| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2004: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Research Abstract |
Proteomics analysis was performed for the screening of proteins, related with retina survival, differentiation and regeneration Many proteins, including transcriptional factors, structural proteins, apoptosis factor, differentiation factors and novel proteins, were identified. Among of them, we focus on the Wnt14, one of the protein family for the differentiation, and the cell signaling of Wnt14 was analyzed. Immunohistochemistry shows that Wnt14 was endogeneously located in retinal ganglion cells of chick embryo retina. And, overexpressed Wnt14 inhibit cell death of R28 cells, rat retinal cell line, induced by glutamate neuronal toxity. Moreover, Wnt14 have the neuronal protective effect through the inhibition of activated caspase-3. apoptosis inducer.
On the other hand, we also newly porteomics, using glaucoma model mouse retina, to find out the proteins associated with retinal survival factor in glaucoma. In result, the protein targets for glaucomatous retina degeneration were identified. PDGFR, one of the identified protein, was located in retinal ganglion cells on mouse retina, and PDGF-AA, specific ligand for PDGFR, have the neuro protective effect on the cell death of RGC-5, rat retina ganglion cell line, induced by glutamate. Finally, we succeeded in finding out newly neurotrophic proteins for retina.
In addition, we check the genomic DNA of glaucoma patients, and we found polymorphism of TNF-alpha, Angiotensin-II receptor, Endothelin A receptor were associated with glaucoma. Otherwise, ApoE polymorphism, seen in Alzheimer disease, have no relation with glaucoma
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