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Study of effects of antiglaucoma eye drops on glaucomatous optic neuropathy and ocular serface.

Research Project

Project/Area Number 16591738
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionHirosaki University

Principal Investigator

OHGURO Ikuyo  Hirosaki University, School of Medicine, lecturer, 医学部, 講師 (90305235)

Co-Investigator(Kenkyū-buntansha) OHGURO Hiroshi  Hirosaki University, School of Medicine, associate professor, 医学部, 助教授 (30203748)
NAKAZAWA Mitsuru  Hirosaki University, School of Medicine, professor, 医学部, 教授 (80180272)
MAMIYA Kazuhisa  Hirosaki University, University Hospital, Instructor, 医学部附属病院, 助手 (60344610)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2004: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywordsantiglaucoma eye drops / neuroprotection / ocular surface / NMDA / α-adrenergic receptor / extracellular matrix / MMP / TIMP / N-methyl-D-asartate / 網膜神経節細胞 / glial fibrillary acidic protein / 眼圧下降作用 / 濾過手術 / matrix metalloprotease / tissue inhibitor of metalloprotease
Research Abstract

Purpose : To study the effects of antiglaucoma eye drops on N-methyl-D-aspartate (NMDA)-induced retinal damage and ocular surface
Methods : Several antiglaucoma eye drops, β-blockers, α/β-blockers, an α_1-blocker, and α_2-agonist, and a prostaglandin derivative, were topically administrated to NMDA-treated rat eyes or non-treated rat eye, and the retinal thickness, the number of retrograde-labeled retinal ganglion cells (RGCs), the results of a cDNA microarray analysis, and the levels of matrix metalloprotease (MMP) and tissue inhibitor of matrix protease (TIMP) which regulate the metabolism of the extracellular matrix (ECM) were studied.
Results : Intravitreal administration of NMDA caused a significant decrease in the thickness of the retinal layers and induced upregulation of glial fibrillary protein (GFAP). Topical administration of β-blockers and a prostaglandin derivative showed almost no significant effects on retinal thickness, the number of RGCs, or expression of GFAP. In contrast, the α/β-blockers, the α_1-blocker, and the α_2-agonist showed preservation effects on retinal thickness and the the number of RGCs, and marked suppression of NMDA-induced upregulation of GFAP. Among 1101 genes related to cellular regulatory mechanisms, the expression of two genes, both for insulin-like growth factors, IGF-1 and ErbB3,was altered upon administration of the α/β-blockers, the α_1-blocker, and the α_2-agonist. In addition, topical administration of the α/β-blockers, the α_1-blocker, and the prostaglandin derivative induced upregulation of MMP and downregulation of TIMP of conjunctiva.
Conclusion : Our present study suggests that modulations of the α-adrenergic receptor, α_1-blocking, and α_2-stimulation, by antiglaucoma eye drops may cause beneficial effects on NMDA-induced retinal damage in the rat. Our results also suggests that the α/β-blockers, the α_1-blocker, and the prostaglandin derivative induce degradation of ECM, affcting filter surgery.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (18 results)

All 2005 2004

All Journal Article (17 results) Book (1 results)

  • [Journal Article] Optic disc characteristics assessed by evaluation of clinical optic disc photographs in glaucoma patients.2005

    • Author(s)
      Ohguro I, Ohguro H, et al.
    • Journal Title

      Hirosaki Med. J. 57

      Pages: 27-34

    • NAID

      110006198530

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Study of effects of antiglaucoma eye drops on N-Methyl-D-Aspartate-induced retinal damage.2005

    • Author(s)
      Metoki T, Ohguro I, et al.
    • Journal Title

      Jpn J Ophthalmol 49

      Pages: 453-461

    • NAID

      10019218837

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] 緑内障性視神経障害における眼圧非依存因子の検討-血小板凝集能との係わり-2005

    • Author(s)
      大黒幾代, 大黒 浩, 他
    • Journal Title

      あたらしい眼科 22

      Pages: 669-672

    • NAID

      10017292155

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] 抗緑内障点眼薬のFDTによる神経保護効果の検討2005

    • Author(s)
      竹内侯雄, 大黒幾代, 他
    • Journal Title

      眼科紀要 56

      Pages: 134-137

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Optic disc characteristics assessed by evaluation of clinical optic disc photographs in glaucoma patients.2005

    • Author(s)
      Ohguro I, Ohguro H, et al.
    • Journal Title

      Hirosaki Med J 57

      Pages: 27-34

    • NAID

      110006198530

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Optic disc characteristics assessed by evaluation of clinical optic disc photographs in glaucoma patients.2005

    • Author(s)
      Ohguro I, Ohguro H, et al.
    • Journal Title

      Hirosaki Med.J. 57

      Pages: 27-34

    • NAID

      110006198530

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 正常眼圧にもかかわらず、緑内障様視野異常や角膜径増大を呈した発達緑内障疑いの2例2005

    • Author(s)
      大黒幾代, 大黒 浩, 他
    • Journal Title

      眼科 47

      Pages: 889-894

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 弘前大学眼科における改良非穿孔トラベクレクトミーの手術成績2005

    • Author(s)
      山本陽子, 大黒幾代, 他
    • Journal Title

      あたらしい眼科 22

      Pages: 813-816

    • NAID

      10017292284

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Effects of matrix metalloproteinase-3 gene transfer by electroporation in glaucoma filter surgery.2004

    • Author(s)
      Mamiya K, Ohguro I, et al.
    • Journal Title

      Exp Eye Res 79

      Pages: 405-410

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 点眼薬による眼表面の反応 抗緑内障点眼薬の眼表面への影響と神経保護の検討2004

    • Author(s)
      大黒 浩, 大黒幾代, 他
    • Journal Title

      眼薬理 18

      Pages: 69-73

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effects of matrix metalloprotease-3 gene transfer by electroporation in glaucoma surgery.2004

    • Author(s)
      Mamiya K, Ohguro I, et al.
    • Journal Title

      Exp Eye Res 79

      Pages: 405-410

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 緑内障セミナー 48.緑内障と自己免疫疾患2004

    • Author(s)
      大黒幾代, ほか
    • Journal Title

      あたらしい眼科 21

      Pages: 777-778

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Effects of matrix metalloproteinase-3 gene transfer by electroporation in glaucoma filter surgery.2004

    • Author(s)
      Mamiya K, Ohguro I et al.
    • Journal Title

      Exp Eye Res 79

      Pages: 405-410

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 点眼薬による眼表面の反応 抗緑内障点眼薬の眼表面への影響と神経保護の検討2004

    • Author(s)
      大黒 浩, 大黒幾代, ほか
    • Journal Title

      眼薬理 18

      Pages: 69-73

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 正常眼圧緑内障患者の動脈硬化指数2004

    • Author(s)
      大黒幾代, ほか
    • Journal Title

      あたらしい眼科 21

      Pages: 397-400

    • NAID

      10013601040

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 緑内障セミナー 46.グルタミン酸網膜障害2004

    • Author(s)
      大黒幾代, ほか
    • Journal Title

      あたらしい眼科 21

      Pages: 491-492

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 緑内障濾過手術後に重篤な脈絡膜剥離を来たした続発緑内障の2症例2004

    • Author(s)
      大黒幾代, ほか
    • Journal Title

      眼紀 55

      Pages: 575-578

    • Related Report
      2004 Annual Research Report
  • [Book] Frontiers in Glaucoma2005

    • Author(s)
      大黒 浩, 大黒幾代, 他
    • Total Pages
      7
    • Publisher
      メディカルレビュー社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary

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Published: 2004-04-01   Modified: 2016-04-21  

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