| Project/Area Number |
16591711
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Kagawa University |
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Principal Investigator |
MORI Nozomu Kagawa University, Faculty of Medicine, Professor, 医学部, 教授 (90124883)
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| Co-Investigator(Kenkyū-buntansha) |
MIYASHITA Takenori Kagawa University, Faculty of Medicine, Assistant, 医学部, 助手 (60363214)
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2005: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | endolymphatic sac / ion transport / patch clamp technique / RT-PCR / in situ hydridization / regulatory factors of ion transport / Na-K-2C1コトランスポーター / P_<2Y> レセプター / Na-K-2Clコトランスポーター / P_<2Y>レセプター / 内耳 / パッチクランプ / イオンチャネル / メニエール病 / ナトリウム輸送 |
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| Research Abstract |
(Microfluorometrical study on ion channel in the ES)
The dynamic properties of sodium transport in the endolymphatic sac (ES) were investigated using SFBI/AM fluorescence measurement. The intracellular Na^+ concentration was measured in freshly dissected ES sheet from the intermediate portion of the ES of the guinea pig. The results showed the strong relationship between Na^+, K^+ATPase activity and mitochondrial staining, suggesting that mitochondria-rich ES epithelial cells (Type 1 cells, ca. 30% of ES cells) endowed with high Na+ permeability and Na^+, K^+-ATPase activity potentially contribute to sodium transport of the ES.
(Molecular biological study of ion channels and ion transporters)
The presence of Na-K-2Clcotransporter(NKCC) and P_<2Y> receptor and their subtypes in the ES of the rat was examined by molecular biological techniques. RT-PCR showed the presence of two subtypes of NKCC (NKCC1 and NKCC2) and four subtypes of P<2Y> receptor (P_2Y_1,P_2Y_2,P_2Y_4,P_2Y_6). The presence of NKCC2 (peculiar to the kidney) in the ES strongly suggests the absorptive function of the ES. RT-PCR confirmed the presence of P2Y receptors suggested by our previous electrophysiological study. We are now examining the localization of NKCC and P_<2Y> receptor in the ES.
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