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Molecular mechanism of acute and chronic pain

Research Project

Project/Area Number 16591543
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionOKAYAMA UNIVERSITY

Principal Investigator

MIZOBUCHI Satoshi  Okayama University, Hospital of Medicine and Dentistry, Lecturer, 医学部・歯学部附属病院, 講師 (70311800)

Co-Investigator(Kenkyū-buntansha) YOKOYAMA Masataka  Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Associate Professor, 大学院医歯薬学総合研究科, 助教授 (20158380)
TAKAHASHI Toru  Okayama University, Hospital of Medicine and Dentistry, Lecturer, 医学部・歯学部附属病院, 講師 (40252952)
NAKATSUKA Hideki  Okayama University, Hospital of Medicine and Dentistry, Lecturer, 医学部・歯学部附属病院, 講師 (70263580)
NINOMIYA Yoshifumi  Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Professor, 大学院医歯薬学総合研究科, 教授 (70126241)
MORITA Kiyoshi  Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Professor, 大学院医歯薬学総合研究科, 教授 (40108171)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2004: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsmolecular mechanisms / acute pain / chronic pain / BDNF / spinal cord / 疼痛メカニズム / 難治性疼痛 / 遺伝子治療
Research Abstract

To investigate molecular mechanisms of acute and chronic pain, we achieved two main projects in 2004-2005. First, to clarify the influence of acute pain under chronic neuropathic pain states, we examined the expression of brain-derived neurotrophic factor (BDNF) and calcitonin gene-related peptide (CGRP) in the spinal cord of rats with L5 spinal nerve ligation (SNL)-induced neuropathy and investigated the expression of c-Fos in the spinal cord after injection of formalin in the hindpaw of rats with SNL. Four weeks after SNL, the withdrawal threshold was significantly lower in the SNL group. In the SNL group, expression of BDNF in the L4 and L5 superficial dorsal horn was significantly decreased compared to that in the sham group. CGRP protein in the L5 but not in the L4 dorsal horn was significantly decreased compared to that in the sham group. After formalin injection, spontaneous pain responses in the SNL group were significantly decreased. Immunolabeling for c-Fos was significantly … More decreased in the L4 and L5 dorsal horn in the SNL. In this study, these results indicate that decreased neuronal activity in the spinal cord in response to inflammatory pain may be important for altering the perception of acute pain and decreased BDNF expression in response to SNL-induced neuropathy may be involved in this alteration. Second, we assessed multiple BDNF transcripts semiquantitatively by reverse transcription polymerase chain reaction (RT-PCR) technique using exon-specific primers because BDNF is one of the most noteworthy substances in pain research. In naive rats, there was no significant difference between in ipsilateral and contralateral dosal root ganglions(DRGs) as for any exons expression. In L5SNL rats, exon V expression elevated significantly in ipsilateral L4DRG compared to contralateral, which demonstrates increased expression of total BDNF mRNA. In ipsilateral side, a significant increase in exon I expression was observed. In L5 DRGs of L5SNL rats, there was no significant difference in exon V expression. Among four 5' exons, only the ipsilateral exon I expression elevated significantly compared to contralateral. Our results showed that most of BDNF mRNA elevated in ipsilateral L4 DRG of L5SNL model is exon I mRNA. This indicates that exon I mRNA plays important role in modulating neuronal transmission in neuropathic pain. Less

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (4 results)

All 2004

All Journal Article (4 results)

  • [Journal Article] The effects of pretreatment with lidocaine or bupivacaine on the spatial and temporal expression of c真Fos protein in the spinal cord caused by plantar incision in the rat.2004

    • Author(s)
      Xiaohui Sun, Masataka Yokoyama, Satoshi Mizobuchi, Rhuji Kaku, Hideki Nakatsuka, Toru Takahashi, Kiyoshi Morita
    • Journal Title

      Anesthesia & Analgesia 98

      Pages: 1093-8

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Comparison of percutaneous electrical nerve stimulation with transcutaneous electrical nerve stimulation for long-term pain relief in patients with chronic low back pain.2004

    • Author(s)
      Masataka Yokoyama, Xiaohui Sun, Satoru Oku, Naoyuki Taga, Kenji Sato, Satoshi Mizobuchi, Toru Takahashi, Kiyoshi Morita
    • Journal Title

      Anesthesia & Analgesia 98

      Pages: 1552-6

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The effects of pretreatment with lidocaine or bupivacaine on the spatial and temporal expression of c-Fos protein in the spinal cord caused by plantar incision in the rat.2004

    • Author(s)
      Xiaohui Sun, Masataka Yokoyama, Satoshi Mizobuchi, Rhuji Kaku, Hideki Nakatsuka, Toru Takahashi, Kiyoshi Morita
    • Journal Title

      Anesthesia & Analgesia 98

      Pages: 1093-1098

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The effects of pretreatment with lidocaine or bupivacaine on the spatial and temporal expression of c-Fas protein in the spinal cord caused by plantar incision in the rat2004

    • Author(s)
      Sun X, Yokoyama M, Mizobuchi S, Kaku R, Nakatsuka H, Takahashi T, Morita K.
    • Journal Title

      Anesthesia & Analgesia 98

      Pages: 1093-1098

    • Related Report
      2004 Annual Research Report

URL: 

Published: 2004-04-01   Modified: 2016-04-21  

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