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"Good basement membrane" will inhibit diabetic retinopathy

Research Project

Project/Area Number 16591755
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionOkayama University

Principal Investigator

KUSACHI Shozo  Okayama University, Medical School, professor, 医学部, 教授 (30214943)

Co-Investigator(Kenkyū-buntansha) NINOMIYA Yoshifumi  Okayama University, Graduate School of Medicine, Dentistry Phamaceutical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (70126241)
HIROHATA Satoshi  Okayama University, Graduate School of Medicine, Dentistry Phamaceutical Sciences, Assistant, 大学院・医歯薬学総合研究科, 助手 (90332791)
SHIRAGA Fumio  Kagawa University, Medical School, Professor, 医学部, 教授 (50187530)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥2,800,000 (Direct Cost: ¥2,800,000)
Keywordsextracellular matrix / laminin / diabetic retinopathy / basement membrane
Research Abstract

Diabetic retinopathy is characterized as increased angiogenesis as well as leaky vessels resulted in abnormal bleeding. The aim of our study was to define the role of basement membrane for diabetic retinopathy. We hypothesized that a breakdown or an abnormal organization of basement membrane was the initial step of the pathological change in the diabetic retinopathy that leads to the vulnerability of the vessels.
We focused on the two components of basement membrane, laminin and type IV collagen.
1. Leaky vessels in diabetic retinopathy
Diabetic rat model was made by administration of streptozotocin (STZ) (180mg/kg, i.p.). The blood glucose level was measured. All the rats demonstrated continued high blood glucose level (>250mg/dl). We examined the retina of the diabetic rat by using Evans blue staining. Despite the diabetic state, our rats did not show particular evidence of leaky vessels.
2. Distribution of basement membrane components in diabetic retinopathy
We examined the distribution of the major components of basement membrane, laminin and type IV collagen. We used specific antibody against from α1 to α6 chains of type IV collagen and α4 and α5 chain of laminin for the immunofluorescent staining analysis.
In the vascular basement membrane of the retina, α1 and α2 chains of type IV collagen was observed, while internal limiting membrane (ILM) showed positive signals for α5 and α6 chains of collagen IV as well as α1 and α2 chains of collagen IV.
3. The change of basement membrane components and the diabetic retinopathy
We concluded that the establishment of proper model for diabetic retinopathy is crucial. The transgenic animal of basement membrane may be of interest.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (15 results)

All 2006 2005 2004

All Journal Article (15 results)

  • [Journal Article] Nicorandil reduces the incidence of minor cardiac marker elevation after coronary stenting2006

    • Author(s)
      Murakami M
    • Journal Title

      Int J Cardiol 107(1)

      Pages: 48-53

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Time-dependent changes in plasma osteopontin levels in patients with anterior-wall acute myocardial infarction after successful rep erfusion : Correlation with left-ventricular volume and function2005

    • Author(s)
      Suezawa C
    • Journal Title

      J Lab Clin Med 145(1)

      Pages: 33-40

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Significant correlation of recruitable coronary collateral blood flow determined by coronary wedge pressure with ST-segment elevation during coronary occlusion2005

    • Author(s)
      Kamikawa S
    • Journal Title

      Coron Artery Dis 16(4)

      Pages: 231-236

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Throinbospondin-1 is induced in rat myocardial infarction and its induction is accelerated by ischemia/reperfusion2005

    • Author(s)
      Sezaki S
    • Journal Title

      Exp Biol Med 230(9)

      Pages: 621-630

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Coronary pressure measurement to determine treatment strategy for equivocal left main coronary artery lesions2005

    • Author(s)
      Suemaru S
    • Journal Title

      Heart Vessels 20(6)

      Pages: 271-277

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Versican is induced in infiltrating monocytes in myocardial infarction2005

    • Author(s)
      Toeda K
    • Journal Title

      Mol Cell Biochem 280(1-2)

      Pages: 41-56

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Time-dependent changes in plasma osteopontin levels in patients with anterior-wall acute myocardial infarction after successful reperfusion : Correlation with left-ventricular volume and function2005

    • Author(s)
      Suezawa C
    • Journal Title

      J Lab Clin Med 145(1)

      Pages: 33-40

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Thrombospondin-1 is induced in rat myocardial infarction and its induction is. accelerated by ischemia/reperfusion2005

    • Author(s)
      Sezaki S
    • Journal Title

      Exp Biol Med 230 (9)

      Pages: 621-630

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Nicorandil reduces the incidence of minor cardiac marker elevation after coronary stenting2005

    • Author(s)
      Murakami M
    • Journal Title

      Int J Cardiol 107 (1)

      Pages: 48-53

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Time-dependent changes in plasma osteopontin levels in patients with an terior-wall acute myocardial infarction after successful reperfusion : Correl ation with left-ventricular volume and function2005

    • Author(s)
      Suezawa C
    • Journal Title

      J Lab Clin Med 145(1)

      Pages: 33-40

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Thrombospondin-1 is induced in rat myocardial infarction and its induction is accelerated by ischemia/reperfusion2005

    • Author(s)
      Sezaki S
    • Journal Title

      Exp Biol Med 230(9)

      Pages: 621-630

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Dynamic Induction of ADAMTS1 Gene in the Early Phase of Acute Myocardial Infarction2004

    • Author(s)
      Nakamura K
    • Journal Title

      J Biochem (Tokyo) 136(4)

      Pages: 439-446

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Circulating white blood cell count correlates with left ventricular indices independently of the extent of risk area for myocardial infarction after successful reperfusion2004

    • Author(s)
      Uesugi T
    • Journal Title

      Acta Cardiol 59(5)

      Pages: 533-539

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Lp3/Hapln3, a novel link protein that co-localizes with versican and is coordinately up-regulated by platelet derived growth factor in arterial smooth muscle cells2004

    • Author(s)
      Ogawa H
    • Journal Title

      Matrix Biol 23(5)

      Pages: 287-298

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Coronary flow reserve after coronary intervention in similar in patients with preserved viability in previous myocardial infarction and in those with angina pectoris2004

    • Author(s)
      Takaishi A
    • Journal Title

      J Int Med Res 32(3)

      Pages: 245-257

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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