced eNOS proteins, and the ratio of eNOS/s17 mRNA were similar among native IVC, 1-and 3-months TEVAs. TEVAs had similar biochemical properties and wall thickness as native IVC at 6 months after implantation, and tolerated venous pressure well without any problems such as calcification.
These results indicate that TEVAs are biocompatible material with functional endothelial cells and biomechanical properties without unwanted side-effects.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (25 results)

All 2005 2004

All Journal Article (22 results) Book (3 results)

  • [Journal Article] Midterm clinical result of tissue-engineered vascular autografts seeded with autologous bone marrow cells2005

    • Author(s)
      Shin'oka, T., Matsumura, G., Hibino, N., Naito, Y., Watanabe, M., Konuma, T., Sakamoto, T., Nagatsu, M., Kurosawa, H.
    • Journal Title

      Journal of Thoracic and Cardiovascular Surgery 129,6

      Pages: 1330-1330

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The tissue-engineered vascular graft using bone marrow without culture2005

    • Author(s)
      Hibino, N., Shin'oka, T., Matsumura, G., Ikada, Y., Kurosawa, H.
    • Journal Title

      Journal of Thoracic and Cardiovascular Surgery 129,5

      Pages: 1064-1064

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Midterm clinical result of tissue-engineered vascular autografts seeded with autologous bone marrow cells2005

    • Author(s)
      Shin'oka, T., Matsumura, G., Hibino, N., Naito, Y., Watanabe, M., Konuma, T., Sakamoto, T., Nagatsu, M., Kurosawa, H.
    • Journal Title

      J Thorac Cardiovasc Surg 129,6

      Pages: 1330-1330

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The tissue-engineered vascular graft using bone marrow without culture2005

    • Author(s)
      Hibino, N., Shin'oka, T., Matsumura, G., Ikada, Y., Kurosawa, H.
    • Journal Title

      J Thorac Cardiovasc Surg 129,5

      Pages: 1064-1064

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Clinical Application of Tissue-Engineered Blood Vessels2005

    • Author(s)
      Goki Matsumura, Toshiharu Shin'oka
    • Journal Title

      Cardiovascular Regeneration Therapies Using Tissue Engineering Approaches (H.Mori, H.Matsuda edition) (Springer-Verlag Tokyo)

      Pages: 105-105

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The tissue-engineered vascular graft using bone marrow without culture2005

    • Author(s)
      Hibino N, Shin'oka T, Matsumura G, Ikada Y, Kurosawa H
    • Journal Title

      J Thorac Cardiovasc Surg 129

      Pages: 1064-1070

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Autopericardial patch tracheoplasty for tracheal stenosis after arterial switch operation2005

    • Author(s)
      Takiguchi M, Aoki M, Shin'oka T, Hiramatsu T, Imai Y
    • Journal Title

      J Card Surg 20

      Pages: 244-246

    • Related Report
      2005 Annual Research Report
  • [Journal Article] A successful total cavopulmonary connection conversion 13 years after anatomic repair for tricuspid atresia IIc with severe pulmonary resistance2005

    • Author(s)
      Sugimoto K, Kurosawa H, Shin'oka T, Nagatsu M, Morishima S
    • Journal Title

      J Thorac Cardiovasc Surg 129

      Pages: 684-685

    • Related Report
      2005 Annual Research Report
  • [Journal Article] One-stage intracardiac repair in combination with external stenting of the trachea and right bronchus for tetralogy of Fallot2005

    • Author(s)
      Sakamoto T, Nagase Y, Hasegawa H, Shin'oka T, Tomimatsu H, Kurosawa
    • Journal Title

      J Thorac Cardiovasc Surg 130

      Pages: 1717-1718

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Successful Application of Pulmonary Arterial Patch for Coronary Angioplasty Late after Arterial Switch Operation2005

    • Author(s)
      Kosaka Y, Shin'oka T, Yamazaki K, Kurosawa H
    • Journal Title

      Jpn J Thorac Cardiovasc Surg 53

      Pages: 601-603

    • NAID

      10016903731

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Mid-term clinical results of tissue engineered vascular autografts seeded with autologous bome marrow cells2005

