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The role of innate immunity and Toll-like receptors in the pathogenesis of middle ear inflammation

Research Project

Project/Area Number 16591709
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionShimane University

Principal Investigator

MURATA Akemichi (2005-2006)  shimane university, otorhinolaryngology, assistant, 医学部, 助手 (90346388)

佐藤 達明 (2004)  島根大学, 医学部, 講師 (30288518)

Co-Investigator(Kenkyū-buntansha) KAWAUCHI Hideyuki  shimane university, otorhinolaryngology, professor, 医学部, 教授 (50161279)
SHIMIZU Yasuhiko  shimane university, otorhinolaryngology, assistant, 医学部, 助手 (60418788)
石光 亮太郎  島根大学, 医学部, 講師 (90301291)
村田 明道  島根大学, 医学部, 助手 (90346388)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2006: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2005: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2004: ¥2,700,000 (Direct Cost: ¥2,700,000)
Keywordsepithelial cells / chronic rhinosinusitis / otitis media / mast cells / Toll-like receptor / RT-PCR / OK432 / IL-12 / アレルギー性鼻炎 / 早期介入療法 / TLR / IL-8
Research Abstract

We attempted to investigate the distribution of Toll-like receptor (TLR4 and TLR2) in upper respiratory epithelial cells such as human cell lines, by employing flowcytometry and northern blot analysis. And in in vitro study, the exact role of TLR2 and TLR4 in IL-8 and IL-15 production from upper respiratory epithelial cells was examined when these cells were stimulated with bacterial degradation product such as lipoprotein or lipopolysaccharide.
Results: 1) Respiratory epithelial cells constitutively expressed mRNA for TLR2, 3, 6, but not for TLR4 and TLR9. 2) Respiratory epithelial cells also expressed CD14 and MyD88. 3) Lipoprotein induced IL-15 production in respiratory epithelial cells, which strictly depend on TLR2. 4) Lipoprotein-mediated IL-8 and IL-15 production in respiratory epithelial cells was abolished by NF-kB inhibition. 5) Antihistamine downregulated in vitro lipoprotein-mediated IL-8 production from respiratory epithelial cells and in vivo inflammation in mouse acute rhinitis model as well. 6) OK432 administration inhibits murine allergic rhinitis at the induction phase, through the macrophage activation with TLR2 signaling pathway. 7) LPS exacerbates murine allergic rhinitis at the eliciting phase.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (21 results)

All 2006 2005 2004 2003 Other

All Journal Article (21 results)

  • [Journal Article] IL-15 prevents allergic rhinitis through reactivation of antigen-specific CD8+cells.2006

    • Author(s)
      Noriaki Aoi, Tokuko Masuda, Daisuke Murakami, Toshiki Yajima, Hiroyuki Mizubuchi, Hisakata Yamada, Hideyuki Kawauchi, Yasunobu Yoshikai
    • Journal Title

      Journal of Allergy and Clinical Immunology 117(6)

      Pages: 1359-1366

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 上気道アレルギー性炎症の制御に向けた研究の動向2006

    • Author(s)
      川内秀之
    • Journal Title

      アレルギー科 21(6)

      Pages: 597-605

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] IL-15 prevents allergic rhinitis through reactivation of antigen-specific CD8^+ cells.2006

    • Author(s)
      Noriaki Aoi, Tokuko Masuda, Daisuke Murakami, Toshiki Yajima, Hiroyuki Mizubuchi, Hisakata Yamada, Hideyuki Kawauchi, Yasunobu Yoshikai
    • Journal Title

      Journal of Allergy and Clinical Immunology 117(6)

      Pages: 1359-1366

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] IL-15 prevents allergic rhinitis through reactivation of antigen-specific CD8+ cells.2006

    • Author(s)
      Noriaki Aoi
    • Journal Title

      The Journal of allergy and clinical immunology 117・6

      Pages: 1359-1366

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A rice-based edible vaccine expressing multiple T cell epitopes induces oral tolerance for inhibition of Th2-mediated IgE responses.2005

    • Author(s)
      Hidenori Takagi, Takachika Hiroi, Lijun Yang, Yoshifumi Tada, Yoshikazu Yuki, Kaoru Takamura, Ryotaro Ishimitsu, Hideyuki Kawauchi, Hiroshi Kiyono, Fumio Takaiwa
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America. 102(48)

      Pages: 17525-17530

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A rice-based edible vaccine expressing multiple T cell epitopes induces oral tolerance for inhibition of Th2-mediated IgE responses2005

