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Development of medium size animal models with photoreceptor degeneraion and assessment of visual function from these animals for retinal prosthesis studies

Research Project

Project/Area Number 16591747
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionNagoya University

Principal Investigator

KONDO Mineo  Nagoya University, University Hospital, Assistant Professor, 医学部附属病院, 講師 (80303642)

Co-Investigator(Kenkyū-buntansha) TERASAKI Hiroko  Nagoya University, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (40207478)
NAKAMURA Makoto  Nagoya University, Graduate School of Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (60283438)
三宅 養三  名古屋大学, 大学院・医学系研究科, 教授 (30166136)
近藤 永子  名古屋大学, 医学部附属病院, 助手 (30335038)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2004: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordsretinal prosthesis / transgenic / medium size / rabbit / retintis pigmentosa / retina / animal model / electroretinogram / モデル動物 / ロドプシン
Research Abstract

Development of an artificial eye or retinal prosthesis requires an animal on which the retinal prosthetic device can be tested. Preferably, the size of the eye of these animals should be close to the size of the human eye. For an evaluation of the safety of a prosthetic eye, the transplantation can be made into animals with healthy eyes. But when considering the possibility of restoring visual function to patients who have lost vision to degenerative diseases of the retina, it would be preferable to transplant the prosthetic device into animals that have also lost their vision in order to verify any possible improvements in the visual function. This would require an animal model with damage of their photoreceptor cells, but with the ganglion cells still remaining functionally intact. In this study, we tried to create a medium-sized animal model, initially rabbits, in which the blindness is induced by degeneration of the photoreceptor cells. To accomplish this goal, the following three projects were performed. (1)Injection of special drugs into the vitreous body to cause photoreceptor degeneration. (2)Systemic application of special drugs to cause photoreceptor degeneration. (3)Induction of photoreceptor degeneration through genetic manipulation. Especially, we focused on the genetic manipulation to induce the photoreceptor degeneration. In this method, rabbits were produced with a mutated rhodopsin gene, which is one of the genes responsible for the retinitis pigmentosa in humans. The rabbit genome clone which includes the rhodopsin gene is isolated, and a vector including rhodopsin gene mutations (Pro347Leu mutation) were made. Then, this vector was injected into fertilized eggs. These animal models are thought to be useful for animal experiments for retinal prosthesis.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (23 results)

All 2006 2005 2004

All Journal Article (22 results) Book (1 results)

  • [Journal Article] Effect of axial length on laser spot size during photodynamic therapy : An experimental study in monkeys.2006

    • Author(s)
      Kondo M
    • Journal Title

      American Journal of Ophthalmology 141

      Pages: 214-215

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Contribution of retinal neurons to d-wave of primate photopic electroretinograms.2006

    • Author(s)
      Ueno S
    • Journal Title

      Vision Research 46

      Pages: 658-664

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Effect of axial length on laser spot size during photodynamic therapy: An experimental study in monkeys.2006

    • Author(s)
      Kondo, M.
    • Journal Title

      American Journal of Ophthalmology. 141

      Pages: 214-215

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Contribution of retinal neurons to d-wave of primate photopic electroretinograms.2006

    • Author(s)
      Ueno, S.
    • Journal Title

      Vision Research. 46

      Pages: 658-664

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Cone and rod dysfunction in fundus albipunctatus with RDH5 mutation : Electrophysilogical study.2005

    • Author(s)
      Niwa Y
    • Journal Title

      Investigative Ophthalmology & Visual Science 46

      Pages: 1480-1485

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Physilogical function of S-cone system is not enhanced in rd7 mice.2005

    • Author(s)
      Ueno S
    • Journal Title

      Experimental Eye Research 81

      Pages: 751-758

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Cone and rod dysfunction in fundus albipunctatus with RDH5 mutation: Electrophysiological study.2005

    • Author(s)
      Niwa, W.
    • Journal Title

      Investigative Opthalmology & Visual Science. 46

      Pages: 1480-1485

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Physiological function of S-cone system is not enhanced in rd7 mice.2005

