| Project/Area Number |
16591677
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | International University of Health and Welfare |
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Principal Investigator |
YANAGIDA Kaoru International University of Health and Welfare, School of Health Science, Professor, 保健学部(臨床医学研究センター), 教授 (10182370)
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| Co-Investigator(Kenkyū-buntansha) |
HAYASHI Syotaro Fukushima Medical University, School of medicen Department of Obstetrics and Gynecology, Assistant, 医学部産婦人科, 助手 (60372827)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2005: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2004: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | infertility / fertilization failure / hyperactivation / acrosome reaction / IVF / ICSI / unexplained infertility / oocyte activation |
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| Research Abstract |
With the clinical condition that the fertilization failure does not become clear if we do not perform IVF and ICSI, early diagnosis is expected. The fertilization failure of IVF occurred in 13.5% of treatment cycle, and the recurrence rate was 30-40%. The fertilization failure of ICSI occurred in 5.6% of treatment cycle, and the recurrence rate was 13%. We examined computer analysis of sperm motility, the measurement of acrosome enzyme activity, evaluation of nucleoprotein by acridine orange coloring as examination to detect the fertilization failure. Each parameter of CASA's values was not the good parameters detected the fertilization failure. However there was some correlation fertilization rate with CLV and BCF. Future examination seemed to be need. We could set cut off value of fertilization when we evaluated sperm-hyaluronidase activity in zymogenic assay (1.7mm). The value of zymogenic assay (ZGA) 1.7 was as follows, and pregnancy case was not present about AIH and IVF. Positive correlation was found in ZGA and fertilization rate, and ZGA may become a test for the detection of fertilization failure. A fertilization failure was found in the 50% in the immature nucleoprotein cases by acridine orange stain, and it seemed to be superior methods as a fecundation disorder test for the detection.
When the fertilization failure is concerned about in IVF, we adopt rescue ICSI. Rescue ICSI is ICSI to perform after insemination of IVF in six hours. When last ICSI was the fertilization failure, we perform ICSI in the half oh retrieved oocytes and perform egg activation combination ICSI in remaining half (split activation). When the fertilization failure is concerned about this ICSI, we perform a fertilization judgment six hours of ICSI later. And when there is not a fertilized egg, we carry out egg activation (rescue activation).
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