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Cartilage regeneration using high performance extra-cellular matrix and development of noninvasive mechanical property evaluation

Research Project

Project/Area Number 16591512
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionTokai University School of Medicine

Principal Investigator

SATO Masato  Tokai Univ., School of Medicine, Assistant Professor, 医学部, 講師 (10056335)

Co-Investigator(Kenkyū-buntansha) MOCHIDA Joji  Tokai Univ., School of Medicine, Professor, 医学部, 教授 (50174347)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2005: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2004: ¥2,700,000 (Direct Cost: ¥2,700,000)
KeywordsTissue engineering / Extra-cellular matrix / Growth factor / Scaffold / Biomaterial / Heparin / Noninvasive evaluation / Photoacoustic measurement / 非侵襲的力学特性評価
Research Abstract

The specific aim of our investigation is to study the potential use of a collagen/heparin-carrying polystyrene (HCPS) composite extracellular matrix for articular cartilage tissue engineering. In this study we created a high- performance extracellular matrix (HpECM) scaffold in order to create an optimal extracellular environment using an HCPS we originally developed, and an atelocollagen honeycomb-shaped scaffold with a membrane seal (ACHMS-scaffold). This scaffold was coated with HCPS to prepare the HpECM-scaffold to enable aggregation of heparin-binding growth factors such as FGF-2 and TGFβwithin the scaffold. Three-dimensional culture of rabbit articular chondrocytes within the HpECM-scaffold and subsequent preparation of a tissue-engineered cartilage were investigated using FGF-2 and TGFβ. The results showed remarkably higher cell proliferative activity within the HpECM-scaffold with FGF-2 and the effect of differentiation induction within the HpECM- scaffold with TGFβ. It was thought that both FGF-2 and TGFβ, which have binding sites to heparin, were stably immobilized in the HpEMC- scaffold since HCPS generated an extracellular environment similar to that of heparan sulfate proteoglycan within the scaffold. Thus, the effects of matricrine and juxtacrine were displayed, and their respective activities were maintained in a long-term effect. At the same time, endogenous growth factor, cytokines, and other functional substances might also be retained within the HpECM-scaffold.
Tissue engineered cartilage using HpECM was evaluated according to the photoacoustic measurement method. As a result, the cartilage increased dynamics strength with the passage of time and HpECM had the tendency to obtain the mechanical characteristic from the early stage of cultivation.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (9 results)

All 2005 2004

All Journal Article (7 results) Book (2 results)

  • [Journal Article] Usefulness of photoacoustic measurements for evaluation of biomechanical properties of tissue-engineered cartilage2005

    • Author(s)
      Ishihara M et al.
    • Journal Title

      Tissue Engineering 11

      Pages: 1234-43

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] 軟骨再生医療の評価に用いる光音響法の開発2005

    • Author(s)
      石原美弥 他
    • Journal Title

      日本レーザー医学会誌 26

      Pages: 53-59

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Usefulness of photoacoustic measurements for evaluation of biomechanical properties of tissue-engineered cartilage.2005

    • Author(s)
      Ishihara M, Sato M, Sato S, Kikuchi T, Mochida J, Kikuchi M.
    • Journal Title

      Tissue Engineering 11(7-8)

      Pages: 1234-1243

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Usefulness of photoacoustic measurements for evaluation of the biomechanical properties of tissue-engineered cartilage2005

    • Author(s)
      Ishihara M et al.
    • Journal Title

      Tissue Engineering (In press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Tissue Engineering of Articular Cartilage using an allograft of cultured chondrocytes in a membrane-sealed atelocollagen honeycomb-shaped scaffold(ACHMS-scaffold)2005

    • Author(s)
      Masuoka K et al.
    • Journal Title

      J.Biomed.Mater.Res. (In press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Characterization of intervertebral disc--disc cells and pericellular microenvironment2004

    • Author(s)
      Sato M et al.
    • Journal Title

      Clinical Calcium 14

      Pages: 64-69

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Osteogenic potential of human adipose tissue-derived stromal cells as an alternative stem cell source2004

    • Author(s)
      Hattori H et al.
    • Journal Title

      Cells Tissues Organs 178

      Pages: 2-12

    • Related Report
      2004 Annual Research Report
  • [Book] 遺伝子医学MOOK1 再生医療へのブレイクスルー : 再生医療を具現化するための基盤技術としての光計測・評価技術2005

    • Author(s)
      石原美弥 他
    • Publisher
      メディカルドゥ
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] 遺伝子医学MOOK1 再生医療へのブレイクスルー:再生医療を具現化するための基盤技術としての光計測・評価技術2005

    • Author(s)
      石原美弥 他
    • Publisher
      メディカルドゥ
    • Related Report
      2005 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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