| Project/Area Number |
16591533
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
AIMI Yoshinari Shiga University of Medical Science, Department of Anatomy, Assistant Professor, 医学部, 助手 (20231756)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUO Akinori Molecular Neuroscience Research Center, Assistant Professor, 分子神経科学研究センター, 助手 (20324585)
YASUHARA Osamu Molecular Neuroscience Research Center, Associate Professor, 分子神経科学研究センター, 助教授 (80239772)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2004: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | pChAT / cholinergic neuron / nociception / dorsal root ganglion / immunohistochemistry |
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| Research Abstract |
The purpose of this study was to investigate a possible involvement of acetylcholine in nociception by using pChAT as a marker of cholinergic nervous system. In the first year, we demonstrated an existence of pChAT neurons in the dorsal root ganglion and central and peripheral projection of fibers from these pChAT neurons. Co-localization of pChAT and other substances including SP, CGRP, NOS, VR1 was also demonstrated. As a basic experiment for further physiological chemical stimulation study of these pChAT neurons, we examined intercellular translocation of pChAT protein. In the second year, we investigated a relationship between pChAT positive terminals and acetylcholine receptors. Although we have tried several antibodies to stain acetylcholine receptors in the spinal cord, so far, the results of the staining were on unsatisfactory level. Furthermore, we found that it was difficult to stain pChAT nerve endings whereas cell bodies and proximal fibers of the pChAT neurons were stained well. To solve these difficulties, we are checking various conditions of pChAT immunostaining. Another goal of this study was to observe the direct evidence of acetylcholine release from pChAT neurons. As a basic study for the goal, we measured a content of acetylcholine in the DRG. We are studying acetylcholine release from cultured pChAT neurons using micro-HPLC system with enzyme column.
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