| Project/Area Number |
16591548
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
NAGATA Taro Kyushu University, Reseach Associate, 大学院・医学研究院, 助手 (70284510)
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| Co-Investigator(Kenkyū-buntansha) |
NODA Yukiko Kyushu University, Kyushu University Hospital, Reseach Associate, 大学病院, 助手 (10404021)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2004: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | dendritc spines / small GTPases / dendritic spine / 麻酔薬 / シナプス / RhoファミリーG蛋白質 |
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| Research Abstract |
The progress of anesthesiology has made many invasive surgical operations possible. However, little is known about the molecular mechanism of anesthetized state. We hypothesized that inhibition of actin dynamics occured during general anesthesia at brain synapses. In the present study, we investigated the influence of anesthetics on actin-based motility.
NIH 3T3 cells and Swiss 3T3 cells showed actin-based ruffling at their periphery after the addition of PMA (phorbol 12-myristate 13-acetate) to the medium. Some cultures were exposed to anesthetics (propofol) before treatment of PMA, but actin rearrangement was not blocked. The same results were obtained when PDGF were added instead of PMA. We could not see the effect of anesthetics on actin rearrangement.
The small GTPases Rac1 and Cdc42 are members of Rho subfamily of the Ras-related GTP-binding proteins and participates in cytoskeltal reorganization. We examined to detect the GTP-bound, active Rac/Cdc42 in neuronal cells cultured with or without the stimulation. However, active Rac/Cdc42 was difficult to detect, because of their high GTPase activity. Therefore we tried to detect Rap1 small GTPase, which also considered to be related to morphogenesis of dendritic spines. NMDA, AMPA, and their receptor antagonists did not influence Rap1 activity.
It is considered that Rap1 activity is not affected by stimulation or inhibition of AMPA or NMDA receptors.
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