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Antitumor effect of cell-mediated immunity for head and neck squamous cell carcinoma

Research Project

Project/Area Number 16591707
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionKobe University

Principal Investigator

ISHIDA Haruhiko  Kobe University, Graduate School of Medicine, Associate Professor, 大学院医学系研究科, 助教授 (70223005)

Co-Investigator(Kenkyū-buntansha) NIBU Ken-ichi  Kobe University, Graduate School of Medicine, Professor, 大学院医学系研究科, 教授 (20251283)
OTSUKI Naoki  Kobe University Hospital, Lecturer, 医学部附属病院, 講師 (40343264)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2006: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2005: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2004: ¥2,700,000 (Direct Cost: ¥2,700,000)
KeywordsB7-1(CD80) / KLN205 cell line / DBA / 2 mouse / antitumor effect / vaccine effect / CD8 / MHC Class I / 細胞性免疫 / CD80(B7-1) / KLN-205 / 樹状細胞
Research Abstract

A murine squamous cell carcinoma cell line, KNL205, was infected with adenoviral vectors carrying either B7-1 (AdB7) or LacZ gene (AdCL). Infected cells were injected subcutaneously into the flank of DBA/2 mice. AdB7-infected cells grew significantly slower than AdCL-infected cells. Significant growth suppression of re-challenged non-infected parental cells was observed in the mice immunized with AdB7-infected cells but not in those immunized with AdCL-infected cells.
Immunohistochemical study was performed to analyze the production of CD80 (product of the B7-1 gene) and of MHC class I antigen, as well as the type of T lymphocytes in the tumor tissues. Positive staining for CD80 was detected in the majority of tumor cells in the AdB7-infected group, but only in occasional cells in the AdCL-infected or non-infected group. Staining for the MHC Class I antigen was observed in most of the tumor cells in all three groups. A large number of CD8-positive cells were seen in AdB7-infected tumors, while there were few in the other two groups. CD4-positive cells were rare in all three groups.
These results indicate that induced B7-1 gene expression provided AdB7-infected SCC cells with a function as APC (antigen presenting cell) such as dendritic cell. They then activated CD8-positive T lymphocytes by means of CD80 costimulation with MHC class I, resulting in the anti-tumor effect. Moreover, in the "vaccine experiment", an anti-tumor effect was attained in the mice immunized with AdB7-infected cells, suggesting that vaccination augments not only local but also systemic immune response. Further studies to achieve high-level and long-acting expression of B7-1 and to examine the synergistic effect of various cytokines may lead to the clinical application of B7-1 gene transfer as a new therapeutic alternative for head and neck cancers.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (43 results)

All 2007 2006 2005 2004 Other

All Journal Article (43 results)

  • [Journal Article] Adenovirus-mediated gene transfer of B7-1 induces immunological anti-tumor effects in a murine squamous cell carcinoma2007

    • Author(s)
      Hoshitani Y
    • Journal Title

      Arch Otolaryngol Head Neck Surg (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Efficacy and feasibility of cisplatin-based concurrent chemoradiotherapy for nasopharyngeal carcinoma2006

    • Author(s)
      Demizu Y
    • Journal Title

      Jpn J Clin Oncol 36・10

      Pages: 620-625

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Surgical strategy for lymph node metastases of oropharyngeal squamous cell carcinoma2006

    • Author(s)
      Shimizu K
    • Journal Title

      Acta Otolaryngol 126

      Pages: 872-877

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Simple reconstruction with titanium mesh and radial forearm flap after globe-sparing total maxillectomy2006

    • Author(s)
      Hashikawa K
    • Journal Title

      Plast Reconstr Surg 117

      Pages: 963-967

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Quality of life after neck dissection2006

    • Author(s)
      Inoue H
    • Journal Title

      Arch Otolaryngol Head and Neck Surg 132

      Pages: 662-666

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A paranasal tumor associated with tumor-induced osteomalacia2006