    • Author(s)
      Shin'oka T, Matsumura G, Hibino N, Naito Y, Watanabe M, Konuma T, Sakamoto T, Nagatsu M, Kurosawa
    • Journal Title

      J Thorac Cardiovasc Surg 129

      Pages: 1330-1338

    • Related Report
      2005 Annual Research Report
  • [Journal Article] A successful total cavopulmonary connection conversion 13 years after an anatomic repair for tricuspid atresia IIc with severe pulmonary resistance2005

    • Author(s)
      Sugimoto K, Kurosawa H, Shin'oka T, Nagatsu M, Morishima S, Sakamoto T
    • Journal Title

      J Thorac Cardiovasc Surg 129

      Pages: 684-685

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 臨床応用が実現した再生医療2005

    • Author(s)
      新岡俊治
    • Journal Title

      薬の知識 56

      Pages: 11-12

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Clinical results of tissue-engineered vascular autografts seeded with autologous bone marrow cells2004

    • Author(s)
      Shin' oka T
    • Journal Title

      Nippon Geka Gakkai Zasshi 105,8

      Pages: 459-459

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Establishing Right ventricle - Pulmonary Artery Continuity by Autologous Tissue : An Alternative Approach for Prosthetic Conduit Repair2004

    • Author(s)
      Isomatsu Y, Shin'oka T, Aoki M, Terada M, Takeuchi T, Hoshino S, Takanashi Y, Imai Y, Kurosawa H
    • Journal Title

      Annals of Thoracic Surgery 78

      Pages: 2004-2004

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Clinical results of tissue engineered vascular autografts2004

    • Author(s)
      Shin' oka T
    • Journal Title

      Yonsei Medical Journal 45

      Pages: 73-73

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 骨髄細胞を用いた再生血管移植の臨床例2004

    • Author(s)
      松村剛毅, 新岡俊治
    • Journal Title

      炎症と免疫 12

      Pages: 154-154

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Clinical results of tissue-engineered vascular autografts seeded with autologous bone marrow cells2004

    • Author(s)
      Shin'oka T
    • Journal Title

      Nippon Geka Gakkai Zasshi (Japanese) 105(8)

      Pages: 459-63

    • NAID

      110003890384

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Establishing Right Ventricle- Pulmonary Artery Continuity by Autologous Tissue : An Alternative Approach for Prosthetic Conduit Repair2004

    • Author(s)
      Isomatsu Y, Shin'oka T, Aoki M, Terada M, Takeuchi T, Hoshino S, Takanashi Y, Imai Y, Kurosawa H
    • Journal Title

      Ann Thorac Surg 78

      Pages: 178-180

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 「研究成果報告書概要(欧文)」より2004

    • Author(s)
      Shin'oka T
    • Journal Title

      Yonsei Medical Journal 45

      Pages: 73-74

    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Mid-term clinical results of tissue engineered vascular autografts seeded with autologous bome marrow cells2004

    • Author(s)
      Toshiharu Shin'oka
    • Journal Title

      Yonsei Medical Journal 45

      Pages: 73-74

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 骨髄細胞を使用する再生血管移植の臨床2004

    • Author(s)
      新岡俊治
    • Journal Title

      外科学会雑誌 105

      Pages: 456-463

    • NAID

      10011602257

    • Related Report
      2004 Annual Research Report
  • [Book] Clinical Application of Tissue-Engineered Blood Vessels : Cardiovascular Regeneration Therapies Using Tissue Engineering Approaches2005

    • Author(s)
      GokiMatsumura, Toshiharu Shin'oka
    • Total Pages
      105
    • Publisher
      Springer-Verlag Tokyo
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] Cardiovascular Regeneration Therapies Using Tissue Engineering Approaches2005

    • Author(s)
      Matsumura G, Shin'oka T
    • Total Pages
      238
    • Publisher
      Springer
    • Related Report
      2005 Annual Research Report
  • [Book] Cardiovascular Regeneration Therapies Using Tissue Engineering Approaches2005

    • Author(s)
      Matsumura G, Shin'oka T
    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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