| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2004: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Research Abstract |
Objective. To compare the properties of human mesenchymal stem cells (MSCs) isolated from bone marrow, synovium, periosteum, skeletal muscle, and adipose tissue.
Methods. Human mesenchymal tissues were harvested from 8 donors during the knee operations for the ligament injury. After collagenase digestion or gradient density separation, nucleated cells were plated at a proper density so that they expanded at maximum rate without colony-to-colony contact. Yield, expandability, differentiation potential, and epitope profile were compared among MSCs from bone marrow, synovium, periosteum, skeletal muscle, and adipose tissue.
Results. Colony number per nucleated cells was lower and the cell number per colony was higher in the bone marrow than the other mesenchymal tissues. The cells were replated at low density every 14 days and bone marrow-, synovium-, and periosteum- derived cells retained their proliferation ability even at Passage 10. For chondrogenesis, the cells were pelleted and cultured in vitro, showing pellets from bone marrow-, synovium-, and periosteum- derived cells were larger and stained more extensively for cartilage matrix. Synovium-derived cells, in particular, had the greatest ability for chondrogenesis. For adipogenesis, Oil Red-O positive colony rate was higher in synovium- and adipose- derived cells. For osteogenesis, Alizarin Red positive colony rate was higher in bone marrow-, syovium-, and periosteum- derived cells. For epitope profile, 15 surface antigens were measured and the majority seemed similar irrespective of cell source.
Conclusion. We revealed significant differences in the properties dependent on cell sources beyond donor and experimental variation. Superiority of synovium as cell source was demonstrated.
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