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Classification of mechanism of androgen independency in prostate cancer

Research Project

Project/Area Number 16591606
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionSapporo Medical University

Principal Investigator

MASUMORI Naoya  Sapporo Medical University, School of Medicine, Assistant Professor, 医学部, 講師 (20295356)

Co-Investigator(Kenkyū-buntansha) TSUKAMOTO Taiji  Sapporo Medical University, School of Medicine, Professor, 医学部, 教授 (50112454)
TAKAHASHI Atsushi  Sapporo Medical University, School of Medicine, Assistant Professor, 医学部, 講師 (20274946)
KATO Kazunori  Sapporo Medical University, School of Medicine, Associate Professor, 医学部, 助教授 (60233780)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2005: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2004: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordsprostate cancer / neuroendocrine cell / metastasis / invasion / gelsolin
Research Abstract

<Background> : Although neuroendocrine (NE) cells in prostate cancer have been speculated to accelerate the growth and progression of surrounding cancer cells, direct evidence is insufficient. We investigated the effect of an NE allograft (NE-10) and its cell line, NE-CS, which were established from the prostate of the LPB-Tag 12T-10 transgenic mouse, on the human prostate cancer cell line LNCaP.
<Method>s : The proliferation and pulmonary metastasis of LNCaP xenografts in athymic mice with and without NE-10 allografts were evaluated. Boyden chamber assay and microarray analysis was performed to investigate change in invasion/migration and mRNA of LNCaP cells under the influence of the NE cells, respectively.
Results : The NE-10 did not influence the proliferation of the LNCaP. The pulmonary metastasis of LNCaP with the NE-10 significantly increased compared to mice without it. The NE-CS cells accelerated the in vitro invasion/migration of adenocarcinoma cells. Increased expression of mRNA of gelsolin in LNCaP cells under the incubation with the supernatant of NE-CS cells was observed.
<Conclusion>s : The NE-10 allograft promotes pulmonary metastasis of subcutaneously inoculated LNCaP cells by facilitating cell invasion. Secretions from NE cells up-regulated expression of gelsolin, which is an actin-binding protein and resulted in acceleration of the migration of LNCaP cells.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (11 results)

All 2006 2005 2004 Other

All Journal Article (11 results)

  • [Journal Article] Murine androgen-independent neuroendocrine carcinoma promotes metastasis of human prostate cancer cell line LNCaP2006

    • Author(s)
      Uchida K, Masumori N, Takahashi A, Itoh N, Kato K, Tsukamoto T, et al.
    • Journal Title

      Prostate 66

      Pages: 536-545

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Characterization of prostatic neuroendocrine cell line established from neuroendocrine carcinoma of transgenic mouse allograft model.2005

    • Author(s)
      Uchida K, Masumori N, Takahashi A, Itoh N, Tsukamoto T
    • Journal Title

      The Prostate 62

      Pages: 49-53

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effectiveness of adjuvant intermittent endocrine therapy following neoadjuvant endocrine therapy and external beam radiation therapy in men with locally advanced prostate cancer.2005

    • Author(s)
      Yamanaka H, Itoh K, Naito S, Tsukamoto T, et al.
    • Journal Title

      The Prostate 63

      Pages: 56-64

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Murine androgen-independent neuroendocrine carcinoma promotes metastatis of human prostate cancer cell line LNCaP2005

    • Author(s)
      Uchida K, Masumori N, Takahashi A, Itoh N, Kato K, Tsukamoto T
    • Journal Title

      The Prostate 66

      Pages: 536-545

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Characterixzation of prostatic neuroendocrine cell likne established from neuro-endocrine carcinoma of transgenic mouse allograft model.2005

    • Author(s)
      Uchida K, Masumori N, Takahashi A, Itoh N, Tsukamoto T
    • Journal Title

      The Prostate 62

      Pages: 49-53

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] NE-10 neuroendocrine cancer promotes the LNCaP xenograft growth in castrated mice.2004

    • Author(s)
      Jin RJ, Wang Y, Masumori N, Ishii K, Tsukamoto T, et al.
    • Journal Title

      Cancer Research 64

      Pages: 5489-5495

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] An allograft model of androgen independent prostatic neuroendocrine carcinoma derived from a large probasin promoter-T antigen transgenic mouse line.2004

    • Author(s)
      Masumori N, Tsuchiya K, TuW, LEE c, Kasper S, Tsukamoto T, et al.
    • Journal Title

      Journal of Urology 171

      Pages: 439-442

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] An allograft model of androgen independent prostatic neuroendocrine carcinoma derived from a larfe probasin promoter-T antigen transgenic mouse line.2004

    • Author(s)
      Masumori N, Tsuchiya K, Tu W, Lee C, Kasper S, Tsukamoto T, et al.
    • Journal Title

      Journal of Urology 171

      Pages: 439-442

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] NE-10 neuroendocrine cancer promotes the LNCaP xenograft growth in castrated mice2004

    • Author(s)
      Jin RJ
    • Journal Title

      Cancer Research 64・15

      Pages: 5489-5495

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Characterization of prostatic neuroendocrine cell line established from neuroendocine carcinoma of transgenic mouse allograft model2004

    • Author(s)
      Uchida K
    • Journal Title

      Prostate 62・1

      Pages: 40-48

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Murine androgen-independent neuro-endocrine carcinoma promotes metastasis of human prostate cancer cell line LNCaP.

    • Author(s)
      Uchida K, Masumori N, Takahashi A, Itoh N, Kato K, Tsukamoto T
    • Journal Title

      The Prostate 55

      Pages: 536-545

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary

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Published: 2004-04-01   Modified: 2016-04-21  

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