| Project/Area Number |
16591458
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Cerebral neurosurgery
|
|---|
| Research Institution | JUNTENDO UNIVERSITY |
|---|
Principal Investigator |
MIYAJIMA Masakazu JUNTENDO UNIVERSITY, NEUROSURGERY ASSOCIATE, PROFESSOR, 医学部, 助教授 (60200177)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
ARAI Hajime JUNTENDO UNIVERSITY, NEUROSURGERY, PROFESSOR, 医学部, 教授 (70167229)
OOYAMA Kazutaka JUNTENDO UNIVERSITY, NEUROSURGERY, ASISTANT, 医学部, 助手 (20286719)
TAKA Hikari JUNTENDO UNIVERSITY, PROTEOMICS AND BIOMOLECULAR SCIENCE, ASISTANT, 医学部, 助手 (60338374)
村山 季美枝 順天堂大学, 医学部, 助教授 (20053118)
|
|---|
| Project Period (FY) |
2004 – 2005
|
| Project Status |
Completed (Fiscal Year 2005)
|
| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2004: ¥1,500,000 (Direct Cost: ¥1,500,000)
|
|---|
| Keywords | idiopathic normal pressure hydrocephalus / cerebrospinal fluid / proteome analysis / ELISA / LRG / LRG1 / 2D-PAGE / 質量分析 / leucine-rich alpha-2-glycoprotein |
|---|
| Research Abstract |
Objective : The pathogenesis of idiopathic normal pressure hydrocephalus (INPH) is unknown, and the syndrome of INPH remains a diagnostic and therapeutic challenge. The present study investigated the disease-specific protein biomarkers that aid in the diagnosis and treatment of INPH and thus to study the role in the disease process.
Methods : A comparative proteomic analysis was used for clinical screening of cerebrospinal fluid (CSF) proteins in 15 patients with INPH and compared to 12 normal subjects. Furthermore, enzyme linked immunosorbent assay (ELISA) was performed for comparison of CSF proteins between individual INPH patients and controls.
Results : Seven proteins and their isoforms, including leucine-rich _--2-glycoprotein (LRG), _-1-antichymotrypsin, apolipoprotein D, apolipoprotein J, haptoglobin _-1, serum albumin, and _--1-microglobulin/bikunin precursor showed significant changes in CSF of INPH patients compared to controls by proteomic analysis. And significant higher CSF levels of LRG in INPH patients compared to controls were found by ELISA.
Conclusion : These results indicate that there are significant differences in the expression of certain proteins in the CSF of patients with INPH and normal subjects, and the CSF level assay of LRG is useful for the diagnosis and the indication for CSF shunting in patients with INPH.
|
