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Study on the molecular mechanism of secretory granule formation of pituitary hormone

Research Project

Project/Area Number 16591461
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionTeikyo University

Principal Investigator

MATSUNO Akira  Teikyo University, School of Medicine, Professor, 医学部, 教授 (00242058)

Co-Investigator(Kenkyū-buntansha) NAGASHIMA Tadashi  Teikyo University, School of Medicine, Professor, 医学部, 教授 (70217991)
FUJIMAKI Takamitsu  Teikyo University, School of Medicine, Associate Professor, 医学部, 助教授 (80251255)
NAKAGOMI Tadayoshi  Teikyo University, School of Medicine, Professor, 医学部, 教授 (90198052)
TAKEKOSHI Susumu  Tokai University, School of Medicine, Associate Professor, 医学部, 助教授 (70216878)
OSAMURA Yoshiyuki  Tokai University, School of Medicine, Professor, 医学部, 教授 (10100992)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2005: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2004: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsGrowth hormone / pituitary cell / secretory granule / GFP / chromogranin A / secretogranin 3 / carboxypeptidase E / phogrin / rab3B
Research Abstract

In order to investigate, in real time, the transport and secretion of pituitary hormone, we have developed an experimental pituitary cell line, GH3 cell, which has secretory granules of GH linked to EYFP. This stable GH3 cell secretes secretory granules of GH linked to EYFP upon stimulated by Ca^<2+> influx or Ca^2 release from storage. This GH3 cell will be useful for the real-time visualization of the intracellular transport and secretion of GH. Moreover, we have cotransfected rab3B linked to ECYP in this GH3 cell, and have elucidated the role of rab3B in GH secretion. Using the similar cotransfection system, we have elucidated the role of chromogranin A, secretogranin 3, carboxypeptidase E, and phogrin in the mechanism of secretory granule formation in pituitary cell.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (15 results)

All 2005 2004

All Journal Article (15 results)

  • [Journal Article] Establishment of stable GH3 cell line expressing enhanced yellow fluoresce in protein-growth hormone fusion protein2005

    • Author(s)
      Matsuno A et al.
    • Journal Title

      J Histochem Cytochem 53

      Pages: 1177-1180

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Three-dimensional imaging of the intracellular localization of growth hormone and prolactin and their mRNA using nanocrystal (Quantum dot) and confocal laser scanning microscopy techniques2005

    • Author(s)
      Matsuno A et al.
    • Journal Title

      J Histochem Cytochem 53

      Pages: 833-838

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Two- or three- dimensional imagings of simultaneous visualization of rat pituitary hormone and its mRNA : comparison between electron microscopy and confocal laser scanning microscopy with semiconductor nanocrystals (Quantum dots)2005

    • Author(s)
      Matsuno A et al.
    • Journal Title

      Acta Histochem Cytochem 38

      Pages: 253-256

    • NAID

      110003166124

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Histopathological review of silent pituitary somatotroph adenoma2005

    • Author(s)
      Matsuno A et al.
    • Journal Title

      Acta Histochem Cytochem 38

      Pages: 217-221

    • NAID

      110003161321

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Establishment of stable GH3 cell line expressing enhanced yellow fluorescein protein-growth hormone fusion protein2005

    • Author(s)
      Matsuno A, et al.
    • Journal Title

      J Histochem Cytochem 53

      Pages: 1177-1180

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Three-dimensional imaging of the intracellular localization of growth hormone and prolactin and their mRNA using nanocrystal (Quantum dot) and confocal laser scanning microscopy techniques2005

    • Author(s)
      Matsuno A, et al.
    • Journal Title

      J Histochem Cytochem 2005 53

      Pages: 833-838

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Two- or three-dimensional imagings of simultaneous visualization of rat pituitary hormone and its mRNA : comparison between electron microscopy and confocal laser scanning microscopy with semiconductor nanocrystals (Quantum dots)2005

    • Author(s)
      Matsuno A, et al.
    • Journal Title

      Acta Histochem Cytochem 38

      Pages: 253-256

    • NAID

      110003166124

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Histopathological review of silent pituitary somatotroph adenoma2005

    • Author(s)
      Matsuno A, et al.
    • Journal Title

      Acta Histochem Cytochem 38

      Pages: 217-221

    • NAID

      110003161321

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Establishment of stable GH3 cell line expressing enhanced yellow fluorescein protein-growth hormone fusion protein2005

    • Author(s)
      Matsuno A et al.
    • Journal Title

      J Histochem Cytochem 53

      Pages: 1177-1180

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Two- or three- dimensional imagings of simultaneous visualization of rat pituitary hormone and its mRNA : comparison between electron microscopy and confocal laser scanning microscopy with semiconductor nanocrystals(Quantum dots)2005

    • Author(s)
      Matsuno A et al.
    • Journal Title

      Acta Histochem Cytochem 38

      Pages: 253-256

    • NAID

      110003166124

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Secretion of high-molecular-weight adrenocorticotropic hormone from a pituitary adenoma in a patient without Cushing stigmata2004

    • Author(s)
      Matsuno A, et al.
    • Journal Title

      J Neurosurg 101

      Pages: 880-883

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A novel strategy for growth suppression of glioma cells : targeting microtubules2004

    • Author(s)
      Matsuno A et al.
    • Journal Title

      Acta Histochem Cytochem 37

      Pages: 153-158

    • NAID

      110003144376

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Specific gene suppression using antisense strategy for growth suppression of glioma2004

    • Author(s)
      Matsuno A et al.
    • Journal Title

      Med Electron Microse 37

      Pages: 158-161

    • NAID

      10014171108

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Secretion of high-molecular-weight adrenocorticotropic hormone from a pituitary adenoma in a patient without Cushing stigmata2004

    • Author(s)
      Matsuno A et al.
    • Journal Title

      J Neurosurg 101

      Pages: 880-883

    • Related Report
      2004 Annual Research Report
  • [Journal Article] In vivo growth suppression of rat C6 glioma transplanted in rat brain using antisense oligonucleotide for microtubule-associated protein 1A messenger ribonucleic acid2004

    • Author(s)
      Matsuno A et al.
    • Journal Title

      Brit J Neurosurg 18

      Pages: 343-346

    • Related Report
      2004 Annual Research Report

URL: 

Published: 2004-04-01   Modified: 2016-04-21  

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