    • Author(s)
      Ueno, S.
    • Journal Title

      Experimental Eye Research. 81

      Pages: 751-758

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Primate retinal signaling pathways : suppressing ON-pathway activity in monkey with glutamate analogues mimics human CSNB1-NYX genetic night blindness.2005

    • Author(s)
      Khan NW
    • Journal Title

      Journal of Neurophysiology 93

      Pages: 481-492

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Cone and rod dysfunction in fundus albipunctatus with RDH5 mutation : Electrophysiological study.2005

    • Author(s)
      Niwa Y
    • Journal Title

      Investigative Ophthalmology & Visual Science 46

      Pages: 1480-1485

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Acquired unilateral night blindness with negative ERG : Nine-year follow-up.2005

    • Author(s)
      Kondo M
    • Journal Title

      Retina 25

      Pages: 519-520

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Physiological function of S-cone system is not enhanced in rd7 mice.2005

    • Author(s)
      Ueno S
    • Journal Title

      Experimental Eye Research 81

      Pages: 751-758

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Cone and rod dysfunction in fundus albipunctatus with RDH5 mutation : Electrophysiological study.2005

    • Author(s)
      Niwa Y, Kondo M, Ueno S, Nakamura M, Terasaki H, Miyake Y
    • Journal Title

      Investigative Ophthalmology & Visual Science 46(in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Luminance dependence of neural components that underlies the primate photopic Electroretinogram.2004

    • Author(s)
      Ueno S
    • Journal Title

      Investigative Ophthalmology & Visual Science 45

      Pages: 1033-1040

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Peripheral cone dystrophy : A variant of cone dystrophy with predominant dysfunction in the peripheral cone system.2004

    • Author(s)
      Kondo M
    • Journal Title

      Ophthalmology 111

      Pages: 732-739

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Luminance dependence of neural components that underlines the primate photopic Electroretinogram.2004

    • Author(s)
      Ueno, S.
    • Journal Title

      Investigative Opthalmology & Visual Science. 45

      Pages: 1033-1040

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Peripheral cone dystrophy: A variant of cone dystrophy with predominant dysfunction in the peripheral cone system.2004

    • Author(s)
      Kondo, M.
    • Journal Title

      Ophthalmology 111

      Pages: 732-739

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Luminance dependence of neural components that underlies the primate photopic Electroretinogram.2004

    • Author(s)
      Ueno S, Kondo M, Niwa Y, Terasaki H, Miyake Y.
    • Journal Title

      Investigative Ophthalmology & Visual Science 45

      Pages: 1033-1040

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Peripheral cone dystrophy : A variant of cone dystrophy with predominant dysfunction in the peripheral cone system.2004

    • Author(s)
      Kondo M, Miyake Y, Kondo N, Ueno S, Takakuwa H, Terasaki H.
    • Journal Title

      Ophthalmology 111

      Pages: 732-739

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Effects of flanking gene and genetic background on the phenotypes of mice deficient in basigin/CD147. an immunoglobulin superfamily member.2004

    • Author(s)
      Chen S, Kadomatsu K, Kondo M, Tovama Y, Toshimori K, et al.
    • Journal Title

      Biochemical and Biophysical Research Communications 324

      Pages: 147-153

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Homozygosity-based search for mutations in patients with recessive retinitis pigmentosa using microsatellite markers.2004

    • Author(s)
      Kondo H, Quin M, Mizota A, Kondo M.K. et al.
    • Journal Title

      Investigative Ophthalmology & Visual Science 46

      Pages: 4433-4439

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Changes in focal macular ERGs after macular translocation surgery with 360゜ retinotomy.2004

    • Author(s)
      Terasaki H, Ishikawa K, Niwa Y, Piao CH, Niwa T, Kondo_M, et al.
    • Journal Title

      Investigative Ophthalmology & Visual Science 45

      Pages: 567-573

    • Related Report
      2004 Annual Research Report
  • [Book] どうとる? どう読む? ERG2004

    • Author(s)
      山本修一, 新井三樹, 菅原岳史, 近藤峰生(編集)
    • Total Pages
      179
    • Publisher
      メジカルビュー社
    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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