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The development for new inhibitory methods for corneal graft rejection by using NKT cells

Research Project

Project/Area Number 16591757
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

SONODA Koh-Hei  Kyushu University, Graduate School of Medical Science, Assistant Professor, 大学院・医学研究院, 助手 (10294943)

Co-Investigator(Kenkyū-buntansha) YOSHIDA Hiroki  Saga University, Faculty of Medicine, Professor, 医学部・分子生命科学講座, 教授 (40260715)
EGASHIRA Kensuke  Kyushu University, Graduate School of Medical Science, Assosiate Professor, 大学院・医学研究院, 助教授 (60260379)
HATA Yasuaki  Kyushu University, Graduate School of Medical Science, Assistant Professor, 大学院・医学研究院, 講師 (90346776)
YOSHIDA Shigeo  Kyushu University, Graduate School of Medical Science, Assistant Professor, 大学院・医学研究院, 助手 (50363370)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2004: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordscorneal transplantation / NKT cell / cytokine / tolerance / DNA chip / gene transfer / immune therapy
Research Abstract

Analysis in murine corneal allo graft (in vivo)
NKT cells were enriched from spleen and peripheral blood of grafted mice seven days later. NKT cells were enriched by magnetic beads. We analyzed cytokine and chemokine produced from these NKT cells. NKT cells were cultured with IL-2 and IL-15 and stimulated by aGalCer. We found NKT cells produced IL-10.
The expansion of human NKT cells (in vitro)
NKT cells were enriched from either long term accepted patients or healthy volunteers. We enriched NKT cells with more than 90% purity by magnetic beads. As we did in mice, NKT cells were cultured and expanded by aGalCer with IL-2 and IL-15.
The screening of cytokine and chemokines produced by NKT cells
We screened specific cytokines and chemokines in graft-accepted patients. We found NKT cells from accepted patients predominantly produced IL-10. In contrast, they reduced IFNg, TNFa and RANTES production.
Gene transfer of IL-10 into cultured NKT cells
We succeeded to transfer IL-10 gene into cultured human NKT cells, and confirmed IL-10 production in vitro.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (1 results)

All 2005

All Journal Article (1 results)

  • [Journal Article] The cellular events in normal and inflammatory conditions at cornea2005

    • Author(s)
      Sonoda K-H, Nakao S et al.
    • Journal Title

      cornea 24.8

    • Related Report
      2005 Annual Research Report

URL: 

Published: 2004-04-01   Modified: 2016-04-21  

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