Identification of the gene expression profile specific for Behcet's disease with uveitis and the functional analysis
| Project/Area Number |
16591769
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Tokyo Medical University |
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Principal Investigator |
TAKEUCHI Masaru Tokyo Medical University, Ophthalmology, assistant professor, MD, PhD, 医学部, 講師 (40260939)
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| Co-Investigator(Kenkyū-buntansha) |
KEZUKA Takeshi Tokyo Medical University, Ophthalmology, assistant professor, MD, PhD, 医学部, 講師 (00287137)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2004: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Behcet's disease / uveitis / autoimmune disease / microarray / chemokine / experimental autoimmune uveitis |
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| Research Abstract |
Purpose : Development of organ-specific autoimmune diseases, including uveitis, is thought to be regulated by signals transmitted between local tissue-specific cells and circulating immune cells. In this study, we attempted to identify chemoattractive signals specific for the development of uveitis in behcet's disease (BD) by by comparing total gene expression in experimental autoimmune uveoretinitis (EAU).
Methods : C57BL/6 mice were immunized with 200 mg of BSA, peptide 35-55 of myelin oligodendrocyte protein (MOG-p), or hIRBP-p emulsified in complete Freund's adjuvant. On day 14, retinas and splenic T cells were collected, and total RNAs were extracted. The expression profiles of approximately 20,000 potentially relevant genes were analyzed using pooled RNA samples within individual groups and the Codelink Bioarray system. The data were evaluated by the gene expression ratio of each experimental group versus non-immunized control group. In addition, CXCL7 mRNA expression was analyzed
… More
in periphral monocytes in 15 BD patients by real time PCR.
Results : In the retina of mice immunized with hIRBP-p that induce EAU, genes of CCL5, CXCL9, and CXCL10 related to Th1 cell recruitment and those of CCL7, CCL8 and CCL17 associated with Th2 cell recruitment were preferentially expressed compared with other chemokine genes. Although these chemokine genes were also expressed in the retina of mice immunized with MOG-p that induce optic neuritis in addition to experimental autoimmune encephalomyelitis, the overall level of expression was lower than that in the retina with induced EAU. In T cells, EAU-specific Th1 or Th2 chemokine gene expression was not observed. Interestingly, gene of CCL2, CCL24 or CXCL7 responsible for macrophage and neutrophil recruitment was specifically detected in the retina and T cells of mice with induced EAU and not in the other immunized groups. CXCL7 mRNA expression was increased in BD with active uveitis compared with BD with uveitis on remission or healthy contro Less
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Report
(3 results)
Research Products
(18 results)
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[Journal Article] Frequent immune response to a melanocyte-specific protein KU-MEL-1 in patients with Vogt-Koyanagi-Harada disease.2006
Author(s)
Otani S, Sakurai T, Yamaoto K, Fujita T, Matsuzaki Y, Goto Y, Ando Y, Suzuki S, Usui M, Takeuchi M, Kawakami Y
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Journal Title
British Journal of Ophthalmology (In press)
Description
「研究成果報告書概要(和文)」より
Related Report
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[Journal Article] CCR5-deficient mice develop experimental autoimmune uveoretinitis in the context effector response.2005
Author(s)
Takeuchi A, Usui Y, Takeuchi M, Hattori T, Kezuka T, Suzuki J, Okunuki Y, Iwasaki T, Haino M, Matsushima K, Usui M
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Journal Title
Investigative Ophthalmology and Visual Science 46(10)
Pages: 3753-60
Description
「研究成果報告書概要(和文)」より
Related Report
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[Journal Article] CCR5-deficient mice develop experimental autoimmune uveoretinitis in the context of a deviant effector response.2005
Author(s)
Takeuchi A, Usui Y, Takeuchi M, Hattori T, Kezuka T, Suzuki J, Okunuki Y, Iwasaki T, Haino M, Matsushima K, Usui M.
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Journal Title
Investigative Ophthalmology and Visual Science 46(10)
Pages: 3753-3760
Description
「研究成果報告書概要(欧文)」より
Related Report
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