| Project/Area Number |
16591656
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Osaka University |
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Principal Investigator |
ENOMOTO Takayuki Osaka University, Graduate School of Medicine, Lecture, 医学系研究科, 講師 (90283754)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHINO Kiyoshi Osaka University, Graduate School of Medicine, Assistant, 医学系研究科, 助手 (90362730)
FUJITA Masami Osaka University, Graduate School of Medicine, Assistant, 医学系研究科, 助手 (60303963)
中嶌 竜一 大阪大学, 医学系研究科, 助手 (70335347)
上田 豊 大阪大学, 医学系研究科, 助手 (10346215)
岡澤 美佳 大阪大学, 医学部附属病院, 医員(臨床研究)
三宅 貴仁 大阪大学, 医学部附属病院, 医員(臨床研究)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2004: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | TSC403 / DC-Lamp / cervical cancer / metastases / invasion / prognostic / DC-Lamp |
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| Research Abstract |
In order to clarify the role of the TSC403/DC-Lamp gene in uterine cervical cancer, especially concerning metastasis, following experiments were designed. Quantitative RT-PCR showed TSC403/DC-Lamp mRNA expression was not detectable in normal uterine cervical tissue but highly expressed in uterine cervical cancer. In addition, high expression level of TSC403/DC-Lamp was significantly correlated with the overall survival of patients with stage I/II cervical cancer. These indicate that TSC403/DC-Lamp expression may be a prognostic molecule marker for cervical cancer. We also transfected a TSC403/DC-LAMP expression vector into a human uterine cervical cancer cell line, TCS. In vitro invasion assay showed the migration of TSC403/DC-LAMP-overexpressing TCS cells was significantly higher than in control TCS cells. In vivo metastasis assay demonstrated distant metastasis was significantly higher in TSC403/DC-lamp-overexpressing TSC cells than in control TCS cells. These results indicate that expression of TSC403/DC-Lamp associates with invasion and metastasis in uterine cervix cancer. Furthermore, we identified a promoter domain of a TSC403 gene to elucidate molecule mechanism of overexpression of a TSC403 gene. To examine the localization of TSC403/DC-Lamp protein in the cell, we have established the GFP-tagged vector and transfected to cervical cancer cell. TSC403/DC-Lamp protein was shown to be expressed specifically on a cell membrane in uterine cervix cancer cell.
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