    • Author(s)
      Hidenori Takagi, Takachika Hiroi, Lijun Yang, Yoshifumi Tada, Yoshikazu Yuki, Kaoru Takamura Ryotaro Ishimitsu, Hideyuki Kawauchi, Hiroshi Kiyono, Fumio Takaiwa
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America 102(48)

      Pages: 17525-17530

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A rice-based edible vaccine expressing multiple T cell epitopes induces oral tolerance for inhibition of Th2-mediated IgE responses2005

    • Author(s)
      Hidenori Takagi
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America 102・48

      Pages: 17525-17530

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 鼻アレルギー性炎症を抑制する薬剤2005

    • Author(s)
      石光亮太郎
    • Journal Title

      ENTONI 57

      Pages: 24-32

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Mucosal immunity of nasopharynx : an experimental study in TCR-transgenic (OVA23-3)mice2005

    • Author(s)
      Takaya Yamada
    • Journal Title

      Rhinology 43

      Pages: 190-198

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 副鼻腔炎の保存的治療2005

    • Author(s)
      川内秀之
    • Journal Title

      耳鼻咽喉科・頭頸部外科 77・11

      Pages: 811-815

    • Related Report
      2005 Annual Research Report
  • [Journal Article] サイトカイン制御〜新治療を含めて2005

    • Author(s)
      石光亮太郎
    • Journal Title

      アレルギー免疫別冊 12・2

      Pages: 46-52

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Interleukin(IL)-15のマウスアレルギー性鼻炎モデル実効相に及ぼす影響についての検討.2005

    • Author(s)
      石光亮太郎
    • Journal Title

      日本鼻科学会会誌 44・1

      Pages: 43-44

    • NAID

      10015546802

    • Related Report
      2005 Annual Research Report
  • [Journal Article] マクロファージによるOK-432の認識機構についての検討2004

    • Author(s)
      村田明道, 石光亮太郎, 太神尚士, 森倉一朗, 高村薫, 梅原毅, 佐藤達明, 矢島俊樹, 吉開泰信, 川内秀之
    • Journal Title

      耳鼻咽喉科免疫アレルギー 22(4)

      Pages: 15-18

    • NAID

      10015423052

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 経鼻免疫による中耳・耳管の局所免疫応答の誘導 TCR-Tgマウスでの検討(第2報)2004

    • Author(s)
      川内秀之
    • Journal Title

      Otology Japan 14・4

      Pages: 616-616

    • NAID

      10020400740

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 急性中耳炎の内耳波及の10症例の検討2003

    • Author(s)
      木村光宏
    • Journal Title

      Otology Japan 13・5

      Pages: 603-608

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Oxatomide Inhibits Interleukin-8 Release from respiratory Epithelial Cells.

    • Author(s)
      Tsuyoshi Umehara, Ryotaro Ishimitsu, Akemichi Murata, Hisashi Ohga, Ichiro Morikura, Kaoru Takamura, Hideyuki Kawauchi
    • Journal Title

      International Immunopharmacology, submiited for publication (revised)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Japanese traditional medicine, Senn-kinn-naidaku-sann upregulates Toll-like receptor 4 and reduces murine allergic rhinitis

    • Author(s)
      Ichiro Morikura, Ryotaro Ishimitsu, Akemichi Murata, Hisashi Ohga, Kaoru Goda, Noriaki Aoi, Yasunobu Yoshikai, Hideyuki Kawauchi
    • Journal Title

      Rhinology (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Oxatomide Inhibits Interleukin-8 Release from Respiratory Epithelial Cells.

    • Author(s)
      Tsuyoshi Umehara, Ryotaro Ishimitsu, Akemichi Murata, Hisashi Ohga, Ichiro Morikura, Kaoru Takamura, Hideyuki Kawauchi
    • Journal Title

      International Immunopharmacology (submiited for publication, revised)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Japanese traditional medicine, Senn-kinn-naidaku-sann upregulates Toll-like receptor 4 and reduces murine allergic rhinitis

    • Author(s)
      Ichiro Morikura, Ryotaro Ishimitseu, Akemichi Murata, Hisashi Ohga, Kaoru Goda, Noriaki Aoi, Yasunobu Yoshikai, Hideyuki Kawauchi
    • Journal Title

      Rhinology (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The influence of tonsillar diseases and tonsillectomy on immune system

    • Author(s)
      Hideyuki Kawauchi
    • Journal Title

      International Journal of Pediatric Otorhinolaryngology (in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] アレルギー性鼻炎の制御に向けた治療戦略の確立

    • Author(s)
      川内秀之
    • Journal Title

      臨床免疫・アレルギー (印刷中)

    • Related Report
      2006 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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