    • Author(s)
      Inokuchi G
    • Journal Title

      Laryngoscope 116

      Pages: 1930-1933

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] オスラー病の鼻出血にどう対処するか? レーザー手術の立場から2006

    • Author(s)
      石田春彦
    • Journal Title

      JOHNS 22

      Pages: 1409-1412

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 鼻副鼻腔悪性腫瘍の拡大手術2006

    • Author(s)
      石田春彦
    • Journal Title

      日本鼻科学会誌 45

      Pages: 102-103

    • NAID

      10018115267

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] せきーその診断と治療 喉頭アレルギー2006

    • Author(s)
      石田春彦
    • Journal Title

      ENTONI 59

      Pages: 39-45

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 花粉症と咳そう2006

    • Author(s)
      石田春彦
    • Journal Title

      Modern Physician 26

      Pages: 1731-1733

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Efficacy and feasibility of cisplatin-based concurrent chemoradiotherapy for nasopharyngeal carcinoma2006

    • Author(s)
      Demizu Y
    • Journal Title

      pn J Clin Oncol 36-10

      Pages: 620-625

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 鼻副鼻腔悪性腫瘍の拡大手術2006

    • Author(s)
      石田春彦
    • Journal Title

      日本鼻科学会会報 45・1

      Pages: 102-104

    • NAID

      10018115267

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 鼻腔放線菌による鼻石の一例2006

    • Author(s)
      蔵川涼世
    • Journal Title

      日本鼻科学会会報 45・1

      Pages: 8-11

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 喉頭アレルギー2006

    • Author(s)
      石田春彦
    • Journal Title

      ENTONI 59

      Pages: 39-45

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Immunohistochemical study on distribution of mast cell phenotypes in human laryngeal mucosa : Evidence for laryngeal type 1 allergy2005

    • Author(s)
      Ishida H
    • Journal Title

      Annals of Otology, Rhinology & Laryngology 114・2

      Pages: 139-143

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Adenovirus-mediated gene transfer in olfactory epithelium and olfactory bulb : A long-term study2005

    • Author(s)
      Doi K
    • Journal Title

      Annals Otology, Rhinology & Laryngology 114

      Pages: 629-633

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 側頭骨軟骨芽細包腫の一例2005

    • Author(s)
      米澤宏一郎
    • Journal Title

      Otology Japan 15

      Pages: 161-166

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 耳下腺に発生した悪性リンパ腫の検討2005

    • Author(s)
      李佳奈
    • Journal Title

      耳鼻咽喉科・頭頸部外科 77

      Pages: 741-746

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] 眼窩前頭部コレステリン肉芽腫の1例2005

    • Author(s)
      金城東和
    • Journal Title

      日本鼻科学会会誌 44

      Pages: 136-140

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 花粉症における咳そうと咽喉頭異常感2005

    • Author(s)
      石田春彦
    • Journal Title

      アレルギー科 19

      Pages: 212-217

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Immunohistochemical study on distribution of mast cell phenotypes in human laryngeal mucosa : Evidence for laryngeal type 1 allergy2005

    • Author(s)
      Ishida H
    • Journal Title

      Annals of Otology, Rhinology & Laryngology 114-2

      Pages: 139-143

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Adenovirus-mediated gene transfer in olfactory epithelium and olfactory bulb : A long-term study2005

    • Author(s)
      K.Doi
    • Journal Title

      Annals of Otology, Rhinology, & Laryngology 114

      Pages: 629-633

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 側頭骨軟骨芽細胞腫の一例2005

    • Author(s)
      米澤宏一郎
    • Journal Title

      Otology Japan 15

      Pages: 161-165

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 眼窩前頭部コレステリン肉芽腫に1例2005

    • Author(s)
      金城東和
    • Journal Title

      日本鼻科学会誌 44

      Pages: 136-140

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 花粉症における咳嗽と咽喉頭異常感2005

    • Author(s)
      石田春彦
    • Journal Title

      アレルギー科 19

      Pages: 212-217

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Immunohistochemical study on distribution of mast cell phenotypes in human laryngeal mucosa : Evidence for laryngeal type I allergy2005

