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The role of galectin-3 in human renal cell cancer cells

Research Project

Project/Area Number 16591599
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionThe University of Tokushima

Principal Investigator

KANAYAMA Hiro-omi  The University of Tokushima, Institute of Health Biosciences, Professer, 大学院・ヘルスバイオサイエンス研究部, 教授 (10214446)

Co-Investigator(Kenkyū-buntansha) FUKUMORI Tomoharu  The University of Tokushima, Institute of Health Biosciences, Lecturer, 大学院・ヘルスバイオサイエンス研究部, 講師 (10314874)
NISHITANI Masaaki  The University of Tokushima, Institute of Health Biosciences, Associate Professer, 大学院・ヘルスバイオサイエンス研究部, 助教授 (40304521)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2005: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2004: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordsrenal cell carcinoma / Apoptosis / Galectin-3 / CD8 positive T cells / ガレクチン-3 / CTL
Research Abstract

Galectin-3 is a member of the galectin gene family that is expressed at elevated levels in a variety of neoplastic cell types and has been associated with cell growth, cellular adhesion process, cell proliferation, transformation, metastasis, and apoptosis. We studied the expression of galecin-3 in renal cell carcinoma cell lines and human renal cell carcinoma tissues by RT-PCR and western blot analysis, and investigated the clinical significance and the possibility as a tumor marker. We also investigated its effect on T cell or NK cell activation. The expression of galectin-3 was identified in every renal cell carcinoma cell line we employed and was significantly higher in human renal cell carcinoma tissues than normal kidney tissues. Especially in the case with metastasis, the expression ratio was significantly high (p=0.035), which suggested that ratio could become prognostic factor of metastasis. We extracted CD8 positive T cells from human renal cell carcinoma tissues and investigated the effect of galectin-3 induced apoptosis in the cells by flow cytometry. Galectin-3 significantly induced apoptosis (p=0.039) by binding to the CD8 positive T cells, from which we concluded that galectin-3 might be involved in what is called ‘escape' from immune system of renal cell carcinoma. We have also constructed mice RCC models and metastasis models by employing Renca cells (positive and negative galectin-3 expression) and investigated anti-angiogenesis and anti-tumor effect. These results will be published shortly. To clarify the role of galectin-3 in angiogenesis or apoptosis may lead to understanding mechanism of resistance of renal cell carcinoma to various kinds of therapies. In future we plan to expand these achievements into gene therapy for renal cell carcinoma.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (32 results)

All 2006 2005 2004 Other

All Journal Article (32 results)

  • [Journal Article] Garectin-3 regulates mtochondrial stability and anti-apoptosis function in response to anti-cancer drugs in prostate cancer2006

    • Author(s)
      Fukumori T
    • Journal Title

      Cancer Research 66

      Pages: 3114-3119

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Garectin-3 regulates mitochondrial stability and anti-apoptosis function in response to anti-cancer drugs in prostate cancer2006

    • Author(s)
      Fukumori T
    • Journal Title

      Cancer Research 66

      Pages: 3114-3119

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Expression of angiopoietin-1 and -2, and its clinical significance in human bladder cancer2005

    • Author(s)
      Oka N
    • Journal Title

      BJU international 95

      Pages: 660-663

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] cDNA microarray analysis assists in diagnosis of malignant intrarenal phenochromocytoma originally masquerading as a renal cell carcinoma.2005

    • Author(s)
      Takahashi M
    • Journal Title

      J Med Genet 42・8

      Pages: 48-48

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] a molecular classification of papillary renal cell carsinoma.2005

    • Author(s)
      Yang XJ
    • Journal Title

      Cancer Research 65・13

      Pages: 5628-2637

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Role of the vitamin D receptor in FGF23 action on phosphate metabolism.2005

    • Author(s)
      Inoue Y
    • Journal Title

      Biochem J 390

      Pages: 5628-5637

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Altered expression of members of the IGF-axis in clear cell renal cell carsinoma.2005

    • Author(s)
      Takahashi M
    • Journal Title

      Int J Oncology 26・4

      Pages: 923-931

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Nitrite-derived nitric oxide formation following ischemia-reperfusion injury in kidney2005

    • Author(s)
      Okamoto M
    • Journal Title

      American Lournal of Physiology, Renal Physiology 288・1

      Pages: 182-187

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Implication of galectin-3 in Wnt signaling2005

    • Author(s)
      Shimura T
    • Journal Title

      Cancer Research 65

      Pages: 3535-3537

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Galectin-3 inhibits tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis by activating Akt in human bladder carcinoma cells.2005

    • Author(s)
      Oka N
    • Journal Title

      Cancer Research 65

      Pages: 7565-7553

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] a molecular classification of papillary renal cell carcinoma2005

    • Author(s)
      Yang XJ
    • Journal Title

      Cancer Research 65・13

      Pages: 5628-5637

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Role of the vitamin D receptor in FGF23 action on phosphate metabolism2005

