| Project/Area Number |
16591681
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | International University of Health and Welfare (2005-2007)
Dokkyo Medical University (2004) |
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Principal Investigator |
HAYASHI Masatoshi International University of Health and Welfare, Department of Obstetrics and Gynecology, Professor (90164962)
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| Co-Investigator(Kenkyū-buntansha) |
OHKURA Takeyoshi Dokkyo Medical University Koshigaya Hospital, Department of Obstetrics and Gynecology, Professor (80092394)
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| Project Period (FY) |
2004 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,450,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥150,000)
Fiscal Year 2007: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2006: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2005: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2004: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | preeclampsia / normal pregnancies / M-CSF / GM-CSF / IL-6 / TNF-a / placenta / amniotic fluid / macrophage colony-stimulating factor / liquid content / ovarian tumor / serous cystadenoma / mucinous cystadenoma / mature cystic teratoma / preeclampsia / amniotic fluid / tumor necrosis factor-α / interleukin-6 / granulocyte-macrophage colony-stimulating factor / serum / placenta / 血清 / total protein / ELISA / 免疫組織染色 |
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| Research Abstract |
Serum levels of tumor necrosis factor-α (TNF-α), Th1 cytokine, were higher in preeclampsia than in normal pregnancies. However, serum levels of interleukin-6 (IL-6), Th2 cytokine, and both TNF-α and IL-6 levels in the placenta did not differ between the two groups. These findings suggest that serum TNF-α is involved in the pathophysiology of preeclampsia but that in the placenta neither TNF-α nor IL-6 play an important role. Amniotic fluid IL-6 levels did not differ between the two groups, suggesting that IL-6 is not involved in the pathophysiology of this condition. Significantly lower birth weights of neonates were seen in preeclampsia compared with those in normal pregnancies. Thus, placental dysfunction might be occurred in preeclampsia. However, IL-6 did not play an important role in the placental dysfunction. Amniotic fluid macrophage colony-stimulating factor (M-CSF) concentrations were significantly higher in women who delivered small-for-gestational-age (SGA) infants than in women who delivered appropriate-for-gestational-age (AGA) infants. Excessive concentrations of M-CSF in amniotic fluid via amniotic membranes may be highly related to immunological abnormalities contributing to low birth weight of neonates. There was no significant increase in granulocyte-macrophage colony-stimulating factor (GM-CSF) levels in amniotic fluid during labor compared to that before the onset of labor. Thus, GM-CSF in amniotic fluid may not promote the onset of labor at term and/or term labor contributing to subsequent delivery may not induce the production and secretion of GM-CSF into amniotic cavity. Amniotic fluid M-CSF concentrations were significantly higher in term gravidas than in women at 17 weeks of gestation. This finding suggests that elevated levels of M-CSF in amniotic fluid have an important immunological function in the maintenance of pregnancy and fetal growth.
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