| Project/Area Number |
16591673
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Oita University |
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Principal Investigator |
NARAHARA Hisashi OITA UNIVERSITY, DEPT. OF OBSTET. & GYNECOL., PROFESSOR (60211447)
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| Co-Investigator(Kenkyū-buntansha) |
KAWANO Yasushi OITA UNIV., DEPT. OF OBSTET. & GYNECOL., ASSISTANT PROFESSOR (40274758)
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2006: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 2005: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 2004: ¥3,200,000 (Direct Cost: ¥3,200,000)
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| Keywords | platelet-activating factor / implantation / endometrium / decidua / angiogenesis |
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| Research Abstract |
Platelet-activating factor (PAF), a potent lipid mediator, has been implicated in a number of reproductive processes, including implantation. PAF has been shown to be produced by human embryo, which appears to be a requisite for viability.However the precise role of embryo-derived PAF in implantation has yet to be established. To investigate the role of PAF in endometrial or decidual angiogenesis, we evaluated the production of various angiogenic factors or anti-angiogenic factors by endometrial stromal cells (ESC) or decidual cells (DC). We measured the concentrations of three angiogenic factors in the culture media of ESC and DC following stimulation with an analogue, carbamyl-PAF (C-PAF). This substrate is not metabolized. These three factors were vascular endothelial growth factor (VEGF), basic fibroblast growth factor (b-FGF) and angiogenin. Enzyme-linked immunosorbent assay was used. In addition, we evaluated the expression of thrombospondin (TSP)-1 protein in ESC and DC after stimulation with C-PAF using western blot analysis. The non-stimulated ESC constitutively secreted VEGF, b-FGF, and angiogenin. The levels of these angiogenic factors in the culture media of the stimulated ESC and DC rose in accordance with an increase in the amount of C-PAF added. The C-PAF-induced stimulation in DC was two or three times more potent than that in ESC. These C-PAF-induced effects were abolished following pretreatment with PAF receptor antagonists, Y-24180 and CV2086. C-PAF had no effect on TSP-1 production in ESC and DC. It is suggested that the PAF secreted by the decidual tissue and by the developing embryo stimulates the production of these angiogenic factors at the feto-maternal interface. The up-regulation of these factors in the local environment may be important in the angiogenesis during early stages of gestation.
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