    • Author(s)
      Inoue Y
    • Journal Title

      Biochem J 390

      Pages: 5628-5637

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Altered expression of members of the IGF-axis in clear cell renal cell carsinoma2005

    • Author(s)
      Takahashi M
    • Journal Title

      Int J Oncology 26・4

      Pages: 923-931

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Nitrite-derived nitric oxide formation following ischemia-reperfusion injury in kidney2005

    • Author(s)
      Okamoto M
    • Journal Title

      American Journal of Physiology, Renal Physiology 288・1

      Pages: 182-187

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Galectin-3 inhibits tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis by activating Akt in human bladder carcinoma cells2005

    • Author(s)
      Oka N
    • Journal Title

      Cancer Research 65

      Pages: 7546-7553

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Nuclear Export of Phosphorylated Galectin-3 Regulates its Anti-Apoptotic Activity in Response to Chemotherapeutic Drugs2004

    • Author(s)
      Takenaka Y
    • Journal Title

      Molecular and Cellular Biology 24

      Pages: 4395-4406

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Endogenenous galectin-3 determines the routing of CD95 apoptotic signaling pathways2004

    • Author(s)
      Fukumori T
    • Journal Title

      Cancer Research 64

      Pages: 33766-3379

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Infrequent Alteration in the p53R2 Gene in Human Transitional Cell Carcinoma of the Urinary Tract2004

    • Author(s)
      Hayashi H
    • Journal Title

      Pathobiology 71

      Pages: 103-106

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Expression of Alpha-Methylacy-CoA Racemase in Papillary Renal Cell Carcinoma2004

    • Author(s)
      Tretiakova MS
    • Journal Title

      Am J Surg Pathol 28・1

      Pages: 69-76

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Gene Expression Profiling of Early-and Late-Relapse Nonseminomatous Gerrm Cell Tumor and Primitive Neuroectoderma tumor of Testis2004

    • Author(s)
      Sugimura J
    • Journal Title

      Clinical Cancer Research 10

      Pages: 2368-2378

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Robust Classification of Renal Cell Carcinoma Based on Gene Expression Data and Predicted Cytogenetic Profiles2004

    • Author(s)
      Furga KA
    • Journal Title

      Cancer Research 64

      Pages: 4117-4121

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Characteristics of Patients with Prostate Cancer Who Have Inrtially been Treated by Hormene Therapy in Japan : J-CaP Surveillance2004

    • Author(s)
      Akaza H
    • Journal Title

      Japanese Journal of Clinical Oncology 34・6

      Pages: 329-336

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Endogenous galectin-3 determines the routing of CD95 apoptotic signaling pathways2004

    • Author(s)
      Fukumori T
    • Journal Title

      Cancer Research 64

      Pages: 3376-3379

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Expression of Alpha-Methylacy-CoA Racemase in Papillary Renal Cell Carcinoma2004

    • Author(s)
      Tretiakova MS
    • Journal Title

      Clinical Cancer Research 10

      Pages: 2368-2378

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Robust Classification of Renal Cell Carcinoma Based on Gene Expression Data and Predicted Cytogenetic Profiles2004

    • Author(s)
      Furge KA
    • Journal Title

      Cancer Research 64

      Pages: 4117-4121

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Characteristics of Patients with Prostate Cancer Who Have Initially been Treated by Hormone Therapy in Japan : J-CaP Surveillance2004

    • Author(s)
      Akaza H
    • Journal Title

      Japanese Journal of Clinical Oncology 34・6

      Pages: 329-336

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Comparison between the gene expression profiles of non-invasive and invasive human urinary bladder transitional cell carcinoma phenotypes using cDNA microarray : preliminary results

    • Author(s)
      Essam Elsamman
    • Journal Title

      Urologiconcology (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Prostate stem cell antigen predicts tumor recurrence in superficialtransitional cell carcnoma of the urinary bladder

    • Author(s)
      Essam Elsamman
    • Journal Title

      BJU INTERNATIONAL (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Comparison between the gene expression profiles of non-invasive and invasive human urinary bladder transitional cell carcinoma phenotypes using cDNA microarray : preliminary results

    • Author(s)
      Essam Elsamman
    • Journal Title

      Urologiconcology (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Prostate stem cell antigen predicts tumor recurrence in superficial transitional cell carcnoma of the urinary bladder

    • Author(s)
      Essam Elsamman
    • Journal Title

      BJU INTERNATIONAL (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Comparison between the gene expression profiles of non-invasive and invasive human urinary bladder transitional cellcarcinoma phenotypes using cDNA microarray : preliminary results

    • Author(s)
      Essam Elsamman
    • Journal Title

      Urologiconcology in press

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Prostate stem cell antigen predicts tumor recurrence in superficial transitional cell carcnoma of the urinary bladder

    • Author(s)
      Essam Elsamman
    • Journal Title

      BJU INTERNATIONAL in press

    • Related Report
      2005 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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