| Project/Area Number |
16591775
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Kinki University |
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Principal Investigator |
HIGAKI Shiro Kinki University, School of Medicine, Assistant Professor, 医学部, 講師 (90238262)
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| Co-Investigator(Kenkyū-buntansha) |
FUKUDA Masahiko Kinki University, School of Medicine, Assistant Professor, 医学部, 講師 (40218938)
SHIMOMURA Yoshikazu Kinki University, School of Medicine, Professor, 医学部, 教授 (20162737)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2005: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2004: ¥500,000 (Direct Cost: ¥500,000)
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| Keywords | HSV-1 / Herpetic keratitis / Reactivation / Latency |
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| Research Abstract |
Herpes simplex virus type 1 (HSV-1) establishes a latent state in sensory neurons and may reactivate throughout the life of the host
We investigated the method to stop reactivation of HSV-1.
The mouse corneas were scarified and inoculated with 3 □l of a viral suspension containing 2.5 X 10^4 plaque-forming units of HSV-1 strain McKrae. To verify corneal infection, slit lamp examinations and ocular swabs were performed on days 3 and 4 after inoculation. The mice were treated by Valacyclovir (VACV) in acute phase. In VACV treated group compared to non-treated one, the rate of HSV reactivation was significantly lower.
If substances that regulate these genes can be shown to block viral reactivation, such agents could form a new class of antiviral medication.
Herpetic keratitis was classified into dendritic ulcer, geographica, endotheliatis, etc. the therapy for these lesion is well established. Now, how to stop reactivation is very important.
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