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Research concerning articular cartilage repair using novel growth factor

Research Project

Project/Area Number 16591484
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionShiga University of Medical Science

Principal Investigator

MATSUSUE Yoshitaka  Shiga University of Medical Science, Department of Orthopaedic Surgery, Professor, 医学部, 教授 (30209548)

Co-Investigator(Kenkyū-buntansha) IMAI Shinji  Shiga University of Medical Science, Department of Physical medicine and Rehabilitation, Associate professor, 医学部, 助教授 (90283556)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2004: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordsarticular cartilage repair / growth factor / DDS / 家兎 / ポリ乳酸
Research Abstract

AG-041R is a cholecystokinin-B/gastrin receptor antagonist, which was originally developed to treat gastric ulcers. In a preclinical toxicological study on rats, oral administration of a high dose of AG-041R was found to stimulate systemic cartilage hyperplasia, including the articular cartilage.
1 ; The first aim of this study is to develop the way of local administration for articular cartilage.
PDLLA microspheres containing AG-041R were prepared by the solvent-evaporation method. Many kinds of microspheres was made by mixing various amounts of AG-01R and various kinds of PL(G)A. We indeed implanted the collagen containing microsphere into the 5 mm-diameter full-thickness articular cartilage defect of rabbit patellar groove. After certain weeks, we sacrificed the rabbit and measure the AG-041R content in synovial fluid and reparative tissue by HPLC. We could confirm that PLA0020 microsphere containing 40% AG-04R remained in reparative tissue for 4 weeks, which we thought ideal periods.
2 ; The second aim is to verify the effectiveness ofAG-041R for articular cartilage repair and to examine it histologically.
We made the same cartilage defect as above in both knee. Collagen gel mixed with 0.5 or 2.5mg PLA microspheres containing 40% were implanted into the left knee, as experimental group, and collagen gel mixed with same volume of just PLA microsphere, as control group. 4, 8, 12 weeks after implantation, reparative tissues were examined histologically by Toluidin Blue and immunofistochemistoly of type I and II collagen. 12 weeks after implantation, the reparative tissues of experimental group implanted with 2.5mg PLA microspheres containing 40 % were well repaired and the tissue were confined type II collagen positive and type I collagen negative.
As future direction, we planed the more detailed histologically examination by BrdU stain for proliferating cell and TUNEL stain for apoptosis.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (29 results)

All 2006 2005 2004

All Journal Article (26 results) Book (3 results)

  • [Journal Article] Mosaicplastyによる関節軟骨修復2006

    • Author(s)
      松末吉隆
    • Journal Title

      医学の歩み 216(6)

      Pages: 447-450

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Mosaicplastyによる軟骨修復の臨床2006

    • Author(s)
      松末吉隆
    • Journal Title

      関節外科 25(4月増刊号)

      Pages: 75-82

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Articular cartilage repair with Mosaicplasty2006

    • Author(s)
      Yoshitaka Matsusue
    • Journal Title

      Igakuno-Ayumi 216(6)

      Pages: 447-450

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Repair of Articular cartilage repair with Mosaicplasty2006

    • Author(s)
      Yoshitaka Matsusue
    • Journal Title

      Journal of Joint Surgery 25 suppl

      Pages: 75-82

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 膝蓋大腿関節障害の診断と治療2005

    • Author(s)
      松末吉隆
    • Journal Title

      Monthly Book Orthopaedics 18(5)

      Pages: 134-141

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Posttraumatic recurrent dislocation of extensor pollicis brevis tendon over the matacarpophalangeal joint.2005

    • Author(s)
      Shinji Imai, Yohitaka Matsusue
    • Journal Title

      The Journal of Hand Surgery 30A

      Pages: 147-150

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Diagnosis and therapy of patello-femoral disorders2005

    • Author(s)
      Yoshitaka Matsusue
    • Journal Title

      Monthly Book Orthopaedics 18(5)

      Pages: 134-141

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Posttraumatic recurrent dislocation of extensor pollicis brevis tendon over the matacarpophalangeal joint2005

    • Author(s)
      Shinji Imai, Yoshitaka Matsusue
    • Journal Title

      The Journal of Hand Surgery 30A

      Pages: 147-150

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The complete process of bioresorption and bone replacement using devices made of forged composites of raw hydroxyapatite particles/poly L-lactide (F-u-HA/PLLA)2005

    • Author(s)
      Shikinami Y, Matsusue Y, Nakamura T
    • Journal Title

      Biomaterials 26

      Pages: 5542-5551

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Posttraumatic Recurrent Dislocation of Extensor Pollicis Brevis Tendon over the Metacarpophalangeal Joint2005

