| Project/Area Number |
16591717
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
|
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| Research Institution | University of Miyazaki |
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Principal Investigator |
MATSUDA Keiji University of Miyazaki, Faculty of Medicine, Assistant, 医学部, 助手 (40253835)
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| Co-Investigator(Kenkyū-buntansha) |
KAWANO Hirokazu University of Miyazaki, Faculty of Medicine, Associate Prof., 医学部, 講師 (20204745)
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| Project Period (FY) |
2004 – 2005
|
| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2005: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2004: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | hair cell / intact cochlea / potassium current / electrophysiology / patch-clump / potassium currents / TEA-sensitive potassium currents / 哺乳類蝸牛 |
|---|
| Research Abstract |
The hemi-cochlear technique has greatly facilitated the investigation of the proportional contributions of two different mechanisms in cochlear amplification, somatic motility by outer hair cells (OHCs) and hair bundle motor derived from stereocilia. We tried to take whole cell recordings from inner hair cells (IHCs) in hemi-cochlea. However, it was too tough to get giga-ohm seal formation from IHCs. Instead of hemi-cochlea, we measured potassium currents from isolated guinea pig IHCs. We showed that TEA-dependent potassium current from IHCs possess the kinetics of fast inactivation. Some enzymes using for IHCs dissociation affect these inactivation kinetics. In addition, we reviewed another mechanism of cochlear amplification, or somatic motility of OHC. We also mentioned possible clinical manifestation of prestin-caused hearing loss.
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