| Project/Area Number |
16591450
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Cerebral neurosurgery
|
|---|
| Research Institution | Sapporo Medical University |
|---|
Principal Investigator |
NONAKA Tadashi Sapporo Medical University, School of Medicine, Assistant Professor, 医学部, 講師 (60180759)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HONMOU Osamu Sapporo Medical University, School of Medicine, Assistant Professor, 医学部, 講師 (90285007)
HOUKIN Kiyohiro Sapporo Medical University, School of Medicine, Professor, 医学部, 教授 (90229146)
|
|---|
| Project Period (FY) |
2004 – 2006
|
| Project Status |
Completed (Fiscal Year 2006)
|
| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥1,500,000 (Direct Cost: ¥1,500,000)
|
|---|
| Keywords | adult / bone marrow / gene / human / neuroprotection / peripheral blood / spiral cord injury / transplantation / gene / human / neuroprotection / peripheral blood / spinal cord injury / transplantation |
|---|
| Research Abstract |
Intravenous injection of mesenchymal stem cells (MSCs) prepared from adult bone marrow (BMSCs) has been reported to ameliorate functional deficits in several CNS diseases in experimental animal models. Although bone marrow was enriched in MSCs, MSC-like multipotent precursor cells (PMSCs) have also been suggested to exist in peripheral blood. These cells will grow to confluency in appropriate culture conditions as flattened fibroblast-like cells. Despite the fact that the stem/precursor cells in peripheral blood is widely used for reconstruction in the hemopoietic system, it remains unclear whether peripheral blood-derived plastic-adherent stem/precursor cells (PMSCs) have potential utility for CNS diseases.
To compare the potential of PMSCs and BMSCs for neural differentiation in vitro, BMSCs and PMSCs were prepared from the adult rat and expanded in culture. Although the growth rate of PMSCs was less than BMSCs, immunocy ochemical and RT-PCR analyses indicated that both MSC types were successfully induced to nestin-positive neurospheres in the presence of EGF and bFGF. After withdrawal of the mitogens, these cells could differentiate into neurofilament-positive neurons or GFAP-positive glia.
To test the hypothesis that treatment with PMSCs may have a therapeutic benefit in spinal cord injury, we compared the efficacy of BMSCs and PMSCs. Rat BMSCs and PMSCs were prepared in culture and injected into the injured spinal cord in the rats. Lesion was assessed 3 weeks after injury using MR imaging and histology. Functional outcome was also assessed. Both BMSCs and PMSCs treated groups had functional improvement compared to the control group. The therapeutic benefits of both MSC-treated groups were similar. These data suggest that PMSCs derived from peripheral blood could be an important cell source of cell therapy for spinal cord injury.
|
