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BASIC STUDY FOR EPIGENETIC EFFECT(S) CAUSED BY ASSISTED REPRODUCTIVE TREATMENT ON THE GERM-CELL/ZYGOTE

Research Project

Project/Area Number 16591635
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionJUNTENDO UNIVERSITY

Principal Investigator

ARAKI Yoshihiko  JUNTENDO UNIVERSITY, INSTITUTE FOR ENVIRONMENTAL & GENDER-SPECIFIC MEDICINE, ASSOCIATE PROFESSOR, 大学院・医学研究科, 助教授 (70250933)

Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2004: ¥2,500,000 (Direct Cost: ¥2,500,000)
KeywordsEPIGENETICS / DNA METYLATION / IN VITRO FERTILIZATION / FEMALE REPRODUCTIVE TRACT / GERMCELL / CpGアイランド
Research Abstract

THERE IS GROWING EVIDENCE THAT EPIGENETIC CHANGE OF GENOMIC DNA PLAYS AN IMPORTANT ROLE FOR TISSUE-SPECIFIC MOLECULAR EXPRESSION. SINCE THESE CHANGES ARE EASILY CONTROLLED BY SEVERAL ARTIFICIAL FACTORS INCLUDING ENVIRONMENTAL FACTOR< THE RELATIONSHIP BETWEEN THE EPIGENETIC CHANGE OF GENOME AND THE LIFESTYLE-RELATED ILLNESSES ARE FOCUSED ON CLINICALLY. THE PRIMARY OBJECTIVE OF THIS RESEARCH IS TO EXAMINE THE EPIGENETIC EFFECT OF ARTIFICIAL REPRODUCTIVE TECHNIQUE(S) ON GAMETE/ZYGOTE AND EARLY EMBRYONAL DEVELOPMENT. AS AN INITIAL STEP< WE TRIED TO ESTABLISH A SINGLE CELL PCR TECHNIQUE, BECAUSE AT MOST 20-30 EGGS ARE RECOVERED FROM AN ADULT MOUSE WITHOUT TREATMENT OF SUPEROVULATION. SUBSEQUENTLY, WE CHARACTERIZED TEX101 GENE, RECENTLY IDENTIFIED AS A GERM-CELL-SPECIFIC MARKER, FOR A CANDIDATE OF THE TARGET GENE SHOWING EPIGENETIC CHANGE AFTER ENVIRONMENTAL EXPOSURE. DELETION ANALYSIS OF THE CHIMERIC STRUCTURES INDICATED THAT SEQUENCES ESSENTIAL TO GENE EXPRESSION ARE PRESENT ON THE 5'-FLANKING REGION BETWEEN 3186 AND +14, WHERE THE CLUSTER OF FIVE CAAT BOXES IS LOCATED. THESE FINDINGS SHOULD FACILITATE AN UNDERSTANDING OF THE REGULATION OF TEX 101 EXPRESSION DURING GAMETOGENESIS, AND MAY BE USEFUL FOR FURTHER STUDIES OF THE GENE INCLUDING EPIGENETIC ANALYSIS.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (12 results)

All 2006 2005 2004

All Journal Article (12 results)

  • [Journal Article] Molecular characterisation of a germ cell-specific antigen, TEX101, from mouse testis.2006

    • Author(s)
      Jin H., et al.
    • Journal Title

      Zygote (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Epididymis-specific promoter-driven gene targeting : A transcription factor which regulates epididymis-specific gene expression.2006

    • Author(s)
      Suzuki K., et al.
    • Journal Title

      Molecular and Cellular Endocrinology (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Molecular characterisation of a germ cell-specific antigen, TEX101,from mouse testis.2006

    • Author(s)
      Jin H., et al.
    • Journal Title

      Zygote (in press.)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Epididymis-specific promoter-driven gene targeting : A transcription factor which regulated epididymis-specific gene expression.2006

    • Author(s)
      Suzuki K., et al.
    • Journal Title

      Mol.Cell.Endocrinol. (in press.)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Sexually dimorphic expression of the novel germ cell antigen TEX101 during mouse gonad development.2005

    • Author(s)
      Takayama T., et al.
    • Journal Title

      Biology of Reproduction 72

      Pages: 1315-1323

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Inhibition of cell spreading and stress fiber formation in CHO cells transfected with cDNA of a glycosylphosphatidylinositol-anchored glycoprotein, GPI-80.2005

    • Author(s)
      Anzai-Takeda Y., et al.
    • Journal Title

      Immunobiology 210

      Pages: 1-10

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] TEX101 is shed from the cell surface of sperm located in the caput epididymidis of the mouse.2005

    • Author(s)
      Takayama T., et al.
    • Journal Title

      Zygote 13

      Pages: 325-333

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Elevation of the soluble form GPI-80, a β2 integrin-associated glycosylphosphatidylinositol anchored protein, in the serum of patients with Weacner's vranulomatosis.2005

    • Author(s)
      Yoshitake H., et al.
    • Journal Title

      Allergology International 54

      Pages: 299-303

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Elevation of the soluble form CPI-80, a β2 integrin-associated glycosylphosphatidylinositol anchored protein, in the serum of patients with Wegener's granulomatosis.2005

    • Author(s)
      Yoshitake H., et al.
    • Journal Title

      Allergology International 54

      Pages: 299-303

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Sexually dimorphic expression of the novel germ cell antigen TEX101 during mouse gonad development2005

    • Author(s)
      Takayama et al.
    • Journal Title

      Biol Reprod (in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Molecular evolution of a multigene subfamily encoding epididymal lipocalins localized on the [A3] locus of mouse chromosome 2.2004

    • Author(s)
      Suzuki et al.
    • Journal Title

      Gene 339

      Pages: 49-59

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Characteristics of invasive cells found in between zona pellucida and oocyte during follicular atresia in mice2004

    • Author(s)
      Kamo et al.
    • Journal Title

      Zygote 12

      Pages: 269-276

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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