    • Author(s)
      Imai S, Kikuchi K, Matsusue Y
    • Journal Title

      J Hand Surg 30A

      Pages: 147-150

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Nociceptive pathway and pathology of low back pain2005

    • Author(s)
      Imai S
    • Journal Title

      Current Rheumatology Reviews 1

      Pages: 167-176

    • Related Report
      2005 Annual Research Report
  • [Journal Article] PLLAとHA複合体を用いた骨欠損に対する治療2004

    • Author(s)
      松末吉隆
    • Journal Title

      関節外科 23(2)

      Pages: 238-244

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 生体内吸収性材料の現状と今後の展望2004

    • Author(s)
      松末吉隆
    • Journal Title

      リウマチ科 32(1)

      Pages: 1-11

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 生体内吸収性ポリ乳酸製材料2004

    • Author(s)
      松末吉隆
    • Journal Title

      骨・関節・靭帯 17(11)

      Pages: 1243-1251

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 膝骨壊死に対する骨軟骨移植法2004

    • Author(s)
      松末吉隆
    • Journal Title

      日本整形外科学会雑誌 78(11)

      Pages: 854-860

    • NAID

      10014246942

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Spontaneous Rupture of the Flexor Digitorum Profundus and Superficialis of the Index Finger and the Flexor Policis Longus without Labor-Associated Tendon Loading2004

    • Author(s)
      Shinji Imai, Yoshitaka Matsusue
    • Journal Title

      The Journal of Hand Surgery 29A(4)

      Pages: 587-590

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The therapy for bone defect using PLLA and HA composite2004

    • Author(s)
      Yoshitaka Matsusue
    • Journal Title

      Journal of Joint Surgery 23(2)

      Pages: 238-244

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The present condition and future direction of bioabsorbable materials2004

    • Author(s)
      Yoshitaka Matsusue
    • Journal Title

      Rheumatology 32(1)

      Pages: 1-11

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Bioabsorbable PLA materials2004

    • Author(s)
      Yoshitaka Matsusue
    • Journal Title

      The journal of musculoskeletal system 17(11)

      Pages: 1243-1251

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Osteochondral transplantation for osteonecrosis of the knee2004

    • Author(s)
      Yoshitaka Matsusue
    • Journal Title

      Journal of Japanese Orthopaedic Association 78(11)

      Pages: 854-860

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Spontaneous Rupture of the Flexor Digitorum Profundus and Superficialis of the Index Finger and the Flexor Policis Longus without Labor-Associated Tendon Loading2004

    • Author(s)
      Shinji Imai, Yoshitaka Matsusue
    • Journal Title

      The Journal of Hand Surgery 29(A)

      Pages: 587-590

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Osteochondral grafting for cartilage defects in the patellar grooves of bilateral knee joints.2004

    • Author(s)
      Nakagawa Y, Matsusue Y, Suzuki T, et al.
    • Journal Title

      Arthroscopy 20(Suppl 2)

      Pages: 32-38

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Association of CYP17 with HLA-B27-negative seronegative spondyloarthropathy in Japanese males.2004

    • Author(s)
      Mori K, Kizawa H, Matsusue Y et al.
    • Journal Title

      Am J Med Genet 30(2)

      Pages: 169-171

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Intramedullary spinal cord metastasis of ovarian tumor.2004

    • Author(s)
      Isoya E, Saruhash Y, Matsusue Y et al.
    • Journal Title

      Spinal Cord 42(8)

      Pages: 485-487

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Type-selective muscular degeneration promotes infiltrative growth of intramuscular lipoma.2004

    • Author(s)
      Mori K, Chano T, Matsusue Y et al.
    • Journal Title

      BMC Musculoskelet Disord 5(1)

      Pages: 20-20

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Expression of macrophage inflammatory protein-1alpha in Schwann cell tumors.2004

    • Author(s)
      Mori K, Chano T, Matsusue Y et al.
    • Journal Title

      Neuropathology 24(2)

      Pages: 131-135

    • Related Report
      2004 Annual Research Report
  • [Book] ナースの整形外科学2005

    • Author(s)
      松末吉隆
    • Publisher
      中外医学社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] 整形外科学・外傷学2005

    • Author(s)
      松末吉隆
    • Publisher
      文光堂
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] 今日の整形外科治療指針 第5版2004

    • Author(s)
      松末吉隆
    • Publisher
      医学書院
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary

URL: 

Published: 2004-04-01   Modified: 2016-04-21  

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