| Project/Area Number |
16591647
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Toyama university (2005)
Toyama Medical and Pharmaceutical University (2004) |
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Principal Investigator |
SASAKI Yasushi Toyama university, obstetrics and gynecology, assistant, 附属病院, 助手 (60324050)
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| Co-Investigator(Kenkyū-buntansha) |
SAITO Sigeru Toyama university, obstetrics and gynecology, professor, 大学院医学薬学研究部, 教授 (30175351)
MIYAZAKI Satomi Toyama university, obstetrics and gynecology, assistant, 大学院医学薬学研究部, 助手 (90361956)
SASAKI Masatoshi Toyama university, obstetrics and gynecology, assistant professor, 附属病院, 助教授 (90242502)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2004: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | CD4^+CD25^+ regulatory T cells / decidua / early pregnancy / spontaneous abortion |
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| Research Abstract |
A immunosuppression or tolerance is important to maintenance for pregnancy. Recent data showed that regulatory CD4^+ T cells express IL-2 receptor a (CD25) on their surface and suppress the immune responses of T cells through CTLA-4 molecule. We reported that regulatory T cells increased in earlypregnancy decidua, while Treg ratio in spontaneous abortion cases did not increase. These results suggest the increased regulatory T cells in the decidua play some roles for maintenance of pregnancy by regulating maternal cytotoxic T cells. In this study, we investigate whether the administration of anti mouse CD25 monoclonal antibody (PC61) induce abortion in early pregnant mouse. BALB/C male and C57BL/6 female mouse were crossbred and pregnant mouse was given intraperitoneal injection of anti-CD25 mAb(0.5mg/body x2) on the 4th and 7th day of gestation. Control mouse was given saline as a control. Then the mice were sacrificed on day 11th and examined the number of viable and aborted fetuses. 1.When anti-CD25 mAb was injected to pregnant BALB/C mice, CD4^+CD25^+ cells disappeared on day 11th of gestation. 2.There was no differences between the number of total fetuses of anti-CD25 mAb injected groups and that of control groups. On the other hand, abortion ratio of anti-CD25 mAb group was significantly higher than that of control group (37.6±39.6% vs. 0%, p=0.0441). In allogenic mice, inhibition of IL-2 receptor a induced abortion, however this mAb treatment did not affect the abortion rates in syngenic pregnancy. Regulatory T cells play an important role for protection of maternal allogeneic rejection.
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