    • Author(s)
      Ishida H
    • Journal Title

      Annals of Otology, Rhinology & Laryngology 114・2

      Pages: 139-143

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 顎下部に発生した脂肪肉腫の1例2005

    • Author(s)
      原 聡
    • Journal Title

      耳鼻咽喉科・頭頸部外科 77・2

      Pages: 133-137

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Quantitative analysis of NeuroD, Gap43, and receptor tyrosine kinase in developing mouse olfactory neuroepithelium2004

    • Author(s)
      Yasui R
    • Journal Title

      Acta Oto-Laryngologica 553

      Pages: 90-94

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Notch expression in developing olfactory neuroepithelium2004

    • Author(s)
      Doi K
    • Journal Title

      Neuroreport 15・6

      Pages: 945-947

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A rare spontaneous temporal meningoencephalocele with dehiscence into the pterygoid fossa2004

    • Author(s)
      Nishikawa T
    • Journal Title

      Auri Nasus Larynx 31

      Pages: 429-431

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 鼻茸組織おけるVascular Endothelial Growth Factorの発現2004

    • Author(s)
      石田春彦
    • Journal Title

      日本鼻科学会会誌 143・2

      Pages: 194-199

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 当科で経験した副鼻腔真菌症の検討2004

    • Author(s)
      武木田誠一
    • Journal Title

      日本鼻科学会会誌 143・4

      Pages: 375-379

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] 手術範囲と術式-鼻腔悪性腫瘍2004

    • Author(s)
      石田春彦
    • Journal Title

      JOHNS 20・9

      Pages: 1310-1314

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] 鼻茸と増殖因子2004

    • Author(s)
      石田春彦
    • Journal Title

      JOHNS 20・12

      Pages: 1776-1780

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] 耳鼻咽喉科・頭頸部外科の癌化学療法レジメンー上顎癌2004

    • Author(s)
      石田春彦
    • Journal Title

      耳鼻咽喉科・頭頸部外科 76・13

      Pages: 973-977

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Notch expression in developing olfactory neuroepithelium2004

    • Author(s)
      Doi K
    • Journal Title

      Neuroreport 15-6

      Pages: 945-947

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Quantative analysis of NeuroD, GAP43 and receptor tyrosine kinase in developing mouse olfactory neuroepithelium2004

    • Author(s)
      Yasui R
    • Journal Title

      Acta Oto-Laryngologica 553

      Pages: 90-94

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Notch expression in developing olfactory neroepithelium2004

    • Author(s)
      Doi K
    • Journal Title

      Neuroreport 15・6

      Pages: 945-947

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A rare spontaneous temporal meningoencephalocele with dehiscence into the pterygoid fossa2004

    • Author(s)
      Nishikawa T
    • Journal Title

      Auris Nasus Larynx 31

      Pages: 429-431

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 鼻茸組織におけるVascular Endothelial Growth Factorの発現2004

    • Author(s)
      石田春彦
    • Journal Title

      日本鼻科学会会誌 143・2

      Pages: 194-199

    • NAID

      10013353229

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 耳鼻咽喉科・頭頸部外科の癌化学療法レジメン-上顎癌2004

    • Author(s)
      石田春彦
    • Journal Title

      耳鼻咽喉科・頭頸部外科 76・13

      Pages: 973-977

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Distribution and its impact of lymph node metastases in oropharyngeal cancer

    • Author(s)
      K.Shimizu
    • Journal Title

      Acta Otolaryngologica (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Simple reconstruction with titanium mesh and radial forearm flap after globe-sparing total maxillectomy : A 5-year follow-up study

    • Author(s)
      K.Hashikawa
    • Journal Title

      Plastic and Reconstructive Surgery (in press)

    • Related Report
      2005 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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