| Project/Area Number |
16591498
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | University of Miyazaki |
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Principal Investigator |
CHOSA Etsuo University of Miyazaki, Faculty of Medicine, Orthopaedic Surgery, Professor (00236837)
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| Co-Investigator(Kenkyū-buntansha) |
HAMADA hiroaki University of Miyazaki, Faculty of Medicine, Orthopaedic Surgery, Professor (40363593)
SAKAMOTO takero University of Miyazaki, Faculty of Medicine, Orthopaedic Surgery, Assistant (50325742)
KITAMURA Kazuo University of Miyazaki, Faculty of Medicine, Internal Medicine, Professor (50204912)
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| Project Period (FY) |
2004 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,950,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥150,000)
Fiscal Year 2007: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2006: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2005: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2004: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | Adrenomedullin / PAMP(preadrenomedullin N-terminal peptide) / Arthritis / Chondrocvte / Anti-inflammatoryeffects / Synovia / Apoptosis / リウマチ性関節炎 / 滑膜細胞培養 / 軟骨細胞分化 |
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| Research Abstract |
The plasma adrenomedullin and PAMP level was about twice higher than the normal level in the patient with rheumatic arthritis, in addition, adrenomedullin equal with the concentration which was included in the plasma and in the joint fluid. Synovia has higher amount of adrenomedullin than ligamentous tissues and meniscus in the joint tissues in the patient with rheumatic arthritis. These evidences lead to us the possibility of adrenomedullin acting chiefly as a hormone in vivo and suggestion of the fashion of the adrenomedullin activity mainly in synovia.
In the synovia with the rheumatic arthritis, the gene expression of adrenomedullin and PAMP is confirmed, and these were composed secretion in the cultured the synovia. Moreover, the secretion of IL - 6 was significantly inhibited in addition with adrenomedullin. It was clarified that there was a significant anti-inflammatory effect in the role of adrenomedullin in the inflammatory synovium.
When adrenomedullin was administered by using an anti-inflammatory effect in arthritis, in using the rabbit adjuvant arthritis model, adrenomedullin caused the reduce of the swelling, decreasing the number of the lymphocytes and promotion of fibrous change in five days after it had treated with injection into a swollen joint. Therefore, it was proven that adrenomedullin had a role as the regulator for arthritis in rheumatic arthritis.
In addition normal joint chondrocytes voluminously stored adrenomedullin in the granules in its cytoplasm. In the primary cultured chondrocytes, adrenomedullin induced cell apoptosis and necrosis as a dose depended manner, and the efficiency was clarified for the cell proliferation as a local regulator in the arthritis.
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