| Project/Area Number |
16591536
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Osaka University |
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Principal Investigator |
UCHIYAMA Akinori Osaka University, Hospital, Assistant professor, 医学部附属病院, 助手 (00324856)
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| Co-Investigator(Kenkyū-buntansha) |
FUJINO Yuji Osaka University, Graduate School of Medicine, Lecturer, 医学系研究科, 講師 (50252672)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2004: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | mechanical ventilation / diaphragm / sepsis / partial ventilatory assist / pressure support ventilation / rat / ventilator-induced diaphragmatic dysfunction / endotoxin / 補助換 / 敗血症モデル / 補助換気 / 横隔膜筋力 / LPS / 間歇的強制換気 / 呼吸筋筋力 / 筋障害 |
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| Research Abstract |
In sepsis, ventilatory muscle injury is a cause of respiratory failure. While mechanical ventilation can protect against injury, it may also induce diaphragmatic dysfunction. With pressure support ventilation (PSV), physicians can lighten the work of breathing. Our hypothesis was that partial ventilatory assistance settings during PSV affect the extent of diaphragmatic dysfunction in sepsis. We tested how ventilatory assistance affects lipopolysaccharide (LPS)-induced diaphragmatic dysfunction. Male Sprague-Dawley rats were injected with LPS and subjected,30 min later, to one of four ventilatory settings for 300 min (n=10×4=40:CPAP+PSV mode with PSV at 0,5,10(S:slow pressure rising) and 10 cmH2O(F:fast pressure rising). Control animals (n=10) received saline instead of LPS and underwent 300 min of CPAP 5 cmH2O without PSV. We set a PEEP of 5 cmH2O. We examined diaphragmatic function using the isolated diaphragmatic strip method. While the titanic tension levels in PSVO,PSV10S and PSV10F were lower than in the Control, no significant differences from the Control were observed in PSV5. The tension values of PSV5 were the highest after the LPS administration. Varying levels of PSV affects LPS-induced diaphragmatic dysfunction. Instead of a high level of PSV, a certain level of PSV slows LPS-induced diaphragmatic dysfunction.
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